Difference between revisions of "Team:Wellesley TheTech/Project/Wetlab"
| Line 77: | Line 77: | ||
<p>Optimized to be accessible/easy for walk-up museum visitors of all ages and skill levels: | <p>Optimized to be accessible/easy for walk-up museum visitors of all ages and skill levels: | ||
<br> | <br> | ||
| − | <u>Reagents (pre-made by staff):</u><br> | + | <b><u>Reagents (pre-made by staff):</u></b><br> |
| − | - 6cm LB-agar plates with 25 ug/ml Chloramphenicol<br> | + | - 6cm LB-agar plates with 25 ug/ml Chloramphenicol<br> |
| − | - Plasmid DNA in 100ul CaCl2<br> | + | - Plasmid DNA in 100ul CaCl2<br> |
| − | - 20 ul competent bacteria<br> | + | - 20 ul competent bacteria<br> |
| − | + | <b><u>Transformation protocol (done by visitors):</u></b><br> | |
| + | - Combine 100 DNA with 20 ul of competent bacteria<br> | ||
| + | - 30 sec on ice<br> | ||
| + | - 40 sec heat shock at 42 degrees C<br> | ||
| + | - Plate out bacteria and put in incubator<br> | ||
</p> | </p> | ||
Revision as of 04:40, 17 September 2015
WETLAB COMPONENT
Description of above image
Goal
The goal of the wetlab component of BacPack is to connect the digital experience to a hands-on, lab-based one. In the digital activity visitors explore designing bacteria to have a variety of new, useful functions for helping humans survive in different extreme environments. In the wetlab activity visitors get try their hands at the same real-world process of engineering a bacteria to have a new property of their choosing.
Activity Design Outline
Visitors transform a living bacteria to make it do something new:
-5 DNA options that change the physical appearance of bacteria in different ways
-Each ‘color’ represents a bacteria function that can provide one of the 5 needs on Mars
-Visitors pick the DNA they want to use and transform e.coli with it
-They can see a photo of their grown ‘mars bacteria’ several days later
Development
Plasmids:
Selected 5 bioBricks from the iGEM distribution kit:
-Lux Operon (BBa_K325909) - Bioluminescence pathway
amilGFP (BBa_K592010) - Yellow chromoprotein
aeBlue (BBa_K864401) - Blue chromoprotein
eforRed (BBa_K592012) - Red/pink chromoprotein
asPink (BBa_K1033933) - Pink/purple chromoprotein
Transformation
Optimized to be accessible/easy for walk-up museum visitors of all ages and skill levels:
Reagents (pre-made by staff):
- 6cm LB-agar plates with 25 ug/ml Chloramphenicol
- Plasmid DNA in 100ul CaCl2
- 20 ul competent bacteria
Transformation protocol (done by visitors):
- Combine 100 DNA with 20 ul of competent bacteria
- 30 sec on ice
- 40 sec heat shock at 42 degrees C
- Plate out bacteria and put in incubator