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Revision as of 17:07, 17 September 2015
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SAFETY
Laboratory Safety
In our project we worked with BL1 materials E. coli for cloning and BL2 materials Human Embryonic Kidney 293 cells for transfection. All students in our team received lab-specific training and students who worked with BL2 material received additional blood-borne pathogens training. Before we start our experiment, we made a detailed protocol and communicated with our instructor to ensure that we are followed the safety standard in laboratory. Before each experiment we wore protective equipment such as lab coat, gloves and gauze mask. To keep ourselves and our sample safe all the experiments of BL2 materials were handled in biosafety cabinets .After experiment, BL2 material devil liquor was handled with decontamination bleach mixture under ultraviolet rays for 24 hours. All laboratory chemicals were handled in accordance with the information on their MSDS to keep us safe.
Biological Parts
We construct 2 basic parts that encoding internal protein in human body and designed 3 scilencing devices . The most hazardous biological parts in our parts are the scilencing devices since they encode the hairpin RNA that do not exsist in human body and they may cause potential risks. We transfected the scilencing devices into HEK293 to scilence the PDE5A gene. To make our parts safer we designed a hypoxia responsive switch, only under hypoxia situation the down stream parts will work. In our experiment we used lentivirus to packaged our plasmids so that all the transfection experiments were handled in II biological safety cabinet.