Difference between revisions of "Team:Nankai/Basic Part"

Line 107: Line 107:
 
<div class="col-md-8 blog-posts">
 
<div class="col-md-8 blog-posts">
 
<h4>Basic Parts</h4>
 
<h4>Basic Parts</h4>
−
<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628001">BBa_K1628001</a> (P<sub>bca</sub>)<br/>
+
<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px; font-weight: bold;">Promoters</p>
−
Promoter P<sub>bca</sub> is the original promoter of <em>pgsBCA</em> operon. According to our promoter strength assay (showed in Figure 1), the strength of P<sub>bca</sub> is very weak.</p>
+
<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;"> According to our promoter strength assay (showed in Figure 1), <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628001">BBa_K1628001</a> (P<sub>bca</sub>), <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628003">BBa_K1628003</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (BJ27UP<sub></sub>), <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628004">BBa_K1628004</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (C2up<sub></sub>), <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628005">BBa_K1628005</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (A2up<sub></sub>), <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628006">BBa_K1628006</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (P43), and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628007">BBa_K1628007</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (P<sub>amyA</sub>) show different properties. Promoter P<sub>bca</sub> is the original promoter of <em>pgsBCA</em> operon.Promoter BJ27UP C2up and A2up are an artificially synthesized promoters. Promoter P43 is a strong promoter in <em>Bacillus subtilis</em> 168. Promoter P<sub>amyA</sub> is a strong promoter in <em>Bacillus amyloliquefaciens</em> LL3.</p>
−
<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002">BBa_K1628002</a> (P<sub>xyl</sub>)<br/>
+
<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;">Judging from the promoter strength assay, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628004">BBa_K1628004</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (C2up<sub></sub>)<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> is the strongest promoters we used in our project and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628007">BBa_K1628007</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (P<sub>amyA</sub>) is the second strongest promoter in our project. The strength of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628001">BBa_K1628001</a> (P<sub>bca</sub>) is very weak. While the strength of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628003">BBa_K1628003</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (BJ27UP<sub></sub>) is stronger than P<sub>bca</sub>, it is still too weak compared with other promoters. What's more, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628005">BBa_K1628005</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (A2up<sub></sub>) is a very strong promoter and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628006">BBa_K1628006</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (P43) is a weak promoter.</p>
−
  Promoter P<sub>xyl</sub> is a promoter of xylose operon regulate by repressor XylR. Promoter P<sub>xyl</sub> together with promoter P<sub>lacI</sub>, repressor LacI (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628201">BBa_K1628201</a>), promoter P<sub>grac</sub> (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628202">BBa_K1628202</a>) and repressor XylR (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628203">BBa_K1628203</a>) formed a metabolic toggle switch. We used this device to regulate the expression of <em>odhAB</em> genes in <em>B. amyloliquefaciens</em> NK-1 (showed in Figure 2). Without IPTG, the promoter P<sub>grac</sub> is inhibited by suppressor LacI and the supreessor XylR will not synthesized, thus the promoter P<sub>xyl</sub> is active and <em>odhAB</em> genes are expressed. When IPTG is added, the<em> xylR</em> gene is expressed and the suppressor XylR is synthesized thereafter inhibited the expression of <em>odhAB</em> genes.</p>
+
<p><img src="https://static.igem.org/mediawiki/2015/c/c6/Nankai_parts_figure1_blue.jpeg" width="561"></p>
−
<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628003">BBa_K1628003</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (BJ27UP <sub></sub>)<br/>
+
<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;"> Figure 1. Promoter strength assay in <em>Bacillus amyloliquefaciens</em> NK-1.</p>
−
  Promoter BJ27UP is an artificially synthesized promoter. According to our promoter strength assay in <em>B. amyloliquefaciens</em> NK-1 (showed in Figure 1), the strength of BJ27UP is stronger than Pbcabut is still too weak compared with other promoters. </p>
+
<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px; font-weight: bold;">Coding genes</p>
−
<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628004">BBa_K1628004</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (C2up<sub></sub>)<br/>
+
<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628101">BBa_K1628101</a> (<em>pgsB</em>) and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628102">BBa_K1628102</a> (<em>pgsAC</em>) are genes in <em>pgsBCA</em> operon. <em>pgsB</em> is a gene responsible for γ-PGA synthesis.Protein PgsBCA is a membrane protein and subunit PgsB’s main function is gathering substrate glutamic acid for γ-PGA synthesis (showed in Figure 3). Subunit <em>PgsC</em> is responsible for glutamic acid’s polymerization and subunit <em>PgsA</em> is responsible for the secretion of γ-PGA.</p>
−
  Promoter C2up is an artificially synthesized promoter. According to our promoter strength assay in <em>B. amyloliquefaciens</em> NK-1 (showed in Figure 1), C2up is the strongest promoters we used in our project.</p>
+
<p><img src="https://static.igem.org/mediawiki/2015/2/25/Parts_figure3.jpeg" width="714" height="435"></p>
−
<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628005">BBa_K1628005</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (A2up<sub></sub>)<br/>
+
−
  Promoter A2cup is an artificially synthesized promoter. According to our promoter strength assay in <em>B. amyloliquefaciens</em> NK-1 (showed in Figure 1), A2up is a very strong promoter in <em>B. amyloliquefaciens</em> NK-1.</p>
+
−
<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628006">BBa_K1628006</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (P43)<br/>
+
−
  Promoter P43 is a strong promoter in <em>Bacillus subtilis</em> 168. According to our promoter strength assay in (showed in Figure 1), P43 is a weak promoter in <em>B. amyloliquefaciens</em> NK-1.</p>
+
−
<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628007">BBa_K1628007</a><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002"></a> (P<sub>amyA</sub>)<br/>
+
−
  Promoter P<sub>amyA</sub> is a strong promoter in <em>Bacillus amyloliquefaciens</em> LL3. According to our promoter strength assay in (showed in Figure 1), P<sub>amyA</sub>is the second strongest promoter in our project.</p>
+
−
<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628101">BBa_K1628101</a> (<em>pgsB</em>)<br/>
+
−
  <em>pgsB</em> is a gene responsible for γ-PGA synthesis in <em>pgsBCA</em> operon. Protein PgsBCA is a membrane protein and subunit PgsB’s main function is gathering substrate glutamic acid for γ-PGA synthesis (showed in Figure 3). </p>
+
−
<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628102">BBa_K1628102</a> (<em>pgsAC</em>)<br/>
+
−
  <em>pgsC</em> and <em>pgsA</em> are two genes in <em>pgsBCA</em> operon. Protein PgsBCA is a membrane protein responsible for the synthesis of γ-PGA. Subunit <em>PgsC</em> is responsible for glutamic acid’s polymerization and subunit <em>PgsA</em> is responsible for the secretion of γ-PGA (showed in Figure 3)</p>
+
−
<p>&nbsp;</p>
+
−
<p>&nbsp;</p>
+
−
<img src="https://static.igem.org/mediawiki/2015/b/be/Partsfigure_new4.jpeg" width="561">
+
−
                                                <p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;"> Figure 1. Promoter strength assay in <em>Bacillus amyloliquefaciens</em> NK-1.             </p>
+
−
              <p><img src="https://static.igem.org/mediawiki/2015/2/25/Parts_figure3.jpeg" width="714" height="435"></p>
+
 
               <p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;">Figure 3. The synthetic pathway of γ-PGA</p>
 
               <p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;">Figure 3. The synthetic pathway of γ-PGA</p>
 
               <p>&nbsp;</p>
 
               <p>&nbsp;</p>

Revision as of 02:14, 17 September 2015

Medigo Blue, free responsive template

Your place: Home > Parts > Basic Part

Basic Parts

Basic Parts

Promoters

According to our promoter strength assay (showed in Figure 1), BBa_K1628001 (Pbca), BBa_K1628003 (BJ27UP), BBa_K1628004 (C2up), BBa_K1628005 (A2up), BBa_K1628006 (P43), and BBa_K1628007 (PamyA) show different properties. Promoter Pbca is the original promoter of pgsBCA operon.Promoter BJ27UP C2up and A2up are an artificially synthesized promoters. Promoter P43 is a strong promoter in Bacillus subtilis 168. Promoter PamyA is a strong promoter in Bacillus amyloliquefaciens LL3.

Judging from the promoter strength assay, BBa_K1628004 (C2up) is the strongest promoters we used in our project and BBa_K1628007 (PamyA) is the second strongest promoter in our project. The strength of BBa_K1628001 (Pbca) is very weak. While the strength of BBa_K1628003 (BJ27UP) is stronger than Pbca, it is still too weak compared with other promoters. What's more, BBa_K1628005 (A2up) is a very strong promoter and BBa_K1628006 (P43) is a weak promoter.

Figure 1. Promoter strength assay in Bacillus amyloliquefaciens NK-1.

Coding genes

BBa_K1628101 (pgsB) and BBa_K1628102 (pgsAC) are genes in pgsBCA operon. pgsB is a gene responsible for γ-PGA synthesis.Protein PgsBCA is a membrane protein and subunit PgsB’s main function is gathering substrate glutamic acid for γ-PGA synthesis (showed in Figure 3). Subunit PgsC is responsible for glutamic acid’s polymerization and subunit PgsA is responsible for the secretion of γ-PGA.

Figure 3. The synthetic pathway of γ-PGA

 

 

 

Team Parts
Basic Parts
Composite Parts
Part Collection

     

     

     

     
    Medigo Blue, free responsive template

    Copyright © Nankai University

    Nankai IGEM Team 2015