Difference between revisions of "Team:Aalto-Helsinki/InterLabBook"
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| − | <ul id="sidenav" class="nav nav-stacked" | + | <ul id="sidenav" class="nav nav-stacked"> |
| − | <li><a href="#week1"><h4>Week 1</h4></a></li> | + | <li><a href="#" data-scroll="week1"><h4>Week 1</h4></a></li> |
| − | <li><a href="#week2"><h4>Week 2</h4></a></li> | + | <li><a href="#" data-scroll="week2"><h4>Week 2</h4></a></li> |
| − | <li><a href="# | + | <li><a href="#" data-scroll="week3"><h4>Weeks 3-5</h4></a></li> |
| − | + | <li><a href="#" data-scroll="week6"><h4>Week 6</h4></a></li> | |
| − | <li><a href="# | + | <li><a href="#" data-scroll="week7"><h4>Week 7</h4></a></li> |
| − | + | <li><a href="#" data-scroll="week8"><h4>Weeks 8-10</h4></a></li> | |
| − | <li><a href="# | + | <li><a href="#" ><h4 style="border-top:solid;">To the top</h4></a></li> |
| − | + | <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/InterLab" ><h4>To the Previous Page</h4></a></li> | |
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| + | <div class="plasmids" style="margin-bottom:0;"> | ||
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| − | < | + | <div class = "inner-container" > |
| + | <h1>Interlab Measurement Study Lab Book</h1> | ||
| + | <p>Before reading our Interlab lab book, take a look into this <a href="https://static.igem.org/mediawiki/2015/d/d8/Aalto-Helsinki_interlab_spreadsheet.xls">excel file</a> to find the nomenclature we used for our devices.</p> | ||
</div><!-- end inner-container--> | </div><!-- end inner-container--> | ||
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| − | < | + | <!-- Week 1 below --> |
| + | <section id="week1" class="active" data-anchor="week1" > | ||
| + | <h2>Week 1</h2> | ||
<table > | <table > | ||
<tr> | <tr> | ||
| − | <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;"> | + | <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Worked With</h3></td> |
| − | <td class="bottomline"><h3> | + | <td class="bottomline"><h3>WorkFlow</h3></td> |
<td class="bottomline"></td> | <td class="bottomline"></td> | ||
<td class="bottomline"></td> | <td class="bottomline"></td> | ||
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</td> | </td> | ||
<td class="blue arrow" > | <td class="blue arrow" > | ||
| − | <p> | + | <p>MiniPrep</p> |
</td> | </td> | ||
</tr> | </tr> | ||
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</td> | </td> | ||
</tr> | </tr> | ||
| − | |||
</table> | </table> | ||
| − | < | + | <div class="inner-container" style="margin: 5% 20% 5% 25%;"> |
| + | <p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/8/87/Aalto-Helsinki_interlab1.pdf" target="_blank">here</a> to read the full lab notes of week 1.</p></div> | ||
| + | |||
| + | </section> | ||
| + | <!-- Week 1 above --> | ||
| + | |||
| + | |||
| + | <!-- Week 2 below --> | ||
| + | <section id="week2" data-anchor="week2"> | ||
| + | <h2>Week 2</h2> | ||
<table > | <table > | ||
<tr> | <tr> | ||
| − | <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;"> | + | <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Worked With</h3></td> |
| − | <td class="bottomline"><h3> | + | <td class="bottomline"><h3>WorkFlow</h3></td> |
<td class="bottomline"></td> | <td class="bottomline"></td> | ||
<td class="bottomline"></td> | <td class="bottomline"></td> | ||
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<td class="bottomline"></td> | <td class="bottomline"></td> | ||
<td class="bottomline"></td> | <td class="bottomline"></td> | ||
| − | <td class="bottomline"><h3>June 29th - <br/> July | + | <td class="bottomline"><h3>June 29th - <br/> July 3rd</h3></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="first rightline"><div class="heightdiv"><h3>Preparations</h3></div></td> | <td class="first rightline"><div class="heightdiv"><h3>Preparations</h3></div></td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
| − | <p>Transform <br/> AHD6 & AHD7 <br/>into TOP10</p> | + | <p style="font-size:12px">Transform <br/> AHD6 & AHD7 <br/>into TOP10</p> |
</td> | </td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
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</td> | </td> | ||
<td class="blue arrow" > | <td class="blue arrow" > | ||
| − | <p> | + | <p>MiniPrep</p> |
</td> | </td> | ||
</tr> | </tr> | ||
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</table> | </table> | ||
| − | < | + | <div class="inner-container" style="margin: 5% 20% 5% 25%;"> |
| + | <p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/3/3f/Aalto-Helsinki_interlab2.pdf" target="_blank">here</a> to read the full lab notes of week 2.</p></div> | ||
| + | |||
| + | </section> | ||
| + | <!-- Week 2 above --> | ||
| + | |||
| + | |||
| + | <!-- Weeks 3-5 below --> | ||
| + | <section id="week3" data-anchor="week3"> | ||
| + | <h2>Weeks 3-5</h2> | ||
<table> | <table> | ||
<tr> | <tr> | ||
| − | <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;"> | + | <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Worked With</h3></td> |
| − | <td class="bottomline"><h3> | + | <td class="bottomline"><h3>WorkFlow</h3></td> |
<td class="bottomline"></td> | <td class="bottomline"></td> | ||
<td class="bottomline"></td> | <td class="bottomline"></td> | ||
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<td class="bottomline"></td> | <td class="bottomline"></td> | ||
<td class="bottomline"></td> | <td class="bottomline"></td> | ||
| − | <td class="bottomline"><h3>July 6th - <br/> July | + | <td class="bottomline"><h3>July 6th - <br/> July 24th</h3></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="first rightline"><div class="heightdiv"><h3>Checking D2</h3></div></td> | <td class="first rightline"><div class="heightdiv"><h3>Checking D2</h3></div></td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
| − | <p>Transform | + | <p>Transform D2 <br/>into TOP10</p> |
</td> | </td> | ||
<td class="blue arrow" > | <td class="blue arrow" > | ||
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</td> | </td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
| − | <p>Stored in -20°C</p> | + | <p>Stored <br/>in -20°C</p> |
</td> | </td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
| + | |||
| + | <div class="inner-container" style="margin: 5% 20% 5% 25%;"> | ||
| + | <p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/f/f7/Aalto-Helsinki_interlab3-4-correct.pdf" target="_blank">here</a> to read the full lab notes of weeks 3-5.</p></div> | ||
| + | |||
| + | </section> | ||
| + | <!-- Weeks 3-5 above --> | ||
| − | < | + | <!-- Week 6 below --> |
| + | <section id="week6" data-anchor="week6"> | ||
| + | <h2>Week 6</h2> | ||
<table> | <table> | ||
<tr> | <tr> | ||
| − | <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;"> | + | <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Worked With</h3></td> |
| − | <td class="bottomline"><h3> | + | <td class="bottomline"><h3>WorkFlow</h3></td> |
<td class="bottomline"></td> | <td class="bottomline"></td> | ||
<td class="bottomline"></td> | <td class="bottomline"></td> | ||
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<td class="first rightline"><div class="heightdiv"><h3>Checking</h3></div></td> | <td class="first rightline"><div class="heightdiv"><h3>Checking</h3></div></td> | ||
<td class="white arrow"> | <td class="white arrow"> | ||
| − | <p>Check AHD1 <br/>& AHD3-AHD5 <br/> restrictions on gel</p> | + | <p style="font-size:12px">Check AHD1 <br/>& AHD3-AHD5 <br/> restrictions on gel</p> |
</td> | </td> | ||
<td class="blue arrow" > | <td class="blue arrow" > | ||
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</td> | </td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
| − | <p>Too little <br/>restriction mix<br/> for new ligation</p> | + | <p style="font-size:12px">Too little <br/>restriction mix<br/> for new ligation</p> |
</td> | </td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
| Line 405: | Line 318: | ||
</td> | </td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
| − | <p>Stored in -20°C</p> | + | <p>Stored <br/>in -20°C</p> |
</td> | </td> | ||
</tr> | </tr> | ||
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</table> | </table> | ||
| − | < | + | <div class="inner-container" style="margin: 5% 20% 5% 25%;"> |
| + | <p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/d/dd/Aalto-Helsinki_interlab6-correct.pdf" target="_blank">here</a> to read the full lab notes of week 6.</p></div> | ||
| + | |||
| + | |||
| + | |||
| + | </section> | ||
| + | <!-- Week 6 above --> | ||
| + | |||
| + | |||
| + | <!-- Week 7 below --> | ||
| + | <section id="week7" data-anchor="week7"> | ||
| + | <h2>Week 7</h2> | ||
<table> | <table> | ||
<tr> | <tr> | ||
| − | <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;"> | + | <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Worked With</h3></td> |
| − | <td class="bottomline"><h3> | + | <td class="bottomline"><h3>WorkFlow</h3></td> |
<td class="bottomline"></td> | <td class="bottomline"></td> | ||
<td class="bottomline"></td> | <td class="bottomline"></td> | ||
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<p>No GFP <br/> expression <br/> in D3</p> | <p>No GFP <br/> expression <br/> in D3</p> | ||
</td> | </td> | ||
| − | <td class=" | + | <td class="white arrow" > |
<p>Re-restrict <br/>AHD3-AHD5</p> | <p>Re-restrict <br/>AHD3-AHD5</p> | ||
</td> | </td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
| − | <p>Ligate & < | + | <p>Ligate & <br/> transform <br/> into TOP10</p> |
</td> | </td> | ||
<td class="red arrow" > | <td class="red arrow" > | ||
| − | <p>No GFP expression</p> | + | <p>No GFP <br/>expression</p> |
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td class="first rightline"><div class="heightdiv"><h3>Checking</h3></div></td> | ||
| + | |||
| + | <td class="white arrow" > | ||
| + | <p style="font-size:12px">Mistake in AHD4 <br/>restriction <br/>protocol</p> | ||
| + | </td> | ||
| + | <td class="white arrow" > | ||
| + | <p>Check AHD4 <br/>restriction <br/>on gel</p> | ||
| + | </td> | ||
| + | <td class="blue arrow" > | ||
| + | <p>Restriction OK</p> | ||
</td> | </td> | ||
</tr> | </tr> | ||
| − | |||
</table> | </table> | ||
| − | < | + | <div class="inner-container" style="margin: 5% 20% 5% 25%;"> |
| − | + | <p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/0/07/Aalto-Helsinki_interlab7.pdf" target="_blank">here</a> to read the full lab notes of week 7.</p></div> | |
| − | < | + | |
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| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
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| − | + | ||
| − | + | ||
| + | </section> | ||
| + | <!-- Week 7 above --!> | ||
| + | |||
| + | |||
| + | <!-- Weeks 8-9 below --> | ||
| + | <section id="week8" data-anchor="week8"> | ||
| + | <h2>Weeks 8-10</h2> | ||
| + | |||
| + | |||
| + | <table> | ||
| + | <tr> | ||
| + | <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Worked With</h3></td> | ||
| + | <td class="bottomline"><h3>WorkFlow</h3></td> | ||
| + | <td class="bottomline"></td> | ||
| + | <td class="bottomline"></td> | ||
| + | <td class="bottomline"></td> | ||
| + | <td class="bottomline"></td> | ||
| + | <td class="bottomline"></td> | ||
| + | <td class="bottomline"><h3>August 10th - <br/> August 28th</h3></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td class="first rightline"><div class="heightdiv"><h3>Measurement</h3></div></td> | ||
| + | <td class="white arrow"> | ||
| + | <p>Transform <br/> D1 & D2 <br/> into TOP10</p> | ||
| + | </td> | ||
| + | <td class="white arrow"> | ||
| + | <p>o/n cultures</p> | ||
| + | </td> | ||
| + | <td class="white arrow"> | ||
| + | <p>Measure GFP</p> | ||
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td class="first rightline"><div class="heightdiv"><h3>Checking</h3></div></td> | ||
| + | <td class="blue arrow"> | ||
| + | <p>Restrict & check <br/>D1 & D2 <br/>on gel</p> | ||
| + | </td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | |||
| + | <div class="inner-container" style="margin: 5% 20% 5% 25%;"> | ||
| + | <p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/7/70/Aalto-Helsinki_weeks8-9.pdf" target="_blank">here</a> to read the full lab notes of weeks 8-10.</p></div> | ||
| + | |||
| + | |||
| + | <!-- Weeks 8-10 above --!> | ||
| + | <div class="inner-container" style="margin:5% 20% 0% 25%;padding-bottom:10%;"> | ||
| + | <p style="font-size:16px">Due to the limited time we had to use for our project, we were only able to construct two devices and measure the GFP from these. We were unable to build the device with J23106 promoter.</p> | ||
| + | |||
| + | <p style="font-size:16px">Our results can be found on our <a href="https://2015.igem.org/Team:Aalto-Helsinki/InterLab">Interlab Study main page</a>.</p></div> | ||
| + | |||
| + | </section> | ||
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Latest revision as of 13:35, 17 September 2015
