Difference between revisions of "Template:SJTU-BioX-Shanghai/Notebook/Transformation/w10"

(→‎pcpcG2 electro-transformation)
(→‎first desalination test of pcpcG2-HR)
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5th liquid segregation of pcpcG2-HR
 
5th liquid segregation of pcpcG2-HR
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===first desalination test of pcpcG2-HR===
 
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=====Sep. 7=====
 
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Inoculated 15 wildtype & 15 pcpcG2-HR (use pcpcG2-HR from colony 2) at 10pm.
 
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=====Sep. 9=====
 
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Induced with green light at 10pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each).
 
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=====Sep. 10=====
 
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Cultivated with red light at 10am, then took the second 6 samples,
 
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Took the third 6 samples at 2pm.
 
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Took the fourth 6 samples at 10pm.
 
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=====Sep. 11=====
 
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Took the fifth 6 samples at 10am.
 
  
 
===pdark-GFP electro-transformation===
 
===pdark-GFP electro-transformation===

Revision as of 13:35, 17 September 2015

SJTUB transformation logo.jpg Transformation

pdark natural transformation

In the evening of Sep. 11

6th liquid segregation of pdark-HR

pcpcG2 electro-transformation

In the evening of Sep. 11

5th liquid segregation of pcpcG2-HR

pdark-GFP electro-transformation

Sep. 8

We picked 1 colony (only 1 colony appeared) from the pdark-GFP plate and spread them on another BG11 plate in line to do solid segregation