Difference between revisions of "Team:Aalto-Helsinki/Experiments"

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<h2>Protocols</h2>
 
<h2>Protocols</h2>
  
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<p>Describe the experiments, research and protocols you used in your iGEM project.</p>
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<p>You can find all of the protocols used in our project here as .pdf files. We based many of our protocols on what we found from research articles, openwetware.org, and other webpages. Some were given to us by 2014 Aalto-Helsinki team or researchers in Aalto University and VTT, the Techinical Research Center of Finland.</p>
  
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<h5>Preparations</h5>
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<h4>Preparations</h4>
 
<ul>
 
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<li>Chemically Competent Cells</li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/0/05/Aalto-Helsinki_chem_competent_cells.pdf" target="_blank">Chemically Competent Cells</a></p></li>
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<li>Transformation</li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/9/9a/Aalto-Helsinki_chloramphenicol_plates.pdf" target="_blank">Chloramphenicol plates</a></p></li>
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<li>Chloramphenicol plates</li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/8/88/Aalto-Helsinki_cmc_iptg_plate.pdf" target="_blank">CMC Plates with IPTG</a></p></li>
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<li>Tetracyclin plates</li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/0/03/Aalto-Helsinki_cmc_congo.pdf" target="_blank">CMC Plates with Congo Red</a></p></li>
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<li>PCR</li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/b/b5/Aalto-Helsinki_colony_pcr.pdf" target="_blank">Colony PCR</a></p></li>
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<li>Terrific Broth Media</li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/2/2e/Aalto-Helsinki_diluting_kit_plasmids.pdf" target="_blank">Diluting Kit Plasmids</a></p></li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/8/83/Aalto-Helsinki_glycerol_stock.pdf" target="_blank">Glycerol Stock of Bacteria</a></p></li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/6/6d/Aalto-Helsinki_kapa_pcr.pdf" target="_blank">PCR with KAPA</a></p></li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/a/a4/Aalto-Helsinki_q5_pcr.pdf" target="_blank">PCR with Q5</a></p></li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/4/49/Aalto-Helsinki_sds-page.pdf" target="_blank">SDS-PAGE</a></p></li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/b/b7/Aalto-Helsinki_transformation.pdf" target="_blank">Transformation</a></p></li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/4/43/Aalto-Helsinki_tetracycline_plates.pdf" target="_blank">Tetracyclin plates</a></p></li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/e/ed/Aalto-Helsinki_terrific_broth.pdf" target="_blank">Terrific Broth Media</a></p></li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/0/0a/Aalto-Helsinki_topo_cloning.pdf" target="_blank">TOPO Cloning</a></p></li>
 
</ul>
 
</ul>
  
  
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<h5>Assembly</h5>
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<h4>Assembly</h4>
 
<ul>
 
<ul>
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<li>Restriction</li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/9/94/Aalto-Helsinki_elic.pdf" target="_blank">ELIC</a></p></li>
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<li>Ligation</li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/4/42/Aalto-Helsinki_ligation_protocol.pdf" target="_blank">Ligation</a></p></li>
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<li>NEBuilder</li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/7/72/Aalto-Helsinki_gibson_assembly.pdf" target="_blank">NEBuilder</a></p></li>
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<li>OE-PCR</li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/c/cc/Aalto-Helsinki_oe-pcr.pdf" target=_blank">OE-PCR</a></p></li>
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<li>ELIC</li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/c/c0/Aalto-Helsinki_restriction_digestion.pdf" target="_blank">Restriction Digestion</a></p></li>
 
</ul>
 
</ul>
  
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<h5>Measurements</h5>
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<h4>Measurements</h4>
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<ul>
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<ul style="margin-bottom:0%; padding-bottom:8%;">
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<li>Blue Chromoprotein</li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/b/b0/Aalto-Helsinki_blue_chromoprotein.pdf" target="_blank">Blue Chromoprotein</a></p></li>
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<li>Vial-Scale Propane Gas Chomatography</li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/4/4f/Aalto-Helsinki_cultivation_for_GC.pdf" target="_blank">Vial-Scale Propane Gas Chomatography</a></p></li>
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<li><p><a href="https://static.igem.org/mediawiki/2015/a/ae/Aalto-Helsinki_purpald.pdf" target="_blank">Purpald assay</a></p></li>
 
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</ul>
  
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<h4>Kits</h4>
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<h4>Inspiration</h4>
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<ul style="margin-bottom:0%; padding-bottom:8%;">
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<ul>
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<li><p>For plasmid extraction, we used both Termo Fisher Scientific's MiniPrep kit (<a href="https://www.thermofisher.com/order/catalog/product/K0502" style="padding:0" target="_blank">#K0691</a>) and Macherey-Nagel's Plasmid Easy Pure kit (<a href="http://www.mn-net.com/ProductsBioanalysis/DNAandRNApurification/PlasmidDNApurificationeasyfastreliable/NucleoSpinPlasmidEasyPure/tabid/1384/language/en-US/Default.aspx" target="_blank">740727.250</a>).<br/>
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<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
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For PCR purification and gel extraction we used Thermo Fisher Scientific's kits (<a href="https://www.thermofisher.com/order/catalog/product/K0701" style="padding:0" target="_blank">#K0502</a>, <a href="https://www.thermofisher.com/order/catalog/product/K0691" style="padding:0" target="_blank">#K0691</a>)</p></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
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Latest revision as of 06:15, 17 September 2015

Protocols

You can find all of the protocols used in our project here as .pdf files. We based many of our protocols on what we found from research articles, openwetware.org, and other webpages. Some were given to us by 2014 Aalto-Helsinki team or researchers in Aalto University and VTT, the Techinical Research Center of Finland.

Preparations

Assembly

Measurements

Kits

  • For plasmid extraction, we used both Termo Fisher Scientific's MiniPrep kit (#K0691) and Macherey-Nagel's Plasmid Easy Pure kit (740727.250).
    For PCR purification and gel extraction we used Thermo Fisher Scientific's kits (#K0502, #K0691)