Difference between revisions of "Team:Aalto-Helsinki/Experiments"
m (listing protocols) |
m (cleaned links) |
||
(15 intermediate revisions by 4 users not shown) | |||
Line 2: | Line 2: | ||
<html> | <html> | ||
</p></div> | </p></div> | ||
+ | |||
+ | |||
+ | <style type="text/css"> | ||
+ | |||
+ | /* Background pic */ | ||
+ | .plasmids { | ||
+ | background-repeat: repeat-y; | ||
+ | background-image: url("https://static.igem.org/mediawiki/2015/3/3f/Aalto-Helsinki_plasmidspink.png"); | ||
+ | background-size: 100%; | ||
+ | } | ||
+ | |||
+ | </style> | ||
+ | |||
+ | |||
+ | <div class="plasmids"> | ||
+ | |||
<div class="inner-container"> | <div class="inner-container"> | ||
Line 7: | Line 23: | ||
<h2>Protocols</h2> | <h2>Protocols</h2> | ||
− | <p> | + | <p>You can find all of the protocols used in our project here as .pdf files. We based many of our protocols on what we found from research articles, openwetware.org, and other webpages. Some were given to us by 2014 Aalto-Helsinki team or researchers in Aalto University and VTT, the Techinical Research Center of Finland.</p> |
− | < | + | <h4>Preparations</h4> |
<ul> | <ul> | ||
− | <li>Chemically Competent Cells</li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/0/05/Aalto-Helsinki_chem_competent_cells.pdf" target="_blank">Chemically Competent Cells</a></p></li> |
− | <li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/9/9a/Aalto-Helsinki_chloramphenicol_plates.pdf" target="_blank">Chloramphenicol plates</a></p></li> |
− | <li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/8/88/Aalto-Helsinki_cmc_iptg_plate.pdf" target="_blank">CMC Plates with IPTG</a></p></li> |
− | <li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/0/03/Aalto-Helsinki_cmc_congo.pdf" target="_blank">CMC Plates with Congo Red</a></p></li> |
− | <li>PCR</li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/b/b5/Aalto-Helsinki_colony_pcr.pdf" target="_blank">Colony PCR</a></p></li> |
− | <li>Terrific Broth Media</li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/2/2e/Aalto-Helsinki_diluting_kit_plasmids.pdf" target="_blank">Diluting Kit Plasmids</a></p></li> |
+ | <li><p><a href="https://static.igem.org/mediawiki/2015/8/83/Aalto-Helsinki_glycerol_stock.pdf" target="_blank">Glycerol Stock of Bacteria</a></p></li> | ||
+ | <li><p><a href="https://static.igem.org/mediawiki/2015/6/6d/Aalto-Helsinki_kapa_pcr.pdf" target="_blank">PCR with KAPA</a></p></li> | ||
+ | <li><p><a href="https://static.igem.org/mediawiki/2015/a/a4/Aalto-Helsinki_q5_pcr.pdf" target="_blank">PCR with Q5</a></p></li> | ||
+ | <li><p><a href="https://static.igem.org/mediawiki/2015/4/49/Aalto-Helsinki_sds-page.pdf" target="_blank">SDS-PAGE</a></p></li> | ||
+ | <li><p><a href="https://static.igem.org/mediawiki/2015/b/b7/Aalto-Helsinki_transformation.pdf" target="_blank">Transformation</a></p></li> | ||
+ | <li><p><a href="https://static.igem.org/mediawiki/2015/4/43/Aalto-Helsinki_tetracycline_plates.pdf" target="_blank">Tetracyclin plates</a></p></li> | ||
+ | <li><p><a href="https://static.igem.org/mediawiki/2015/e/ed/Aalto-Helsinki_terrific_broth.pdf" target="_blank">Terrific Broth Media</a></p></li> | ||
+ | <li><p><a href="https://static.igem.org/mediawiki/2015/0/0a/Aalto-Helsinki_topo_cloning.pdf" target="_blank">TOPO Cloning</a></p></li> | ||
</ul> | </ul> | ||
− | < | + | <h4>Assembly</h4> |
<ul> | <ul> | ||
− | <li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/9/94/Aalto-Helsinki_elic.pdf" target="_blank">ELIC</a></p></li> |
− | <li>Ligation</li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/4/42/Aalto-Helsinki_ligation_protocol.pdf" target="_blank">Ligation</a></p></li> |
− | <li>NEBuilder</li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/7/72/Aalto-Helsinki_gibson_assembly.pdf" target="_blank">NEBuilder</a></p></li> |
− | <li>OE-PCR</li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/c/cc/Aalto-Helsinki_oe-pcr.pdf" target=_blank">OE-PCR</a></p></li> |
− | <li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/c/c0/Aalto-Helsinki_restriction_digestion.pdf" target="_blank">Restriction Digestion</a></p></li> |
</ul> | </ul> | ||
− | < | + | <h4>Measurements</h4> |
− | <ul> | + | <ul style="margin-bottom:0%; padding-bottom:8%;"> |
− | <li>Blue Chromoprotein</li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/b/b0/Aalto-Helsinki_blue_chromoprotein.pdf" target="_blank">Blue Chromoprotein</a></p></li> |
− | <li>Vial-Scale Propane Gas Chomatography</li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/4/4f/Aalto-Helsinki_cultivation_for_GC.pdf" target="_blank">Vial-Scale Propane Gas Chomatography</a></p></li> |
+ | <li><p><a href="https://static.igem.org/mediawiki/2015/a/ae/Aalto-Helsinki_purpald.pdf" target="_blank">Purpald assay</a></p></li> | ||
</ul> | </ul> | ||
− | + | <h4>Kits</h4> | |
− | <h4> | + | <ul style="margin-bottom:0%; padding-bottom:8%;"> |
− | <ul> | + | <li><p>For plasmid extraction, we used both Termo Fisher Scientific's MiniPrep kit (<a href="https://www.thermofisher.com/order/catalog/product/K0502" style="padding:0" target="_blank">#K0691</a>) and Macherey-Nagel's Plasmid Easy Pure kit (<a href="http://www.mn-net.com/ProductsBioanalysis/DNAandRNApurification/PlasmidDNApurificationeasyfastreliable/NucleoSpinPlasmidEasyPure/tabid/1384/language/en-US/Default.aspx" target="_blank">740727.250</a>).<br/> |
− | <li><a href="https:// | + | For PCR purification and gel extraction we used Thermo Fisher Scientific's kits (<a href="https://www.thermofisher.com/order/catalog/product/K0701" style="padding:0" target="_blank">#K0502</a>, <a href="https://www.thermofisher.com/order/catalog/product/K0691" style="padding:0" target="_blank">#K0691</a>)</p></li> |
− | + | ||
− | < | + | |
</ul> | </ul> | ||
− | |||
</div><!-- .inner-container --> | </div><!-- .inner-container --> | ||
+ | </div><!-- .plasmids --> | ||
</div></div></div></body> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.--> | </div></div></div></body> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.--> | ||
− | </ | + | <!-- The next starting p is to get rid of the annoying gap at the bottom of the page. There is a closing /p at some kind of ready footer, that can be seen when looking at the page source --><p style="margin:0;"> |
Latest revision as of 06:15, 17 September 2015