Difference between revisions of "Template:SJTU-BioX-Shanghai/Notebook/Transformation/w10"

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-{{SJTU-BioX-Shanghai/Notebook/Transformation}}
+===[[file: SJTUB_transformation_logo.jpg | 25px]] Transformation===
-===pdark natural transformation===
-'''in the evening of 11th, Sep'''
-th liquid segregation of pdark-HR
+====pdark natural transformation====
-===pcpcG2 electro-transformation===
+=====In the evening of Sep. 11=====
-'''in the evening of 11th Sep'''
-th liquid segregation of pcpcG2-HR
+th liquid segregation of pdark-HR
-===first desalination test of pcpcG2-HR===
+====pcpcG2 electro-transformation====
-'''on 7th, Sep'''
-Inoculated 15 wildtype & 15 pcpcG2-HR (use pcpcG2-HR from colony 2) at 10pm
+=====In the evening of Sep. 11=====
-'''on 9th, Sep'''
+th liquid segregation of pcpcG2-HR
-Induced with green light at 10pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each)
-'''on 10th, Sep'''
-cultivated with red light at 10am, then took the second 6 samples,
-took the third 6 samples at 2pm
-took the fourth 6 samples at 10pm
-'''on 11th, Sep'''
-took the fifth 6 samples at 10am
+====pdark-GFP electro-transformation====
-===pdark-GFP electro-transformation===
+=====Sep. 8=====
-'''on 8th, Sep'''
 We picked 1 colony (only 1 colony appeared) from the pdark-GFP plate and spread them on another BG11 plate in line to do solid segregation
-<html></div></html>

Latest revision as of 13:35, 17 September 2015