Difference between revisions of "Template:SJTU-BioX-Shanghai/Notebook/Transformation/w8"

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 ====pdark natural transformation====
-'''in the evening of 29th, Aug'''
+=====In the evening of Aug. 29=====
 nd liquid segregation of pdark-HR
 ====pcpcG2 electro-transformation====
-'''in the evening of 24th, Aug'''
+=====In the evening of Aug. 24=====
 We picked 4 colonies (marked with 1,2,3 & 4) from the pcpcG2-HR plate and spread them on another BG11 plate in parallel seprately to do solid segregation
-'''in the evening of 30th, Aug'''
+=====In the evening of Aug. 30=====
 We scraped the grown cell from the plate, which is 2,3 & 4 to do colony PCR, 1st liquid segregation and solid storage
 ====pre-experiment of pdark-HR====
-'''on 24th, Aug'''
+=====Aug. 24=====
 Took the third 4 samples at 9am & fourth 4 samples at 9pm.
-'''on 25th, Aug'''
+=====Aug. 25=====
 Took the fifth 4 samples at 9am & sixth 4 samples at 9pm.
-'''on 26th, Aug'''
+=====Aug. 26=====
 Took the seventh 4 samples at 9am & eighth 4 samples at 9pm.
 ====pdark-GFP electro-transformation====
-'''in the evening of 24th, Aug'''
+=====In the evening of Aug. 24=====
 Set culture.
-'''in the evening of 26th, Aug'''
+=====In the evening of Aug. 26=====
 Electro-transformation of pdark-GFP.
-'''in the evening of 27th, Aug'''
+=====In the evening of Aug. 27=====
 Collected half of the cell and mixed with 5ml soft agar, then poured on solid medium plate.

Latest revision as of 13:40, 17 September 2015