Difference between revisions of "Team:Bordeaux/Yeast results"

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<h6 align="justify"><i>FKS1</i> gene</h6  
 
<h6 align="justify"><i>FKS1</i> gene</h6  
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<p align=justify>&#10037; <i>FKS1</i> gene codes a D-glucan synthase. </p>
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<p align=justify> <i>FKS1</i> gene codes a D-glucan synthase. </p>
 
                            
 
                            
 
<h6 align="justify"><i>pYES2</i> plasmid</h6>
 
<h6 align="justify"><i>pYES2</i> plasmid</h6>

Revision as of 12:12, 17 September 2015

IGEM Bordeaux 2015





CHOICE OF MATERIALS

FKS1 gene
FKS1 gene codes a D-glucan synthase.

pYES2 plasmid

The plasmid used is the pYES2 plasmid provided by M. Didier Thoraval (membrane biogenesis laboratory). It contains an inducible promoter to galactose (GAL1) and a selection gene URA3.

Saccharomyces cerevisiae strain

All results were obtained with haploid Lnvsc1 strain of Saccharomyces cerevisiae. Its genotype isLeu / Trp / Ura- / Hist- mat α. This genotype allows us an easy selection of transformed cells.

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