Difference between revisions of "Team:Kent/Notebook"

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<h1>Notebook</h1>
 
<h1>Notebook</h1>
  
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<h3> Contents </h3>
 
<a href="#week1">Week 1</a> <br>
 
<a href="#week1">Week 1</a> <br>
 
<a href="#week2">Week 2</a>
 
<a href="#week2">Week 2</a>

Revision as of 13:21, 29 June 2015

iGEM Kent 2015


Notebook

Contents

Week 1
Week 2

Week 1

Day 1 Wet lab 22/06/15

  • Made LB plates
  • Made LB broth
  • TIPS autoclaved

    • Day 2 Wet lab 23/06/15

  • Set up Top10 overnight, VS45 overnight, US348 overnight

    • Day 3 Wet lab 24/06/15

  • Filter sterilise the buffer
  • 250ml no antibiotic broth, put top10 cells in
  • Incubate until optical density is 0.6
  • Miniprep PSB1C3 plasmid

    • Day 4 Wet lab 25/06/15

  • Transformation
  • Prepare SBC media
  • Digest of PSB1c3 from MS348

    • Day 5 Wet lab 26/06/15

  • Calculated competent cell efficiency
  • Agarose gel formation
  • Gel electrophoresis

    • Week 2

    Day 6 Wet lab 29/06/15

    Transforming linear PSBC13