Difference between revisions of "Team:TecCEM/Our parts"

 
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<div class="floatleft"><a href="https://static.igem.org/mediawiki/2015/0/05/Team_TecCEM_banner.jpg" class="image"><img alt="Bannerteccem.jpg" src="https://static.igem.org/mediawiki/2015/0/05/Team_TecCEM_banner.jpg" width="824" height="370" /></a></div> 
 
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<ul>
 
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<a href="https://2015.igem.org/Team:TecCEM"><li>HOME</li></a>
 
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<a href="#"><li>TEAM
 
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            <ul>
 
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<a href="https://2015.igem.org/Team:TecCEM/Team_description"><li>Team description</li></a>
 
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<a href="https://2015.igem.org/Team:TecCEM/Team_profiles"><li>Team profiles</li></a> 
 
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<a href="#"><li>PROJECT
 
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<a href="https://2015.igem.org/Team:TecCEM/Overview"><li>Overview</li></a>
 
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<a href="https://2015.igem.org/Team:TecCEM/Modelling"><li>Modelling</li></a>
 
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<a href="https://2015.igem.org/Team:TecCEM/ Materials_methods"><li>Materials & methods</li></a>
 
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<a href="https://2015.igem.org/Team:TecCEM/ Experimental_design"><li>Experimental design</li></a> 
 
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<a href="https://2015.igem.org/Team:TecCEM/Results_discussion"><li> Results & discussion </li></a> 
 
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<a href="https://2015.igem.org/Team:TecCEM/Conclusions"><li>Conclusions</li></a> 
 
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<a href="#"><li>PARTS
 
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            <ul><a href="https://2015.igem.org/Team:TecCEM/Our_parts"><li>Our parts</li></a>
 
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        <a href="https://2015.igem.org/Team:TecCEM/Parts_interest"><li>Parts of interest</li></a>
 
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<a href="https://2015.igem.org/Team:TecCEM/Collaboration"><li>COLLABORATION</li></a>
 
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<a href="#"><li>NOTEBOOK
 
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            <ul><a href="https://2015.igem.org/Team:TecCEM/2014"><li>2014</li></a>
 
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    <a href="https://2015.igem.org/Team:TecCEM/2015"><li>2015</li></a> 
 
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<a href="https://2015.igem.org/Team:TecCEM/Lab_Safety"><li>LAB SAFETY</li></a>
 
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<a href="https://2015.igem.org/Team:TecCEM/Attributions"><li>ATTRIBUTIONS</li></a>
 
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<a href="https://2015.igem.org/Team:TecCEM/HPractices"><li>HUMAN PRACTICES</li></a>
 
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<a href="https://2015.igem.org/Team:TecCEM/INTERLAB"><li>INTERLAB
 
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            <ul>
 
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<a href="https://2015.igem.org/Team:TecCEM/Study_results"><li>Study & results</li></a>
 
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<a href="https://2015.igem.org/Team:TecCEM/Annex"><li>Annex</li></a> 
 
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<h2>Our parts</h2>
 
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<h3>BBa_K1636000: Aptamer</h3>
 
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This aptameric sequence is capable of recognising and binding specifically to lead II ions. This sequence, which works as a single stranded nucleic acid molecule, is capable of forming a G-quadruplex by interacting with lead ions (Pb2+) while disrupting a partially complementary duplex. For this competition purposes, its design was done in order to standardise it as a biobrick even though it is only functional as single stranded DNA. However, a primer was designed so that it can be retrieved from the biobrick cassette as a ssDNA molecule via an asymmetrical PCR.
 
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<h3>BBa_K1636002: Linker</h3>
 
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This linker was designed by the iGEM TecCEM team in order to retrieve single-stranded oligonucleotides when produced as single-stranded genetic material (e.g. plasmid). The linker should be flanking said oligonucleotides, and given the design of its sequence, it is able to form a hairpin-like secondary structure that allows the usage of a restriction enzyme (MlyI from New England BIolabs) with a blunt cut thereby releasing the oligos of interest.
 
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<h3>BBa_K1636001: Primer for aptamer</h3>
 
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This biobrick was designed in order to perform and asymmetrical PCR. The main aim of this part is the generation of high-copy oligos including the aptamer.  Therefore, it is intimately related to the BBa_K1636000.
 
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Latest revision as of 05:49, 17 September 2015