Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/Ligation th22"
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Revision as of 20:26, 17 September 2015
Ligation with thrC complete with inserts th22
Ligation of thrC complete plasmid with tasA, bslA or slrR
extra integration of DNA in thrC locus
Ligated products
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Ligation">Ligation</a>
00:00, 6 September 2015 - 00:00, 6 September 2015
The ligation of the thrC with inserts included ligating thrC with the Psalt + tasA and with Psalt + bslA. Finally slrR was also ligated in the thrC backbone. The ligations were incubated for 1 hour at room temperature.