Difference between revisions of "Team:CityU HK/Safety"

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<h2>Safety in iGEM</h2>
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<title>Description - iGEM2015 wiki</title>
  
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h4>Safe Project Design</h4>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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        <div class="content-wrap"><div id='wsite-content' class='wsite-elements wsite-not-footer'>
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<h2 class="wsite-content-title" style="text-align:center;"><strong style=""><span "font-size:14.0pt;font-family:&quot;arial&quot;,sans-serif;mso-fareast-font-family:="" &quot;times="" roman&quot;;color:black;mso-font-kerning:0pt;mso-ansi-language:en-us;="" mso-fareast-language:zh-tw;mso-bidi-language:ar-sa"="" style=""><font color="#4caac9">The organisms used for this project:</font></span></strong><br /></h2>
  
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h4>Safe Lab Work</h4>
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    <th class="tg-hgcj">Species</th>
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    <td class="tg-s6z2">K-12</td>
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    <td class="tg-s6z2">1</td>
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    <td class="tg-s6z2" rowspan="2">E. coli K-12 is considered as having no or <br>low individual and community risk. It is <br>unlikely to cause human or animal disease.</td>
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    <td class="tg-s6z2">K-12</td>
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    <td class="tg-s6z2">JM109</td>
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    <td class="tg-s6z2">1</td>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
  
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<h4>Safe Shipment</h4>
 
  
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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<div class="paragraph" style="text-align:left;"><ul><li><font color="#2a2a2a"><span style="font-size: medium; line-height: 1.5em; background-color: initial;">All E. coli strains used in this project are attenuated mutant strains that</span><span style="font-size: medium; line-height: 1.5em; background-color: initial;"> </span><span style="font-size: medium; line-height: 1.5em; background-color: initial;">are unlikely to survive/propagate in the environment outside the</span><span style="font-size: medium; line-height: 1.5em; background-color: initial;"> </span><span style="background-color: initial;"><font size="3"><span style="line-height: 1.5em;">laboratory as they&nbsp;</span><span style="line-height: 24px;">harbor</span><span style="line-height: 1.5em;">&nbsp;many genetic&nbsp;mutations in their genome.&nbsp;</span></font></span></font></li><li><font color="#2a2a2a"><span style="font-size: medium; line-height: 1.5em; background-color: initial;">To reduce the risk of biological materials escaping from the lab, all</span><span style="font-size: medium; line-height: 1.5em; background-color: initial;"> </span><span style="font-size: medium; line-height: 1.5em; background-color: initial;">bacterial strains are kept and stored in designated refrigerators (before and after all experiments) in the supervisor&rsquo;s (Dr. Richard Kong)</span><span style="font-size: medium; line-height: 1.5em; background-color: initial;"> </span></font><span style="font-size: medium; line-height: 1.5em; background-color: initial;"><font color="#2a2a2a">laboratory which is not accessible to the general public. The lab where iGEM experiments are conducted is also not accessible to the general public because all lab workers need to be authorized to work in the lab and are required to carry a &ldquo;name badge&rdquo;.</font></span><br /></li></ul><br /><span style=""></span></div>
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<div class="paragraph" style="text-align:justify;"><ul><li><font color="#2a2a2a"><span "mso-bidi-font-size:12.0pt;font-family:&quot;arial&quot;,sans-serif;="" color:black;background:#f0eeea"="" style="font-size: medium; line-height: 1.5em; background-color: initial;"><strong>Possible risks&nbsp;:&nbsp;</strong>Spillage of bacterial cultures or organic</span><span "mso-bidi-font-size:12.0pt;font-family:="" &quot;arial&quot;,sans-serif;color:black;background:#f0eeea;mso-fareast-language:zh-hk"="" style="font-size: medium; line-height: 1.5em; background-color: initial;"> </span><span "mso-bidi-font-size:12.0pt;font-family:&quot;arial&quot;,sans-serif;="" color:black;background:#f0eeea"="" style="font-size: medium; line-height: 1.5em; background-color: initial;">wastes/solutions&nbsp;</span><br /></font></li><li><font color="#2a2a2a"><span style="font-size: medium; line-height: 1.5em; background-color: initial;"><strong>Solution:</strong> Spillages will be immediately dealt with in the lab using appropriate disinfection procedures (e.g. disinfection with antibacterial detergents) and contaminated bench surface will be cleaned with 70% ethanol.</span><br /></font></li><li><font color="#2a2a2a"><span style="font-size: medium; line-height: 1.5em; background-color: initial;">Organic solvents, biological materials and wastes (microorganisms) and plastic-wares are collected in separate waste containers for</span><span "mso-bidi-font-size:12.0pt;="" font-family:&quot;arial&quot;,sans-serif;color:black;background:#f0eeea;mso-fareast-language:="" zh-hk"="" style="font-size: medium; line-height: 1.5em; background-color: initial;"> </span><span "mso-bidi-font-size:12.0pt;font-family:="" &quot;arial&quot;,sans-serif;color:black;background:#f0eeea"="" style="font-size: medium; line-height: 1.5em; background-color: initial;">collection by technical staff. Biological wastes are treated with bleach</span><span "mso-bidi-font-size:12.0pt;font-family:&quot;arial&quot;,sans-serif;color:black;="" background:#f0eeea;mso-fareast-language:zh-hk"="" style="font-size: medium; line-height: 1.5em; background-color: initial;"> </span></font><span "mso-bidi-font-size:12.0pt;font-family:&quot;arial&quot;,sans-serif;color:black;="" background:#f0eeea"="" style="font-size: medium; line-height: 1.5em; background-color: initial;"><font color="#2a2a2a">and subsequently autoclaved to kill off all remaining microorganisms.</font></span><br /></li></ul><span style=""></span><br /><span style=""></span></div>
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<div class="paragraph" style="text-align:left;"><font size="3"><font color="#2a2a2a"><span "mso-bidi-font-size:12.0pt;font-family:&quot;arial&quot;,sans-serif;="" color:black;mso-themecolor:text1;background:#f0eeea"="" style="">One possible risk is that consumers may suffer from side-effects or complication from ingesting our engineered bacteria. If our product were to be developed and produced in a large-scale, commercial setting, the product will be tested extensively and progressively through the following stages: cell culture, animal models and human trials. The product will be tested for its safety, toxicity, symbiosis and mutagenicity before being certified for use as a health supplement. In the case that the product causes discomfort or irritation to the consumer, they will be advised to stop taking the product and consult a doctor immediately.</span><br /></font></font><span style=""></span><br /><span style=""></span></div></div>
 
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Revision as of 16:40, 17 September 2015

Description - iGEM2015 wiki

The organisms used for this project:

Species Strain no. / name Risk Group Any risk to humans?
K-12 DH5α 1 E. coli K-12 is considered as having no or
low individual and community risk. It is
unlikely to cause human or animal disease.
K-12 JM109 1

General public Safety:
  • All E. coli strains used in this project are attenuated mutant strains that are unlikely to survive/propagate in the environment outside the laboratory as they harbor many genetic mutations in their genome. 
  • To reduce the risk of biological materials escaping from the lab, all bacterial strains are kept and stored in designated refrigerators (before and after all experiments) in the supervisor’s (Dr. Richard Kong) laboratory which is not accessible to the general public. The lab where iGEM experiments are conducted is also not accessible to the general public because all lab workers need to be authorized to work in the lab and are required to carry a “name badge”.

Environmental Safety:
  • Possible risks : Spillage of bacterial cultures or organic wastes/solutions 
  • Solution: Spillages will be immediately dealt with in the lab using appropriate disinfection procedures (e.g. disinfection with antibacterial detergents) and contaminated bench surface will be cleaned with 70% ethanol.
  • Organic solvents, biological materials and wastes (microorganisms) and plastic-wares are collected in separate waste containers for collection by technical staff. Biological wastes are treated with bleach and subsequently autoclaved to kill off all remaining microorganisms.

What new risks might arise from our product growth?
One possible risk is that consumers may suffer from side-effects or complication from ingesting our engineered bacteria. If our product were to be developed and produced in a large-scale, commercial setting, the product will be tested extensively and progressively through the following stages: cell culture, animal models and human trials. The product will be tested for its safety, toxicity, symbiosis and mutagenicity before being certified for use as a health supplement. In the case that the product causes discomfort or irritation to the consumer, they will be advised to stop taking the product and consult a doctor immediately.