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Colonies from the transformation were checked with a colony PCR. Herefore the following primers were used. | Colonies from the transformation were checked with a colony PCR. Herefore the following primers were used. | ||
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AATTCATGTAAAAGATGAGGTTGGTTCATT | AATTCATGTAAAAGATGAGGTTGGTTCATT | ||
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iGEM erm-thrC rev 2 | iGEM erm-thrC rev 2 | ||
AGGAAGTTAAAGGAGCTCGAATTAATTCC | AGGAAGTTAAAGGAGCTCGAATTAATTCC | ||
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Colonies from the transformation were checked with a colony PCR. Herefore the following primers were used.
IGEM thrC forward
AATTCATGTAAAAGATGAGGTTGGTTCATT
iGEM erm-thrC rev 2 AGGAAGTTAAAGGAGCTCGAATTAATTCC
expected bands around 2500 bp. Colony 5 showed the correct band on the agarose gel after the colony PCR. This colony was grown on LB with Ampicillin overnight at 37°C