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Revision as of 17:31, 17 September 2015

Restriction analyse
Restricion analysis from plasmids
extra integration of DNA in thrC locus
Correct analysis from the plasmids

<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/none">none</a>

00:00, 28 Augustus 2015 - 00:00, 28 Augustus 2015

Restriction analyse miniprep thrC locus 1

To 5 ul from each plasmid was added: 13 ul MiliQ 1 ul fast digest EcoRV 1 ul fast digest buffer incubated for 1 hour at 37C Good plasmid would have 2 bands K823023 (original) or pdg1664 (original) would have 1 band. All 5 plasmids had two bands. One of the plasmids will be used further. named as thrC plasmid A

Marieke