Difference between revisions of "Team:Kent/Notebook"

Line 6: Line 6:
  
 
<h1>Notebook</h1>
 
<h1>Notebook</h1>
 +
 +
<table>
 +
<tr>
 +
<th colspan="100%">
 +
June
 +
</th>
 +
</tr>
 +
<tr>
 +
<td>
 +
1
 +
</td>
 +
<td>
 +
2
 +
</td>
 +
<td>
 +
3
 +
</td>
 +
<td>
 +
4
 +
</td>
 +
<td>
 +
5
 +
</td>
 +
<td>
 +
6
 +
</td>
 +
<td>
 +
7
 +
</td>
 +
<td>
 +
8
 +
</td>
 +
<td>
 +
9
 +
</td>
 +
<td>
 +
10
 +
</td>
 +
<td>
 +
11
 +
</td>
 +
<td>
 +
12
 +
</td>
 +
<td>
 +
13
 +
</td>
 +
<td>
 +
14
 +
</td>
 +
<td>
 +
15
 +
</td>
 +
<td>
 +
16
 +
</td>
 +
<td>
 +
17
 +
</td>
 +
<td>
 +
18
 +
</td>
 +
<td>
 +
19
 +
</td>
 +
<td>
 +
20
 +
</td>
 +
<td>
 +
21
 +
</td>
 +
<td>
 +
22
 +
</td>
 +
<td>
 +
23
 +
</td>
 +
<td>
 +
24
 +
</td>
 +
<td>
 +
25
 +
</td>
 +
<td>
 +
26
 +
</td>
 +
<td>
 +
27
 +
</td>
 +
<td>
 +
28
 +
</td>
 +
<td>
 +
29
 +
</td>
 +
<td>
 +
30
 +
</td>
 +
</tr>
 +
</table>
 +
  
 
<h3> Contents </h3>
 
<h3> Contents </h3>

Revision as of 08:26, 30 June 2015


iGEM Kent 2015

Notebook

June
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Contents

Week 1
Week 2

Week 1

Day 1 Wet lab 22/06/15

  • Made LB plates
  • Made LB broth
  • TIPS autoclaved

    • Day 2 Wet lab 23/06/15

  • Set up Top10 overnight, VS45 overnight, US348 overnight

    • Day 3 Wet lab 24/06/15

  • Filter sterilise the buffer
  • 250ml no antibiotic broth, put top10 cells in
  • Incubate until optical density is 0.6
  • Miniprep PSB1C3 plasmid

    • Day 4 Wet lab 25/06/15

  • Transformation
  • Prepare SBC media
  • Digest of PSB1c3 from MS348

    • Day 5 Wet lab 26/06/15

  • Calculated competent cell efficiency
  • Agarose gel formation
  • Gel electrophoresis

    • Week 2

    Day 6 Wet lab 29/06/15

    Transforming linear PSBC13