Difference between revisions of "Team:CGU Taiwan/Notebook"
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<tr><td> Name</td><td>Pur. </td><td>Seq.(5’-3’) </td><td>Size (mer.) </td><td> MW (g/mol)</td><td>Tm (℃)</td><td>GC (%)</td><td>Nmole</td><td>μl for 100μM </td></tr> | <tr><td> Name</td><td>Pur. </td><td>Seq.(5’-3’) </td><td>Size (mer.) </td><td> MW (g/mol)</td><td>Tm (℃)</td><td>GC (%)</td><td>Nmole</td><td>μl for 100μM </td></tr> | ||
Revision as of 12:36, 17 September 2015
GPCR
Operator: Wan yun, Jinting , Jinye
Goal:
1. Extraction of gDNA of Far1∆::KANMX strain
2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
3. PCR gDNA of Far1∆ ::KANMX
4. Electrophoresis to check PCR product
Experiment steps:
< Extraction of gDNA of Far1∆::KANMX strain>
Consult the protocol
1. Design of primers
2. PCR program
Goal:
1. Extraction of gDNA of Far1∆::KANMX strain
2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
3. PCR gDNA of Far1∆ ::KANMX
4. Electrophoresis to check PCR product
Experiment steps:
< Extraction of gDNA of Far1∆::KANMX strain>
Consult the protocol
| Strains | 260/280 | 260/230 | C(ng/μl) |
| Far1∆::KANMX strain | 1.62 | 0.97 | 44.7 |
1. Design of primers
| Name | Pur. | Seq.(5’-3’) | Size (mer.) | MW (g/mol) | Tm (℃) | GC (%) | Nmole | μl for 100μM | ||
| dFAR1 F’ | Desalt | ggTTTTgTTAggCgggCAAg | 20 | 6244.1 | 53.8 | 55 | 21.25 | 212.50 | ||
| dFAR1 R’ | Desalt | CATTAACTgCTATTTACgACgC | 22 | 6669.4 | 51.1 | 41 | 17.12 | 171.20 |
| Step | Temperature | Time |
| Step1 | 95℃ | 5min |
| Step2 | 95℃ | 30s |
| Step3 | 52℃ | 30s |
| Step4→step2 for 30 cycle | 72℃ | 2min |
| Step5 | 72℃ | 5min |
| Step6 (hold on) | 10℃ | 1h |
Operator: Wan yun, Jinting , Jinye
Goal:
1. Extraction of gDNA of Far1∆::KANMX strain
2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
3. PCR gDNA of Far1∆ ::KANMX
4. Electrophoresis to check PCR product
Experiment steps:
< Extraction of gDNA of Far1∆::KANMX strain>
Consult the protocol
1. Design of primers
| Strains | 260/280 | 260/230 | C(ng/μl) |
| Far1∆::KANMX strain | 1.62 | 0.97 | 44.7 |
| Tm (℃) | GC (%) | Nmole | μl for 100μM | |||||
| 55 | 21.25 | 212.50 | ||||||
| 41 | 17.12 | 171.20 |
Operator: Wan yun, Jinting , Jinye
Goal:
1. Extraction of gDNA of Far1∆::KANMX strain
2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
3. PCR gDNA of Far1∆ ::KANMX
4. Electrophoresis to check PCR product
Experiment steps:
< Extraction of gDNA of Far1∆::KANMX strain>
Consult the protocol
1. Design of primers
| Strains | 260/280 | 260/230 | C(ng/μl) |
| Far1∆::KANMX strain | 1.62 | 0.97 | 44.7 |
| Tm (℃) | GC (%) | Nmole | μl for 100μM | |||||
| 55 | 21.25 | 212.50 | ||||||
| 41 | 17.12 | 171.20 |
Operator: Wan yun, Jinting , Jinye
Goal:
1. Extraction of gDNA of Far1∆::KANMX strain
2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
3. PCR gDNA of Far1∆ ::KANMX
4. Electrophoresis to check PCR product
Experiment steps:
< Extraction of gDNA of Far1∆::KANMX strain>
Consult the protocol
1. Design of primers
| Strains | 260/280 | 260/230 | C(ng/μl) |
| Far1∆::KANMX strain | 1.62 | 0.97 | 44.7 |
| Tm (℃) | GC (%) | Nmole | μl for 100μM | |||||
| 55 | 21.25 | 212.50 | ||||||
| 41 | 17.12 | 171.20 |
Operator: Wan yun, Jinting , Jinye
Goal:
1. Extraction of gDNA of Far1∆::KANMX strain
2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
3. PCR gDNA of Far1∆ ::KANMX
4. Electrophoresis to check PCR product
Experiment steps:
< Extraction of gDNA of Far1∆::KANMX strain>
Consult the protocol
1. Design of primers
| Strains | 260/280 | 260/230 | C(ng/μl) |
| Far1∆::KANMX strain | 1.62 | 0.97 | 44.7 |
| Tm (℃) | GC (%) | Nmole | μl for 100μM | |||||
| 55 | 21.25 | 212.50 | ||||||
| 41 | 17.12 | 171.20 |
Operator: Wan yun, Jinting , Jinye
Goal:
1. Extraction of gDNA of Far1∆::KANMX strain
2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
3. PCR gDNA of Far1∆ ::KANMX
4. Electrophoresis to check PCR product
Experiment steps:
< Extraction of gDNA of Far1∆::KANMX strain>
Consult the protocol
1. Design of primers
| Strains | 260/280 | 260/230 | C(ng/μl) |
| Far1∆::KANMX strain | 1.62 | 0.97 | 44.7 |
| Tm (℃) | GC (%) | Nmole | μl for 100μM | |||||
| 55 | 21.25 | 212.50 | ||||||
| 41 | 17.12 | 171.20 |
Operator: Wan yun, Jinting , Jinye
Goal:
1. Extraction of gDNA of Far1∆::KANMX strain
2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
3. PCR gDNA of Far1∆ ::KANMX
4. Electrophoresis to check PCR product
Experiment steps:
< Extraction of gDNA of Far1∆::KANMX strain>
Consult the protocol
1. Design of primers
| Strains | 260/280 | 260/230 | C(ng/μl) |
| Far1∆::KANMX strain | 1.62 | 0.97 | 44.7 |
| Tm (℃) | GC (%) | Nmole | μl for 100μM | |||||
| 55 | 21.25 | 212.50 | ||||||
| 41 | 17.12 | 171.20 |
Operator: Wan yun, Jinting , Jinye
Goal:
1. Extraction of gDNA of Far1∆::KANMX strain
2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
3. PCR gDNA of Far1∆ ::KANMX
4. Electrophoresis to check PCR product
Experiment steps:
< Extraction of gDNA of Far1∆::KANMX strain>
Consult the protocol
1. Design of primers
| Strains | 260/280 | 260/230 | C(ng/μl) |
| Far1∆::KANMX strain | 1.62 | 0.97 | 44.7 |
| Tm (℃) | GC (%) | Nmole | μl for 100μM | |||||
| 55 | 21.25 | 212.50 | ||||||
| 41 | 17.12 | 171.20 |
RNA
Operator: Wan yun, Jinting , Jinye
Goal:
1. Extraction of gDNA of Far1∆::KANMX strain
2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
3. PCR gDNA of Far1∆ ::KANMX
4. Electrophoresis to check PCR product
Experiment steps:
< Extraction of gDNA of Far1∆::KANMX strain>
Consult the protocol
1. Design of primers
| Strains | 260/280 | 260/230 | C(ng/μl) |
| Far1∆::KANMX strain | 1.62 | 0.97 | 44.7 |
| Tm (℃) | GC (%) | Nmole | μl for 100μM | |||||
| 55 | 21.25 | 212.50 | ||||||
| 41 | 17.12 | 171.20 |