Difference between revisions of "Team:Kent/Notebook"

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<html>
  
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<h2>Notebook</h2>
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<h1>Notebook</h1>
  
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<p> Document the dates you worked on your project.</p>
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<h4> Day 1 Wet lab <i>22/06/15</i> </h4>
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<p><li> Made LB plates </li>
 +
      <li> Made LB broth </li>
 +
      <li> TIPS autoclaved </li>
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</p>
  
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<h5>What should this page have?</h5>
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<h4> Day 2 Wet lab <i>23/06/15</i> </h4>
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<ul>
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<p><li> Set up Top10 overnight, VS45 overnight, US348 overnight </li>
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<li>Chronological notes of what your team is doing.</li>
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</p>
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<li> Brief descriptions of daily important events.</li>
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<li>Pictures of your progress. </li>
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<li>Mention who participated in what task.</li>
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</ul>
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 +
<h4> Day 3 Wet lab <i>24/06/15</i></h4>
 +
<p><li> Filter sterilise the buffer</li>
 +
      <li> 250ml no antibiotic broth, put top10 cells in</li>
 +
      <li> Incubate until optical density is 0.6 </li>
 +
      <li> Miniprep PSB1C3 plasmid </li>
 +
</p>
  
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<h4>Inspiration</h4>
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<h4> Day 4 Wet lab <i> 25/06/15 </i> </h4>
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<p>You can see what others teams have done to organize their notes:</p>
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<p> <li> Transformation </li>
 +
      <li> Prepare SBC media </li>
 +
      <li> Digest of PSB1c3 from MS348 </li>
 +
</p>
 +
 
 +
<h4> Day 5 Wet lab <i> 26/06/15 </i> </h4>
 +
<p><li> Calculated competent cell efficiency </li>
 +
      <li> Agarose gel formation </li>
 +
      <li> Gel electrophoresis </li>
 +
</p>
 +
 
 +
<h4> Day 6 Wet lab <i> 29/06/15 </i> </h4>
 +
<p></li> Transforming linear PSBC13 </li>
 +
</p>
  
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<ul>
 
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
 
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
 
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
 
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</ul>
 
  
 
</div>
 
</div>
 
</html>
 
</html>
 
{{KentFooter}}
 
{{KentFooter}}

Revision as of 13:08, 29 June 2015

iGEM Kent 2015


Notebook

Day 1 Wet lab 22/06/15

  • Made LB plates
  • Made LB broth
  • TIPS autoclaved

    • Day 2 Wet lab 23/06/15

  • Set up Top10 overnight, VS45 overnight, US348 overnight

    • Day 3 Wet lab 24/06/15

  • Filter sterilise the buffer
  • 250ml no antibiotic broth, put top10 cells in
  • Incubate until optical density is 0.6
  • Miniprep PSB1C3 plasmid

    • Day 4 Wet lab 25/06/15

  • Transformation
  • Prepare SBC media
  • Digest of PSB1c3 from MS348

    • Day 5 Wet lab 26/06/15

  • Calculated competent cell efficiency
  • Agarose gel formation
  • Gel electrophoresis

    • Day 6 Wet lab 29/06/15

    Transforming linear PSBC13