Difference between revisions of "Team:Northeastern Boston/BasicParts"
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| − | <div id='bar_title' class='title'><a href="https://2015.igem.org/Team:Northeastern_Boston | + | <div id='bar_title' class='title'><a href="https://2015.igem.org/Team:Northeastern_Boston"> |
Northeastern</a> | Northeastern</a> | ||
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| − | <a href="https://2015.igem.org/Team:Northeastern_Boston">Home</a> | + | <a id='a-home' href="https://2015.igem.org/Team:Northeastern_Boston">Home</a> |
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| − | <a href="https://2015.igem.org/Team:Northeastern_Boston/team">Team</a> | + | <a id='a-team' href="https://2015.igem.org/Team:Northeastern_Boston/team">Team</a> |
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| − | <a>Parts<div class= | + | <a> |
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| − | <a href="https://2015.igem.org/Team:Northeastern_Boston/safety">Safety</a> | + | <a id="a-safety" href="https://2015.igem.org/Team:Northeastern_Boston/safety">Safety</a> |
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| − | <a href="https://2015.igem.org/Team:Northeastern_Boston/measurement">Measurement</a> | + | <a id="a-measurement" href="https://2015.igem.org/Team:Northeastern_Boston/measurement">Measurement</a> |
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Revision as of 04:02, 17 September 2015
BASIC & COMPOSITE PARTS
BBa_K1857001 Hygromycin B Resistance Cassette
Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygamycin B resistance cassette. This composite part was cloned from pOpt_mVenus_Hyg and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any Chlamydomonas reinhardtii transformed with this part.
This part contains intron 2 of RBCS2 after a fusion HSP70A-RBCS2 promoter. [1] Aph7'', a phosphotransferase, confers resistance to Hygromycin B at agar antibiotic concentrations up to 200g/mL. [2]
BBa_K1857000 mVenus Fluorescent Protein
This part contains mVenus, a nuclear codon-optimized YFP variant (excitation/emission: 515/528nm) [3]. It has Intron 1 and 2 of RBCS2 for increased expression. This is the first independent fluorescent protein codon-optimized for Chlamydomonas reinhardtii submitted to the iGEM registry.