Difference between revisions of "Template:SJTU-BioX-Shanghai/Notebook/Transformation/w10"

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 ===[[file: SJTUB_transformation_logo.jpg | 25px]] Transformation===
-===pdark natural transformation===
+====pdark natural transformation====
-'''in the evening of 11th, Sep'''
+=====In the evening of Sep. 11=====
 th liquid segregation of pdark-HR
-===pcpcG2 electro-transformation===
+====pcpcG2 electro-transformation====
-'''in the evening of 11th Sep'''
+=====In the evening of Sep. 11=====
 th liquid segregation of pcpcG2-HR
-===first desalination test of pcpcG2-HR===
+====pdark-GFP electro-transformation====
-=====Sep. 7=====
-Inoculated 15 wildtype & 15 pcpcG2-HR (use pcpcG2-HR from colony 2) at 10pm.
-=====Sep. 9=====
-Induced with green light at 10pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each).
-=====Sep. 10=====
-Cultivated with red light at 10am, then took the second 6 samples,
-Took the third 6 samples at 2pm.
-Took the fourth 6 samples at 10pm.
-=====Sep. 11=====
-Took the fifth 6 samples at 10am.
-===pdark-GFP electro-transformation===
+=====Sep. 8=====
-'''on 8th, Sep'''
 We picked 1 colony (only 1 colony appeared) from the pdark-GFP plate and spread them on another BG11 plate in line to do solid segregation

Latest revision as of 13:35, 17 September 2015