Difference between revisions of "Team:Paris Saclay/Notebook/July/7"
m |
m |
||
| Line 28: | Line 28: | ||
====Electrophoresis==== | ====Electrophoresis==== | ||
| + | [[File:ParisSaclay_070715-digestion_vérif_plasmides.jpg|300px|right]] | ||
''by Johan, Audrey, Pauline'' | ''by Johan, Audrey, Pauline'' | ||
| − | |||
Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET | Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET | ||
Migration 0,06A 80V | Migration 0,06A 80V | ||
Revision as of 19:02, 17 September 2015
Contents
Tuesday 7th July
Labwork
Verification of our plasmids
by Coralie, Johan, Seong Koo, Audrey, Pauline
Biobricks verification
- K115017
- C0040
- I13602
- J23101
- J23117
- J23106
- I13504
Extraction
With the Nucleospin Kit from Macherey Nagel
Digestion by NotI
Stock solution preparation :
- 124µL H2O
- 16µL buffer FastDigest 10x
- 4µL NotI
We use 18µL from this solution with 2µL plasmid solution
Incubation 1h30 at 37°C
Electrophoresis
by Johan, Audrey, Pauline Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET Migration 0,06A 80V
New culture
by Pauline, Audrey
New liquid culture of:
- K1493504
2x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony. We incubate cultures at 37°C, ON.
Members present:
- Instructors and advisors: Alice.
- Students: Johan, Seong Koo, Audrey, Coralie, Pauline
Contact
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. All rights reserved All inspirations encouraged.
*This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. *This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.