Difference between revisions of "Team:Northeastern Boston/BasicParts"
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<h1 id="project_header" style="border-width:0">BASIC & COMPOSITE PARTS</h1> | <h1 id="project_header" style="border-width:0">BASIC & COMPOSITE PARTS</h1> | ||
| − | <h2>Hygromycin B Resistance Cassette</h2> | + | <h2>Hygromycin B Resistance Cassette <a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K1857001" target="_blank" style="color:white">BBa_K1857001</a></h2> |
<img src="https://static.igem.org/mediawiki/2015/0/0c/HygResCassettetake14-01.jpg" style="width:100%"> | <img src="https://static.igem.org/mediawiki/2015/0/0c/HygResCassettetake14-01.jpg" style="width:100%"> | ||
Revision as of 00:49, 17 September 2015
BASIC & COMPOSITE PARTS
Hygromycin B Resistance Cassette BBa_K1857001
Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygamycin B resistance cassette. This composite part was cloned from pOpt_mVenus_Hyg and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any Chlamydomonas reinhardtii transformed with this part.
This part contains intron 2 of RBCS2 after a fusion HSP70A-RBCS2 promoter. [1] Aph7'', a phosphotransferase, confers resistance to Hygromycin B at agar antibiotic concentrations up to 200g/mL. [2]
mVenus Fluorescent Protein
This part contains mVenus, a nuclear codon-optimized YFP variant (excitation/emission: 515/528nm) [3]. It has Intron 1 and 2 of RBCS2 for increased expression. This is the first independent fluorescent protein codon-optimized for Chlamydomonas reinhardtii submitted to the iGEM registry.