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| − | <h3>K1602024 - HistidinTag-Green fluorescent protein (His-GFP)</h3>
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| − | <html><div class="contentSection">
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| − | Competent <em>E. coli </em>Top 10 cells were retransformed via heat shock with pSB1C3 harbouring <em> GFP</em> provided by the iGEM Registry <a href=" http://parts.igem.org/Part:BBa_E0040 " title="Opens internal link in current window" class="internal link">(BBa_E0040)</a>. The plasmids were extracted and used as templates for a PCR with oligonucleotides 42 and VR. The PCR product was verified by gel electrophoresis (Figure 1) and digested with <em> Dpn</em>I. The purified product was digested with <em>EcoR</em>I and <em>Pst</em>I and ligated into pSB1C3. Competent <em>E. coli</em> Top 10 cells were transformed via heat shock and colonies were screened by colony PCR with oligonucleotides VF2 and VR (Figure 2). </p>
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| − | <figure class="leftFig" style="width:30%">
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| − | <img src="https://static.igem.org/mediawiki/2015/8/83/Figure_1-_Standard_PCR_of_Prefix-His-Tag-GFP.png" width=80% height=80%>
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| − | <figcaption><br><b>Figure 1</b> Standard PCR of Prefix-His-Tag-GFP (3). The size of the amplified product was around 0.9 kbp. DNA marker: 2-Log DNA Ladder (NEB).</figcaption>
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| − | </figure>
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| − | <figure class="rightFig" style="width:30%">
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| − | <img src="https://static.igem.org/mediawiki/2015/f/f7/Figure_2-_Colony_PCR_of_Prefix-His-Tag-GFP.png" width=80% height=80%>
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| − | <figcaption><br><b>Figure 2</b> : Colony PCR of Prefix-His-Tag-GFP (1+2). Colonies 1 and 2 contained the insert of around 0.9 kbp. DNA marker: 2-Log DNA Ladder (NEB).</figcaption>
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| − | </figure>
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| − | </div></html>
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