Difference between revisions of "Team:Shiyan SY China/Parts"

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                             <h3><a target="_blank" style="cursor: pointer;" onclick="location.href ='https://2015.igem.org/Team:Shiyan_SY_China/Project.html';"title="">PROJECT</a></h3>
 
                             <h3><a target="_blank" style="cursor: pointer;" onclick="location.href ='https://2015.igem.org/Team:Shiyan_SY_China/Project.html';"title="">PROJECT</a></h3>
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                             <ul style="margin:0px auto;" class="sub">
 
                        <li ><a style ="background:#27AE60" href="https://2015.igem.org/Team:Shiyan_SY_China/Project.html#t1">Description</a> </li>
 
                        <li ><a style ="background:#27AE60" href="https://2015.igem.org/Team:Shiyan_SY_China/Project.html#t1">Description</a> </li>
 
                        <li ><a style ="background:#27AE60" href="https://2015.igem.org/Team:Shiyan_SY_China/Project.html#t2">Background</a> </li>
 
                        <li ><a style ="background:#27AE60" href="https://2015.igem.org/Team:Shiyan_SY_China/Project.html#t2">Background</a> </li>
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                             <li ><a style ="background:#27AE60" href="https://2015.igem.org/Team:Shiyan_SY_China/Project.html#t6">Parts</a> </li>
 
                             <li ><a style ="background:#27AE60" href="https://2015.igem.org/Team:Shiyan_SY_China/Project.html#t6">Parts</a> </li>
 
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                             <h3 style = "line-height: 2;"><a target="_blank" style="cursor: pointer;back" onclick="location.href ='https://2015.igem.org/Team:Shiyan_SY_China/Practice.html';"  title="">HUMAN PRACTICES</a></h3>
 
                             <h3 style = "line-height: 2;"><a target="_blank" style="cursor: pointer;back" onclick="location.href ='https://2015.igem.org/Team:Shiyan_SY_China/Practice.html';"  title="">HUMAN PRACTICES</a></h3>
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                        <li ><a style ="background:#F39C12" href="https://2015.igem.org/Team:Shiyan_SY_China/Practice.html#t1">T-shirt</a> </li>
 
                        <li ><a style ="background:#F39C12" href="https://2015.igem.org/Team:Shiyan_SY_China/Practice.html#t1">T-shirt</a> </li>
 
                             <li ><a style ="background:#F39C12" href="https://2015.igem.org/Team:Shiyan_SY_China/Practice.html#t2">Biology Speech</a> </li>
 
                             <li ><a style ="background:#F39C12" href="https://2015.igem.org/Team:Shiyan_SY_China/Practice.html#t2">Biology Speech</a> </li>
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                             <h3><a target="_blank" style="cursor: pointer;" onclick="location.href ='https://2015.igem.org/Team:Shiyan_SY_China/Safety.html';" title="">SAFETY</a></h3>
 
                             <h3><a target="_blank" style="cursor: pointer;" onclick="location.href ='https://2015.igem.org/Team:Shiyan_SY_China/Safety.html';" title="">SAFETY</a></h3>
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                        <li ><a style ="background:#BE382A" href="https://2015.igem.org/Team:Shiyan_SY_China/Safety.html#t1">Safety Design</a> </li>
 
                        <li ><a style ="background:#BE382A" href="https://2015.igem.org/Team:Shiyan_SY_China/Safety.html#t1">Safety Design</a> </li>
 
                        <li ><a style ="background:#BE382A" href="https://2015.igem.org/Team:Shiyan_SY_China/Safety.html#t2">Lab security</a> </li>
 
                        <li ><a style ="background:#BE382A" href="https://2015.igem.org/Team:Shiyan_SY_China/Safety.html#t2">Lab security</a> </li>
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                             <h3><a target="_blank" style="cursor: pointer;" onclick="location.href ='https://2015.igem.org/Team:Shiyan_SY_China/Team.html';"  title="">TEAM</a></h3>
 
                             <h3><a target="_blank" style="cursor: pointer;" onclick="location.href ='https://2015.igem.org/Team:Shiyan_SY_China/Team.html';"  title="">TEAM</a></h3>
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                        <li ><a style ="background:#8E44AD" href="https://2015.igem.org/Team:Shiyan_SY_China/Team.html#t1">Members</a> </li>
 
                        <li ><a style ="background:#8E44AD" href="https://2015.igem.org/Team:Shiyan_SY_China/Team.html#t1">Members</a> </li>
 
                             <li ><a style ="background:#8E44AD" href="https://2015.igem.org/Team:Shiyan_SY_China/Team.html#t2">Instructors</a> </li>
 
                             <li ><a style ="background:#8E44AD" href="https://2015.igem.org/Team:Shiyan_SY_China/Team.html#t2">Instructors</a> </li>
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                             <h3><a target="_blank" style="cursor: pointer;" onclick="location.href ='https://2015.igem.org/Team:Shiyan_SY_China/Notebook.html';"  title="">NOTEBOOK</a></h3>
 
                             <h3><a target="_blank" style="cursor: pointer;" onclick="location.href ='https://2015.igem.org/Team:Shiyan_SY_China/Notebook.html';"  title="">NOTEBOOK</a></h3>
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                        <li ><a style ="background:#0498F9" href="https://2015.igem.org/Team:Shiyan_SY_China/Notebook.html#t1">Notebook</a> </li>
  
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This part includes two individual DNA domains. 1. A strong promoter followed with a RNA thermometer sequence, which regulate the transcription of opdA gene and expression of opdA protein out of the host cell via an ompA peptide. This DNA fragment domain could be obtained by double-digested with EcoR I + Spe I. 2. A UV induced promoter (RecA SOS) followed with a suicide gene ccdB, which could lead to the death of host cell if exposed in UV. This DNA fragment domain could be obtained by double-digested with Xho I + Spe I. And the whole part DNA fragment could be obtained by double-digested with Xho I + EcoR I.  
+
This part includes two individual DNA domains. Constitutive promoter tunes the expression of downstream opdA gene with further help from RNA thermometer. RNA thermometer provides a temperature sensitive post-transcriptional regulation on opdA gene, which initiate the opdA translation around 32°C. OmpA signal peptide guides the secretion of opdA protein to the outside of host strain. Then opdA enzyme specifically degrades organophosphorus pesticide appeared, through hydrolysis. Upon UV light, RecA（SOS）promoter drives the transcription of downstream ccdB suicide gene, whose protein expression interferes DNA sysnthesis and lead to cell dealth. In this way, we can wipe out our genetic engineered bacteria by giving UV lights under manual control to avoid secondary pollution. Without UV light, RecA promoter won’t be activated, so the normal growth and activity of genetic engineering bacteria will be preserved well to release functional opdA protein under temperature control. </p>
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<div>&nbsp;</div>
 
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<h style="font-size:15px;"> More information please see our parts form:  </h>
 
<h style="font-size:15px;"> More information please see our parts form:  </h>
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<td style="text-align:left;border-top:1px solid #414141;"> 1776</td>
 
<td style="text-align:left;border-top:1px solid #414141;"> 1776</td>
 
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<p>© 2015 Shiyan_SY_China iGEM Team. All rights reserved.  
 
<p>© 2015 Shiyan_SY_China iGEM Team. All rights reserved.  

Revision as of 02:44, 17 September 2015

IGEM-PROJECT

You have to believe in yourself. That’s the secret of success.

—— Charles Chaplin

Parts
Our submission parts:
 
BBa_K1667005

This part includes two individual DNA domains. Constitutive promoter tunes the expression of downstream opdA gene with further help from RNA thermometer. RNA thermometer provides a temperature sensitive post-transcriptional regulation on opdA gene, which initiate the opdA translation around 32°C. OmpA signal peptide guides the secretion of opdA protein to the outside of host strain. Then opdA enzyme specifically degrades organophosphorus pesticide appeared, through hydrolysis. Upon UV light, RecA（SOS）promoter drives the transcription of downstream ccdB suicide gene, whose protein expression interferes DNA sysnthesis and lead to cell dealth. In this way, we can wipe out our genetic engineered bacteria by giving UV lights under manual control to avoid secondary pollution. Without UV light, RecA promoter won’t be activated, so the normal growth and activity of genetic engineering bacteria will be preserved well to release functional opdA protein under temperature control.

 
More information please see our parts form:
 
Name Type Description Length
BBa_K1667005 DNA OpdA encoding gene with ompA signal peptide 1776
 

© 2015 Shiyan_SY_China iGEM Team. All rights reserved.