Difference between revisions of "Team:CGU Taiwan/Notebook"

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<div class="single-blog blog-details two-column" id="Protocols">
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<h2 class="post-title bold">GPCR</h2>
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Operator: Wan yun, Jinting , Jinye
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Goal:
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1. Extraction of gDNA of Far1∆::KANMX strain
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    2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
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3. PCR gDNA of Far1∆ ::KANMX
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4. Electrophoresis to check PCR product
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Experiment steps:
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< Extraction of gDNA of Far1∆::KANMX strain>
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Consult the protocol<protocol of fast extraction of gDNA of yeast>
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<Detection the concentration fo fast extracted gDNA>
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<tr><td>Strains </td><td>260/280 </td><td>260/230 </td><td>C(ng/μl) </td></tr>
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<tr><td>Far1∆::KANMX strain </td><td>1.62 </td><td>0.97 </td><td>44.7 </td></tr>
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<PCR gDNA of Far1∆::KANMX >
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1. Design of primers
 
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<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td>Tm (℃)</td><td>GC (%)</td><td>Nmole</td><td>μl for 100μM </td></tr>
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<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td>55</td><td>21.25</td><td>212.50</td></tr>
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<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td>41</td><td>17.12</td><td>171.20</td></tr>
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Revision as of 11:56, 17 September 2015

Home | CGU_Taiwan

Home | CGU_Taiwan

GPCR

Operator: Wan yun, Jinting , Jinye Goal: 1. Extraction of gDNA of Far1∆::KANMX strain 2. Detection the concentration of fast extracted gDNA of ∆Far1 strain 3. PCR gDNA of Far1∆ ::KANMX 4. Electrophoresis to check PCR product Experiment steps: < Extraction of gDNA of Far1∆::KANMX strain> Consult the protocol
Strains 260/280 260/230 C(ng/μl)
Far1∆::KANMX strain 1.62 0.97 44.7
1. Design of primers
Tm (℃)GC (%)Nmoleμl for 100μM
5521.25212.50
4117.12171.20

GPCR

Operator: Wan yun, Jinting , Jinye Goal: 1. Extraction of gDNA of Far1∆::KANMX strain 2. Detection the concentration of fast extracted gDNA of ∆Far1 strain 3. PCR gDNA of Far1∆ ::KANMX 4. Electrophoresis to check PCR product Experiment steps: < Extraction of gDNA of Far1∆::KANMX strain> Consult the protocol
Strains 260/280 260/230 C(ng/μl)
Far1∆::KANMX strain 1.62 0.97 44.7
1. Design of primers
Tm (℃)GC (%)Nmoleμl for 100μM
5521.25212.50
4117.12171.20