Difference between revisions of "Team:Technion Israel/Lab Notebook/Protocols"
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<ul> | <ul> | ||
<li style="list-style-type: none;"> | <li style="list-style-type: none;"> | ||
| − | < | + | <a href="https://static.igem.org/mediawiki/2015/b/b8/Techion_Israel_2015_protocols_gelpreparation.pdf" target="_blank">Gel Preparation</a> |
</li> | </li> | ||
<li style="list-style-type: none;"> | <li style="list-style-type: none;"> | ||
| − | < | + | <a href="https://static.igem.org/mediawiki/2015/b/ba/Technion_Israel_2015_protocols_miniprep.pdf" target="_blank">Sigma-Aldrich Miniprep</a> |
</li> | </li> | ||
<li style="list-style-type: none;"> | <li style="list-style-type: none;"> | ||
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</ol> | </ol> | ||
<ol> | <ol> | ||
| − | <a href="https://static.igem.org/mediawiki/2015/e/ef/Technion_iGEM_2015_B.Subtilis_Transformation.pdf"><h3><i>B.Subtilis</i></h3></a> | + | <a href="https://static.igem.org/mediawiki/2015/e/ef/Technion_iGEM_2015_B.Subtilis_Transformation.pdf" target="_blank"><h3><i>B.Subtilis</i></h3></a> |
</ol> | </ol> | ||
</li> | </li> | ||
| Line 364: | Line 364: | ||
<li style="list-style-type: none;"> | <li style="list-style-type: none;"> | ||
<h2>Colony PCR</h2> | <h2>Colony PCR</h2> | ||
| − | <ol | + | <ol><a href="https://static.igem.org/mediawiki/2015/7/7c/Technion_Israel_2015_protocols_colonyPCR.pdf" target="_blank"><h3><i>E. coli</i></h3></a></ol> |
| − | < | + | <ol><a href="https://static.igem.org/mediawiki/2015/b/bf/Technion_Israel_2015_Colony_PCR_for_B.Subtilis_.pdf" target="_blank"><h3><i>B. Subtilis</i></h3></a> |
| + | |||
</ol> | </ol> | ||
</li> | </li> | ||
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<a href="https://static.igem.org/mediawiki/2015/4/4b/Technion_Israel_2015_LysisMethodComparison.pdf" target="_blank"><h2>Comparison of Lysis Procedures</h2></a> | <a href="https://static.igem.org/mediawiki/2015/4/4b/Technion_Israel_2015_LysisMethodComparison.pdf" target="_blank"><h2>Comparison of Lysis Procedures</h2></a> | ||
<a href="https://static.igem.org/mediawiki/2015/e/e2/Technion_Israel_2015_protocols_B.subtilis-mCherry-secretion.pdf" target="_blank"><h2>Bacillus Secretion Verification</h2></a> | <a href="https://static.igem.org/mediawiki/2015/e/e2/Technion_Israel_2015_protocols_B.subtilis-mCherry-secretion.pdf" target="_blank"><h2>Bacillus Secretion Verification</h2></a> | ||
| + | <a href="https://static.igem.org/mediawiki/2015/7/7e/Technion_Israel_2015_protocols_3aHSDactivity.pdf" target="_blank"><h2>3ɑ-HSD Activity Verification Protocol</h2></a> | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 08:51, 17 September 2015
Protocols
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- Gel Preparation
- Sigma-Aldrich Miniprep
-
Phusion PCR
-
Electroporation
E.coli
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Transformation
E.coli
B.Subtilis
-
Colony PCR
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Preparing Competent E.coli
-
DNA Gel Electrophoresis
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Preparation of DNA for Sequencing
