Difference between revisions of "Template:SJTU-BioX-Shanghai/Notebook/Transformation/w10"

(pcpcG2 electro-transformation)
(pdark-GFP electro-transformation)
 
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===[[file: SJTUB_transformation_logo.jpg | 25px]] Transformation===
 
===[[file: SJTUB_transformation_logo.jpg | 25px]] Transformation===
  
===pdark natural transformation===
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====pdark natural transformation====
  
=====in the evening of Sep. 11=====
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=====In the evening of Sep. 11=====
  
 
6th liquid segregation of pdark-HR
 
6th liquid segregation of pdark-HR
  
===pcpcG2 electro-transformation===
+
====pcpcG2 electro-transformation====
  
 
=====In the evening of Sep. 11=====
 
=====In the evening of Sep. 11=====
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5th liquid segregation of pcpcG2-HR
 
5th liquid segregation of pcpcG2-HR
  
===first desalination test of pcpcG2-HR===
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====pdark-GFP electro-transformation====
 
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=====Sep. 7=====
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Inoculated 15 wildtype & 15 pcpcG2-HR (use pcpcG2-HR from colony 2) at 10pm.
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=====Sep. 9=====
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Induced with green light at 10pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each).
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=====Sep. 10=====
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Cultivated with red light at 10am, then took the second 6 samples,
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Took the third 6 samples at 2pm.
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Took the fourth 6 samples at 10pm.
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=====Sep. 11=====
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Took the fifth 6 samples at 10am.
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===pdark-GFP electro-transformation===
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=====Sep. 8=====
 
=====Sep. 8=====
  
 
We picked 1 colony (only 1 colony appeared) from the pdark-GFP plate and spread them on another BG11 plate in line to do solid segregation
 
We picked 1 colony (only 1 colony appeared) from the pdark-GFP plate and spread them on another BG11 plate in line to do solid segregation

Latest revision as of 13:35, 17 September 2015

SJTUB transformation logo.jpg Transformation

pdark natural transformation

In the evening of Sep. 11

6th liquid segregation of pdark-HR

pcpcG2 electro-transformation

In the evening of Sep. 11

5th liquid segregation of pcpcG2-HR

pdark-GFP electro-transformation

Sep. 8

We picked 1 colony (only 1 colony appeared) from the pdark-GFP plate and spread them on another BG11 plate in line to do solid segregation