Difference between revisions of "Team:LZU-China/Parts"
Ritianjiang (Talk | contribs) |
Ritianjiang (Talk | contribs) |
||
| Line 92: | Line 92: | ||
<div> | <div> | ||
<strong><h6><p>RibB is our basic gene. Every part basic on it.</p></h6></strong> | <strong><h6><p>RibB is our basic gene. Every part basic on it.</p></h6></strong> | ||
| + | |||
| + | <div class="row"> | ||
| + | <p> <div class="col s12 m5 l5"> | ||
| + | <div class="card"> | ||
| + | <div class="card-image"> | ||
| + | <img class="left-align" src="https://static.igem.org/mediawiki/parts/5/5f/Constitutive.png"> | ||
| + | |||
| + | |||
| + | <span class="card-title"></span> | ||
| + | </div> | ||
| + | <div class="card-content"> | ||
| + | <p>Figure 1:Constitutive parts <br/>K1755005 K1755006 K1755007 K1755008 and K1755009</p> | ||
| + | </div> | ||
| + | <!--<div class="card-action"> | ||
| + | <a href="#">This is a link</a> | ||
| + | </div>--> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | <div class="col s12 m5 l5"> | ||
| + | <div class="card"> | ||
| + | <div class="card-image"> | ||
| + | <img class="right-align" src="https://static.igem.org/mediawiki/parts/thumb/6/6e/IGEM_2015_MFC.JPG/600px-IGEM_2015_MFC.JPG"> | ||
| + | <span class="card-title"></span> | ||
| + | </div> | ||
| + | <div class="card-content"> | ||
| + | <p>Figure 2:Our parts working in MFC</p> | ||
| + | </div> | ||
| + | <!--<div class="card-action"> | ||
| + | <a href="#">This is a link</a> | ||
| + | </div>--> | ||
| + | </div> | ||
| + | </div></p> | ||
| + | </div> | ||
<p> | <p> | ||
The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate. | The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate. | ||
| Line 103: | Line 137: | ||
<div class="card"> | <div class="card"> | ||
<div class="card-image"> | <div class="card-image"> | ||
| − | <img class="left-align" src="https://static.igem.org/mediawiki/parts/ | + | <img class="left-align" src="https://static.igem.org/mediawiki/parts/0/04/K1755301ceshi.png"> |
| Line 109: | Line 143: | ||
</div> | </div> | ||
<div class="card-content"> | <div class="card-content"> | ||
| − | <p>Figure | + | <p>Figure 3:Concentration of riboflavin vs Cu ions</p> |
</div> | </div> | ||
<!--<div class="card-action"> | <!--<div class="card-action"> | ||
| Line 120: | Line 154: | ||
<div class="card"> | <div class="card"> | ||
<div class="card-image"> | <div class="card-image"> | ||
| − | <img class="right-align" src="https://static.igem.org/mediawiki/parts/ | + | <img class="right-align" src="https://static.igem.org/mediawiki/parts/b/b5/K1755301time.png"> |
<span class="card-title"></span> | <span class="card-title"></span> | ||
</div> | </div> | ||
<div class="card-content"> | <div class="card-content"> | ||
| − | <p>Figure | + | <p>Figure 4:Riboflavin concentration when the CuSO<sub>4</sub> in 5mg/L</p> |
</div> | </div> | ||
<!--<div class="card-action"> | <!--<div class="card-action"> | ||
| Line 130: | Line 164: | ||
</div>--> | </div>--> | ||
</div> | </div> | ||
| − | + | </div></p> | |
| − | + | </div> | |
<P> We construct many parts as the following list.</P> | <P> We construct many parts as the following list.</P> | ||
Revision as of 12:22, 17 September 2015
RibB is our basic gene. Every part basic on it.
Figure 1:Constitutive parts
K1755005 K1755006 K1755007 K1755008 and K1755009
Figure 2:Our parts working in MFC
The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate. This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate. Proteins known to be involved in this subpathway in this organism are: 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
Figure 3:Concentration of riboflavin vs Cu ions
Figure 4:Riboflavin concentration when the CuSO4 in 5mg/L
We construct many parts as the following list.
Parts list |
||
|---|---|---|
| Part ID | Characterization | Details |
| K1755001 | ribB coding sequence | more information |
| K1755002 | ribB + B0014(terminator) | more information |
| K1755003 | B0032(ribosome binding sequence,RBS) + ribB + B0014(terminator) | more information |
| K1755004 | K525998(PT7+RBS) + ribB + B0014(terminator) | more information |
| K1755005 | K608003(strong promoter+medium RBS) + ribB + B0014(terminator) | more information |
| K1755006 | K608006(medium promoter+medium RBS) + ribB + B0014(terminator) | more information |
| K1755007 | B608002(strong promoter+strong RBS)+ ribB + B0014(terminator) | more information |
| K1755008 | K608004(strong Promoter and weak RBS)+ribB+B0014(Double terminator) | more information |
| K1755009 | K608007(Medium Promoter and weak RBS)+ribB+B0014(Double terminator) | more information |
| K1755301 | MarO(Copper sensor) + B0032(RBS) + ribB + B0014(terminator) | more information |
| K1755302 | K190024(Copper sensor) + ribB + B0014(terminator) | more information |
| K1755303 | K540001（cobalt sensor） + B0032(RBS) + ribB + B0014(terminator) | more information |
| K1755305 | K346002(MerO) + B0032(RBS) + ribB + B0014(terminator) | more information |
| K1755024 | K911003+ B0032(RBS) + ribB + B0014(terminator) | more information |