Difference between revisions of "Team:NRP-UEA-Norwich/Parts"
| Line 119: | Line 119: | ||
</tr> | </tr> | ||
| − | <tr><td>GlgX</td><td>pSB1C3</td><td>Debranching | + | <tr><td>GlgX</td><td>pSB1C3</td><td>Debranching Enzyme <i>(E. coli)</i></td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618025" style = "color: #002bb8;">BBa_K1618025</a></td></tr> |
<tr><td>GlgB</td><td>pSB1C3</td><td>Branching Enzyme <i>(E. coli)</i></td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618000" style = "color: #002bb8;">BBa_K1618000</a></td></tr> | <tr><td>GlgB</td><td>pSB1C3</td><td>Branching Enzyme <i>(E. coli)</i></td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618000" style = "color: #002bb8;">BBa_K1618000</a></td></tr> | ||
| Line 147: | Line 147: | ||
<th>Name in registry</th> | <th>Name in registry</th> | ||
</tr> | </tr> | ||
| − | <tr><td>P-Lac(R0010)_RBS(B0034)_GlgX(K1618025)</td><td>pSB1C3</td><td>Debranching | + | <tr><td>P-Lac(R0010)_RBS(B0034)_GlgX(K1618025)</td><td>pSB1C3</td><td>Debranching Enzyme Expression Construct</td><td>Removes Glycogen Branches</td><td><a href="http://parts.igem.org/Part:BBa_K1618026" style = "color: #002bb8;">BBa_K1618026</a></td></tr> |
| − | <tr><td>P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)</td><td>pSB1C3</td><td>Branching | + | <tr><td>P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)</td><td>pSB1C3</td><td>Branching Enzyme Expression Construct</td><td>Adds Glycogen Branches</td><td><a href="http://parts.igem.org/Part:BBa_K1618022" style = "color: #002bb8;">BBa_K1618022</a></td></tr> |
| − | <tr><td>P-Lac(R0010)_RBS(B0034)_MAO(K1618001)</td><td>pSB1C3</td><td> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_MAO(K1618001)</td><td>pSB1C3</td><td>Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td><td><a href="http://parts.igem.org/Part:BBa_K1618005" style = "color: #002bb8;">BBa_K1618005</a> </td></tr> |
| − | <tr><td>P-Lac(R0010)_RBS(B0034)_Rv3030(K1618002)</td><td>pSB1C3</td><td> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_Rv3030(K1618002)</td><td>pSB1C3</td><td>Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td><td><a href="http://parts.igem.org/Part:BBa_K1618007" style = "color: #002bb8;">BBa_K1618007</a></td></tr> |
| − | <tr><td>P-Lac(R0010)_RBS(B0034)_Rv3034c(K1618003)</td><td>pSB1C3</td><td> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_Rv3034c(K1618003)</td><td>pSB1C3</td><td>Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td> <td><a href="http://parts.igem.org/Part:BBa_K1618008" style = "color: #002bb8;">BBa_K1618008</a></td></tr> |
| − | <tr><td>P-Lac(R0010)_RBS(B0034)_Rv3037c(K1618004)</td><td>pSB1C3</td><td> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_Rv3037c(K1618004)</td><td>pSB1C3</td><td>Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td> <td><a href="http://parts.igem.org/Part:BBa_K1618009" style = "color: #002bb8;">BBa_K1618009</a></td></tr> |
| − | <tr><td>P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3030(K1618002)</td><td>pSB1C3</td><td>Branching | + | <tr><td>P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3030(K1618002)</td><td>pSB1C3</td><td>Branching Enzyme + Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td><td><a href="http://parts.igem.org/Part:BBa_K1618023" style = "color: #002bb8;">BBa_K1618023</a></td></tr> |
| − | <tr><td>P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3034c(K1618003)</td><td>pSB1C3</td><td>Branching | + | <tr><td>P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3034c(K1618003)</td><td>pSB1C3</td><td>Branching Enzyme + Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td><td><a href="http://parts.igem.org/Part:BBa_K1618024" style = "color: #002bb8;">BBa_K1618024</a></td></tr> |
| − | <tr><td>P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3037c(K1618004)</td><td>pSB1C3</td><td> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3037c(K1618004)</td><td>pSB1C3</td><td>Branching Enzyme + Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td><td><a href="http://parts.igem.org/Part:BBa_K1618027" style = "color: #002bb8;">BBa_K1618027</a></td></tr> |
</table> | </table> | ||
Revision as of 14:01, 17 September 2015
Parts
BASIC PARTS FOR ENGINEERING BACTERIAL GLYCOGEN
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| GlgX | pSB1C3 | Debranching Enzyme (E. coli) | Coding sequence only | BBa_K1618025 |
| GlgB | pSB1C3 | Branching Enzyme (E. coli) | Coding sequence only | BBa_K1618000 |
| MAO | pSB1C3 | Acyltransferase Candidate 1 | Coding sequence only | BBa_K1618001 |
| Rv3030 | pSB1C3 | Acyltransferase Candidate 2 | Coding sequence only | BBa_K1618002 |
| Rv3034c | pSB1C3 | Acyltransferase Candidate 3 | Coding sequence only | BBa_K1618003 |
| Rv3037c | pSB1C3 | Acyltransferase Candidate 4 | Coding sequence only | BBa_K1618004 |
COMPOUND PARTS FOR ENGINEERING BACTERIAL GLYCOGEN
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| P-Lac(R0010)_RBS(B0034)_GlgX(K1618025) | pSB1C3 | Debranching Enzyme Expression Construct | Removes Glycogen Branches | BBa_K1618026 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000) | pSB1C3 | Branching Enzyme Expression Construct | Adds Glycogen Branches | BBa_K1618022 |
| P-Lac(R0010)_RBS(B0034)_MAO(K1618001) | pSB1C3 | Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618005 |
| P-Lac(R0010)_RBS(B0034)_Rv3030(K1618002) | pSB1C3 | Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618007 |
| P-Lac(R0010)_RBS(B0034)_Rv3034c(K1618003) | pSB1C3 | Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618008 |
| P-Lac(R0010)_RBS(B0034)_Rv3037c(K1618004) | pSB1C3 | Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618009 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3030(K1618002) | pSB1C3 | Branching Enzyme + Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618023 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3034c(K1618003) | pSB1C3 | Branching Enzyme + Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618024 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3037c(K1618004) | pSB1C3 | Branching Enzyme + Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618027 |
COMPOUND PARTS FROM THE BUTYRATE BIOSYNTHESIS PATHWAY (Coding sequences from Coprococcus sp. L2-50 -DSM 16842-)
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| P-Lac(R0010)_RBS(B0034)_Buk | pSB1C3 | Butyrate kinase expression contruct | Catalyzes the conversionbutyrate to/from butyryl | BBa_K1618012 |
| P-Lac(R0010)_RBS(B0034)_PTB | pSB1C3 | Phosphotransbutrylase expression construct | Catalyzes the conversion butyryl to/from butyryl CoA | BBa_K1618013 |
| P-Lac(R0010)_RBS(B0034)_Buk_PTB | pSB1C3 | Butyrate to butyryl CoA pathway | Catalyzes the conversion butyryl to/from butyryl CoA | BBa_K1618020 |
| P-Lac(R0010)_RBS(B0034)_THL | pSB1C3 | Thiolase expression construct | Catalyzes the conversion of Acetyl-CoA to Acetoacetyl-CoA | BBa_K1618014 |
| P-Lac(R0010)_RBS(B0034)_CRO | pSB1C3 | Crotonase expression construct | Catalyzes conversion of3-hydroxybutyryl-CoA to Crotonyl-CoA | BBa_K1618015 |
| P-Lac(R0010)_RBS(B0034)_BHBD | pSB1C3 | β-hydroxybutyryl-CoA dehydrogenase expression | Catalyzes conversion of Acetoacetyl-CoA to 3-hydroxybutyryl-CoA | BBa_K1618016 |
| P-Lac(R0010)_RBS(B0034)_BCD | pSB1C3 | Butyryl-CoA dehydrogenase expression construct | Catalyzes conversion of Crotonyl-CoA to Butyryl-CoA | BBa_K1618017 |
| P-Lac(R0010)_RBS(B0034)_EFTb | pSB1C3 | Electron transfer protein β expression construct | Catalyzes conversion of acetate to butyrate | BBa_K1618018 |
| P-Lac(R0010)_RBS(B0034)_EFTa | pSB1C3 | Electron transfer protein α expression construct | Catalyzes conversion of acetate to butyrate | BBa_K1618019 |
| P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD-BCD-EFTb-EFTa | pSB1C3 | Acetyl-coA to Butryl-CoA pathway | Catalyzes conversion of Acetyl-coA to Butryl-CoA | BBa_K1618021 |
| P-Lac(R0010)_RBS(B0034)_Buk-PTB-THL-CRO-BHBD-BCD-EFTb-EFTa | pSB1C3 | Acetyl coA to butyrate complete biosynthetic pathway | Catalyzes conversion of Acetyl coA to butyrate | BBa_K1618046 |
COMPOUND PARTS FOR ENGINEERING PLANT STARCH
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| P-35s(K1467101)_TP(K1618037):MAO(K1618001)_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 1 expression construct | Transfers acyl groups to starch | BBa_K1618033 |
| P-35s(K1467101)_TP(K1618037):Rv3030(K1618002)_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 2 expression construct | Transfers acyl groups to starch | BBa_K1618034 |
| P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003)_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 3 expression construct | Transfers acyl groups to starch | BBa_K1618035 |
| P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004)_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 4 expression construct | Transfers acyl groups to starch | BBa_K1618036 |
| P-35s(K1467101)_TP(K1618037):MAO(K1618001):GFP(K1467204)_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 1 -fused to GFP -expression construct | Check localization in plastid of candidate gene | BBa_K1618029 |
| P-35s(K1467101)_TP(K1618037):Rv3030(K1618002):GFP(K1467204_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 2 -fused to GFP -expression construct | Check localization in plastid of candidate gene | BBa_K1618030 |
| P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003):GFP(K1467204_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 3 -fused to GFP -expression construct | Check localization in plastid of candidate gene | BBa_K1618031 |
| P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004):GFP(K1467204_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 4 -fused to GFP -expression construct | Check localization in plastid of candidate gene | BBa_K1618032 |
| 35s Terminator | pSB1C3** | Eukaryotic terminator from the 35s gene (Cauliflower Mosaic Virus) | terminates transcription in plant cells | BBa_K1618037 |
| Chloroplast Transit Peptide | pSB1C3*** | Synthetic plastid transit peptide form the consensus of multiple RuBisCo Small Subunit genes | In an N-terminal fusion, this peptide directs proteins to the plant plastid. | BBa_K1618028 |
* This was convered to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467400 (complete transcritional unit flipper) made by the NRP UEA 2014 team. These parts were tested by cloning into a binary plasmid vector backbone prior to delivery to plants leaves using Agrobacterium-tumefaciens mediates trasnfection.
** This was convered to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467300 (terminator flipper) made by the NRP UEA 2014 team.
*** This is assembled in a Golden Gate Universal Acceptor Plasmid (pUAP1) in the pSB1C3 backbone according to RFC 106. It is a Golden Gate part in the plant standard syntax, not a BioBrick.
