Difference between revisions of "Team:CGU Taiwan/Notebook"
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+ | <div class="single-blog blog-details two-column" id="Protocols"> | ||
+ | <h2 class="post-title bold">GPCR</h2> | ||
+ | </div> | ||
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− | + | Operator: Wan yun, Jinting , Jinye | |
− | + | Goal: | |
− | + | 1. Extraction of gDNA of Far1∆::KANMX strain | |
− | + | 2. Detection the concentration of fast extracted gDNA of ∆Far1 strain | |
− | + | 3. PCR gDNA of Far1∆ ::KANMX | |
− | + | 4. Electrophoresis to check PCR product | |
− | + | Experiment steps: | |
− | + | < Extraction of gDNA of Far1∆::KANMX strain> | |
− | + | Consult the protocol<protocol of fast extraction of gDNA of yeast> | |
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− | + | <Detection the concentration fo fast extracted gDNA> | |
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<table class="protocol-table"> | <table class="protocol-table"> | ||
<thead> | <thead> | ||
<tr><th></th><th></th></tr> | <tr><th></th><th></th></tr> | ||
</thead> | </thead> | ||
− | <tr><td> </td><td> </td | + | <tr><td>Strains </td><td>260/280 </td><td>260/230 </td><td>C(ng/μl) </td></tr> |
− | + | <tr><td>Far1∆::KANMX strain </td><td>1.62 </td><td>0.97 </td><td>44.7 </td></tr> | |
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− | <tr><td> </td><td> </td | + | |
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</table> | </table> | ||
+ | <PCR gDNA of Far1∆::KANMX > | ||
+ | 1. Design of primers | ||
<table class="protocol-table"> | <table class="protocol-table"> | ||
<thead> | <thead> | ||
<tr><th></th><th></th></tr> | <tr><th></th><th></th></tr> | ||
</thead> | </thead> | ||
− | <tr><td> </td><td> </td></ | + | <tr><td> </td><td> </td><td> </td><td> </td><td> </td><td>Tm (℃)</td><td>GC (%)</td><td>Nmole</td><td>μl for 100μM </td></tr> |
− | + | <tr><td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td>55</td><td>21.25</td><td>212.50</td></tr> | |
− | <tr><td> </td><td> </td></ | + | <tr><td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td>41</td><td>17.12</td><td>171.20</td></tr> |
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</table> | </table> | ||
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</section> | </section> |
Revision as of 11:56, 17 September 2015
GPCR
Operator: Wan yun, Jinting , Jinye
Goal:
1. Extraction of gDNA of Far1∆::KANMX strain
2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
3. PCR gDNA of Far1∆ ::KANMX
4. Electrophoresis to check PCR product
Experiment steps:
< Extraction of gDNA of Far1∆::KANMX strain>
Consult the protocol
1. Design of primers
Strains | 260/280 | 260/230 | C(ng/μl) |
Far1∆::KANMX strain | 1.62 | 0.97 | 44.7 |
Tm (℃) | GC (%) | Nmole | μl for 100μM | |||||
55 | 21.25 | 212.50 | ||||||
41 | 17.12 | 171.20 |
GPCR
Operator: Wan yun, Jinting , Jinye
Goal:
1. Extraction of gDNA of Far1∆::KANMX strain
2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
3. PCR gDNA of Far1∆ ::KANMX
4. Electrophoresis to check PCR product
Experiment steps:
< Extraction of gDNA of Far1∆::KANMX strain>
Consult the protocol
1. Design of primers
Strains | 260/280 | 260/230 | C(ng/μl) |
Far1∆::KANMX strain | 1.62 | 0.97 | 44.7 |
Tm (℃) | GC (%) | Nmole | μl for 100μM | |||||
55 | 21.25 | 212.50 | ||||||
41 | 17.12 | 171.20 |