Difference between revisions of "Team:Uppsala/Experiments"

Line 3: Line 3:
 
<head>
 
<head>
 
<style>
 
<style>
 +
#prot_list {
 +
list-style: decimal;
 +
font-family: "Trebuchet MS"
 +
}
 +
 +
#prot_list span {
 +
font-family: "Trebuchet MS";
 +
}
  
 
</style>
 
</style>
Line 10: Line 18:
  
 
   <h1>Experiments and protocols</h1>
 
   <h1>Experiments and protocols</h1>
 +
  <p>
 +
  A majority of the protocols we used originate from the book "Synthetic biology-A lab manual", written by Anthony Forster, Erik Gullberg and Josefine Liljeruhm. They can therefore not be published here. The following protocols from the sixth chapter of this book were used:
 +
  </p>
 +
  <ol id="prot_list">
 +
  <li><span>0.9% NaCl</span></li>
 +
  <li><span>50% Glycerol</span></li>
 +
  <li><span>1M CaCl<sub>2</sub></span></li>
 +
  <li><span>10x TBE Buffer</span></li>
 +
  <li><span>SOB Medium</span></li>
 +
  <li><span>LB Medium</span></li>
 +
  <li><span>LB Agar Plates and Addition of Antibiotics</span></li>
 +
  <li><span>Overnight Cultures with antibiotics, and glycerol stocks</span></li>
 +
  <li><span>Agarose gel electrophoresis</span></li>
 +
  <li><span>Preparation of competent E. coli cells using CaCl<sub>2</sub></span></li>
 +
  <li><span>Transformation of CaCl<sub>2</sub> competent E. coli cells</span></li>
 +
  <li><span>Bacterial re-streak techniques</span></li>
 +
  <li><span>Digestion with DpnI</span></li>
 +
  <li><span>3A assembly</span></li>
 +
  <li><span>Colony PCR</span></li>
 +
  </ol>
 +
  <p>We also used several other protocols which we share below.</p>
  
 
  </div>
 
  </div>

Revision as of 12:17, 17 September 2015

Experiments and protocols

A majority of the protocols we used originate from the book "Synthetic biology-A lab manual", written by Anthony Forster, Erik Gullberg and Josefine Liljeruhm. They can therefore not be published here. The following protocols from the sixth chapter of this book were used:

  1. 0.9% NaCl
  2. 50% Glycerol
  3. 1M CaCl2
  4. 10x TBE Buffer
  5. SOB Medium
  6. LB Medium
  7. LB Agar Plates and Addition of Antibiotics
  8. Overnight Cultures with antibiotics, and glycerol stocks
  9. Agarose gel electrophoresis
  10. Preparation of competent E. coli cells using CaCl2
  11. Transformation of CaCl2 competent E. coli cells
  12. Bacterial re-streak techniques
  13. Digestion with DpnI
  14. 3A assembly
  15. Colony PCR

We also used several other protocols which we share below.