Difference between revisions of "Team:TU Dresden/Notebook/PlasmidDesign"

 
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<h3>Plasmid Maps</h3>
 
<h3>Plasmid Maps</h3>
 
<h4>M13 gene 3 coding for infection protein P3</h4>
 
<h4>M13 gene 3 coding for infection protein P3</h4>
For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/b/b3/TU_Dresden_geneIII_first.pdf"><font color="#045FB4">here</font></a>
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    <figure align="center">
<figure align="center">
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    <embed src="https://static.igem.org/mediawiki/2015/b/b3/TU_Dresden_geneIII_first.pdf" width="800" height="450" type='application/pdf'>
<a href="https://static.igem.org/mediawiki/2015/e/e8/TU_Dresden_geneIII_first_cut.jpg" title="View">
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    </figure>
  <IMG SRC="https://static.igem.org/mediawiki/2015/e/e8/TU_Dresden_geneIII_first_cut.jpg" style="width: 50%;"/></a>
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</figure>
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<h4>HER2 (Human Epidermal Growth Factor 2) cytoplasmic domain</h4>
 
<h4>HER2 (Human Epidermal Growth Factor 2) cytoplasmic domain</h4>
For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/3/3a/TU_Dresden_her2_first.pdf"><font color="#045FB4">here</font></a>
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    <figure align="center">
<figure align="center">
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    <embed src="https://static.igem.org/mediawiki/2015/3/3a/TU_Dresden_her2_first.pdf" width="800" height="450" type='application/pdf'>
<a href="https://static.igem.org/mediawiki/2015/3/3a/TU_Dresden_her2_first.pdf" title="View">
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    </figure>
  <IMG SRC="https://static.igem.org/mediawiki/2015/b/b7/TU_Dresden_her2_first_cut.jpg" style="width: 50%;"/></a>
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</figure>
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<h4>ZHER2 - affibody with affinity to HER2</h4>
 
<h4>ZHER2 - affibody with affinity to HER2</h4>
For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/d/d9/TU_Dresden_zher2_first.pdf"><font color="#045FB4">here</font></a>
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    <figure align="center">
<figure align="center">
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    <embed src="https://static.igem.org/mediawiki/2015/d/d9/TU_Dresden_zher2_first.pdf" width="800" height="450" type='application/pdf'>
<a href="https://static.igem.org/mediawiki/2015/e/e0/TU_Dresden_zher2_affibody_first_cut.jpg" title="View">
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    </figure>
  <IMG SRC="https://static.igem.org/mediawiki/2015/e/e0/TU_Dresden_zher2_affibody_first_cut.jpg" style="width: 50%;"/></a>
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</figure>
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<h4>T25 - subunit of BACTH system</h4>
 
<h4>T25 - subunit of BACTH system</h4>
For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/2/26/TU_Dresden_t25_first.pdf"><font color="#045FB4">here</font></a>
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    <figure align="center">
<figure align="center">
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    <embed src="https://static.igem.org/mediawiki/2015/2/26/TU_Dresden_t25_first.pdf" width="800" height="450" type='application/pdf'>
<a href="https://static.igem.org/mediawiki/2015/7/75/TU_Dresden_t25_bacth_first_cut.jpg" title="View">
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    </figure>
  <IMG SRC="https://static.igem.org/mediawiki/2015/7/75/TU_Dresden_t25_bacth_first_cut.jpg" style="width: 50%;"/></a>
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</figure>
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<h4>T18 - subunit of BACTH system</h4>
 
<h4>T18 - subunit of BACTH system</h4>
For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/3/38/TU_Dresden_t18_first.pdf"><font color="#045FB4">here</font></a>
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    <figure align="center">
<figure align="center">
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    <embed src="https://static.igem.org/mediawiki/2015/3/38/TU_Dresden_t18_first.pdf" width="800" height="450" type='application/pdf'>
<a href="https://static.igem.org/mediawiki/2015/0/0b/TU_Dresden_t18_bacth_first_cut.jpg" title="View">
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    </figure>
  <IMG SRC="https://static.igem.org/mediawiki/2015/0/0b/TU_Dresden_t18_bacth_first_cut.jpg" style="width: 50%;"/></a>
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</figure>
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<h4>Leucine zipper able to bind to T25 BACTH subunit</h4>
 
<h4>Leucine zipper able to bind to T25 BACTH subunit</h4>
For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/5/57/TU_Dresden_lzt25_first.pdf"><font color="#045FB4">here</font></a>
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    <figure align="center">
<figure align="center">
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    <embed src="https://static.igem.org/mediawiki/2015/5/57/TU_Dresden_lzt25_first.pdf" width="800" height="450" type='application/pdf'>
<a href="https://static.igem.org/mediawiki/2015/a/a3/TU_Dresden_lzt25_bacth_first_cut.jpg" title="View">
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    </figure>
  <IMG SRC="https://static.igem.org/mediawiki/2015/a/a3/TU_Dresden_lzt25_bacth_first_cut.jpg" style="width: 50%;"/></a>
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<h4>Leucine zipper able to bind to T18 BACTH subunit</h4>
</figure>
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    <figure align="center">
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    <embed src="https://static.igem.org/mediawiki/2015/1/1f/TU_Dresden_lzt18_first.pdf" width="800" height="450" type='application/pdf'>
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    </figure>
  
<h4>Leucine zipper able to bind to T18 BACTH subunit</h4>
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<p></p>
For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/1/1f/TU_Dresden_lzt18_first.pdf"><font color="#045FB4">here</font></a>
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<h3 style="text-align:center;"><a style="text-decoration:none;" href="#top"><font color="#045FB4">To the top!</font></a></h3>
<figure align="center">
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<a href="https://static.igem.org/mediawiki/2015/c/cd/TU_Dresden_lzt18_bacth_first_cut.jpg" title="View">
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   <IMG SRC="https://static.igem.org/mediawiki/2015/c/cd/TU_Dresden_lzt18_bacth_first_cut.jpg" style="width: 50%;"/></a>
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Latest revision as of 07:54, 17 September 2015


Plasmid Design

All plasmids designed in this section were used as parts for the various subprojects. These plasmids were initially designed to have kanomycin selection marker. These were synthesized from IDT. Below, the plasmid maps of the various parts characterized as for this project can be found.

Please refer to the Parts section for the final updated parts in iGEM Biobrick format.

Plasmid Maps

M13 gene 3 coding for infection protein P3

HER2 (Human Epidermal Growth Factor 2) cytoplasmic domain

ZHER2 - affibody with affinity to HER2

T25 - subunit of BACTH system

T18 - subunit of BACTH system

Leucine zipper able to bind to T25 BACTH subunit

Leucine zipper able to bind to T18 BACTH subunit

To the top!