Difference between revisions of "Team:Bordeaux/Yeast results"

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         <h5 align="center"><b>CHOICE OF MATERIALS</b></h5>
 
         <h5 align="center"><b>CHOICE OF MATERIALS</b></h5>
 
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<h6 align="justify"><i>FKS1</i> gene</h6
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<p align=justify>&#10037; <b><i>FKS1</i>gene</b> codes a D-glucan synthase. </p>
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<h6 align="justify"><i>pYES2</i> plasmid</h6>
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<p align="justify">The plasmid used is the <i>pYES2</i> plasmid provided by <b>M. Didier Thoraval</b> (membrane biogenesis laboratory). It contains an inducible promoter to galactose (<i>GAL1</i>) and a selection gene <i>URA3</i>.</p>
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<h6 align="justify"><i>Saccharomyces cerevisiae</i> strain</h6>
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<p align="justify"> All results were obtained with haploid Lnvsc1 strain of Saccharomyces cerevisiae. Its genotype isLeu / Trp / Ura- / Hist- mat α.
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This genotype allows us an easy selection of transformed cells. </p>
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Revision as of 12:10, 17 September 2015

IGEM Bordeaux 2015





CHOICE OF MATERIALS

FKS1 gene
✵ FKS1gene codes a D-glucan synthase.

pYES2 plasmid

The plasmid used is the pYES2 plasmid provided by M. Didier Thoraval (membrane biogenesis laboratory). It contains an inducible promoter to galactose (GAL1) and a selection gene URA3.

Saccharomyces cerevisiae strain

All results were obtained with haploid Lnvsc1 strain of Saccharomyces cerevisiae. Its genotype isLeu / Trp / Ura- / Hist- mat α. This genotype allows us an easy selection of transformed cells.