Difference between revisions of "Team:Aalto-Helsinki/Experiments"
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<li><p><a href="https://static.igem.org/mediawiki/2015/0/05/Aalto-Helsinki_chem_competent_cells.pdf" target="_blank">Chemically Competent Cells</a></p></li> | <li><p><a href="https://static.igem.org/mediawiki/2015/0/05/Aalto-Helsinki_chem_competent_cells.pdf" target="_blank">Chemically Competent Cells</a></p></li> | ||
<li><p><a href="https://static.igem.org/mediawiki/2015/9/9a/Aalto-Helsinki_chloramphenicol_plates.pdf" target="_blank">Chloramphenicol plates</a></p></li> | <li><p><a href="https://static.igem.org/mediawiki/2015/9/9a/Aalto-Helsinki_chloramphenicol_plates.pdf" target="_blank">Chloramphenicol plates</a></p></li> | ||
| − | <li><p><a href"https://static.igem.org/mediawiki/2015/8/88/Aalto-Helsinki_cmc_iptg_plate.pdf" target="_blank">CMC Plates with IPTG</a></p></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/8/88/Aalto-Helsinki_cmc_iptg_plate.pdf" target="_blank">CMC Plates with IPTG</a></p></li> |
<li><p><a href="https://static.igem.org/mediawiki/2015/0/03/Aalto-Helsinki_cmc_congo.pdf" target="_blank">CMC Plates with Congo Red</a></p></li> | <li><p><a href="https://static.igem.org/mediawiki/2015/0/03/Aalto-Helsinki_cmc_congo.pdf" target="_blank">CMC Plates with Congo Red</a></p></li> | ||
<li><p><a href="https://static.igem.org/mediawiki/2015/b/b5/Aalto-Helsinki_colony_pcr.pdf" target="_blank">Colony PCR</a></p></li> | <li><p><a href="https://static.igem.org/mediawiki/2015/b/b5/Aalto-Helsinki_colony_pcr.pdf" target="_blank">Colony PCR</a></p></li> | ||
| + | <li><p><a href="https://static.igem.org/mediawiki/2015/2/2e/Aalto-Helsinki_diluting_kit_plasmids.pdf" target="_blank">Diluting Kit Plasmids</a></p></li> | ||
<li><p><a href="https://static.igem.org/mediawiki/2015/8/83/Aalto-Helsinki_glycerol_stock.pdf" target="_blank">Glycerol Stock of Bacteria</a></p></li> | <li><p><a href="https://static.igem.org/mediawiki/2015/8/83/Aalto-Helsinki_glycerol_stock.pdf" target="_blank">Glycerol Stock of Bacteria</a></p></li> | ||
| − | <li><p><a href="https:// | + | <li><p><a href="https://static.igem.org/mediawiki/2015/6/6d/Aalto-Helsinki_kapa_pcr.pdf" target="_blank">PCR with KAPA</a></p></li> |
<li><p><a href="https://static.igem.org/mediawiki/2015/a/a4/Aalto-Helsinki_q5_pcr.pdf" target="_blank">PCR with Q5</a></p></li> | <li><p><a href="https://static.igem.org/mediawiki/2015/a/a4/Aalto-Helsinki_q5_pcr.pdf" target="_blank">PCR with Q5</a></p></li> | ||
| − | <li><p><a href="https://static.igem.org/mediawiki/2015/ | + | <li><p><a href="https://static.igem.org/mediawiki/2015/4/49/Aalto-Helsinki_sds-page.pdf" target="_blank">SDS-PAGE</a></p></li> |
<li><p><a href="https://static.igem.org/mediawiki/2015/b/b7/Aalto-Helsinki_transformation.pdf" target="_blank">Transformation</a></p></li> | <li><p><a href="https://static.igem.org/mediawiki/2015/b/b7/Aalto-Helsinki_transformation.pdf" target="_blank">Transformation</a></p></li> | ||
| − | <li><p>Tetracyclin plates</p></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/4/43/Aalto-Helsinki_tetracycline_plates.pdf" target="_blank">Tetracyclin plates</a></p></li> |
<li><p><a href="https://static.igem.org/mediawiki/2015/e/ed/Aalto-Helsinki_terrific_broth.pdf" target="_blank">Terrific Broth Media</a></p></li> | <li><p><a href="https://static.igem.org/mediawiki/2015/e/ed/Aalto-Helsinki_terrific_broth.pdf" target="_blank">Terrific Broth Media</a></p></li> | ||
<li><p><a href="https://static.igem.org/mediawiki/2015/0/0a/Aalto-Helsinki_topo_cloning.pdf" target="_blank">TOPO Cloning</a></p></li> | <li><p><a href="https://static.igem.org/mediawiki/2015/0/0a/Aalto-Helsinki_topo_cloning.pdf" target="_blank">TOPO Cloning</a></p></li> | ||
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<h4>Assembly</h4> | <h4>Assembly</h4> | ||
<ul> | <ul> | ||
| − | <li><p>ELIC</p></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/9/94/Aalto-Helsinki_elic.pdf" target="_blank">ELIC</a></p></li> |
<li><p><a href="https://static.igem.org/mediawiki/2015/4/42/Aalto-Helsinki_ligation_protocol.pdf" target="_blank">Ligation</a></p></li> | <li><p><a href="https://static.igem.org/mediawiki/2015/4/42/Aalto-Helsinki_ligation_protocol.pdf" target="_blank">Ligation</a></p></li> | ||
<li><p><a href="https://static.igem.org/mediawiki/2015/7/72/Aalto-Helsinki_gibson_assembly.pdf" target="_blank">NEBuilder</a></p></li> | <li><p><a href="https://static.igem.org/mediawiki/2015/7/72/Aalto-Helsinki_gibson_assembly.pdf" target="_blank">NEBuilder</a></p></li> | ||
| − | <li><p>OE-PCR</p></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/c/cc/Aalto-Helsinki_oe-pcr.pdf" target=_blank">OE-PCR</a></p></li> |
| − | <li><p><a href="https://static.igem.org/mediawiki/2015/c/c0/Aalto-Helsinki_restriction_digestion.pdf" target="_blank">Restriction</a></p></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/c/c0/Aalto-Helsinki_restriction_digestion.pdf" target="_blank">Restriction Digestion</a></p></li> |
</ul> | </ul> | ||
<h4>Measurements</h4> | <h4>Measurements</h4> | ||
| − | <ul style="margin-bottom:0%; padding-bottom:8%;> | + | <ul style="margin-bottom:0%; padding-bottom:8%;"> |
<li><p><a href="https://static.igem.org/mediawiki/2015/b/b0/Aalto-Helsinki_blue_chromoprotein.pdf" target="_blank">Blue Chromoprotein</a></p></li> | <li><p><a href="https://static.igem.org/mediawiki/2015/b/b0/Aalto-Helsinki_blue_chromoprotein.pdf" target="_blank">Blue Chromoprotein</a></p></li> | ||
<li><p><a href="https://static.igem.org/mediawiki/2015/4/4f/Aalto-Helsinki_cultivation_for_GC.pdf" target="_blank">Vial-Scale Propane Gas Chomatography</a></p></li> | <li><p><a href="https://static.igem.org/mediawiki/2015/4/4f/Aalto-Helsinki_cultivation_for_GC.pdf" target="_blank">Vial-Scale Propane Gas Chomatography</a></p></li> | ||
| + | <li><p><a href="https://static.igem.org/mediawiki/2015/a/ae/Aalto-Helsinki_purpald.pdf" target="_blank">Purpald assay</a></p></li> | ||
</ul> | </ul> | ||
| + | <h4>Kits</h4> | ||
| + | <ul style="margin-bottom:0%; padding-bottom:8%;"> | ||
| + | <li><p>For plasmid extraction, we used both Termo Fisher Scientific's MiniPrep kit (<a href="https://www.thermofisher.com/order/catalog/product/K0502" style="padding:0" target="_blank">#K0691</a>) and Macherey-Nagel's Plasmid Easy Pure kit (<a href="http://www.mn-net.com/ProductsBioanalysis/DNAandRNApurification/PlasmidDNApurificationeasyfastreliable/NucleoSpinPlasmidEasyPure/tabid/1384/language/en-US/Default.aspx" target="_blank">740727.250</a>).<br/> | ||
| + | For PCR purification and gel extraction we used Thermo Fisher Scientific's kits (<a href="https://www.thermofisher.com/order/catalog/product/K0701" style="padding:0" target="_blank">#K0502</a>, <a href="https://www.thermofisher.com/order/catalog/product/K0691" style="padding:0" target="_blank">#K0691</a>)</p></li> | ||
| + | </ul> | ||
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Latest revision as of 06:15, 17 September 2015
