Difference between revisions of "Team:Warwick/Parts"

 
(15 intermediate revisions by 4 users not shown)
Line 2: Line 2:
  
 
<html>
 
<html>
<!-- SLIDER
+
 
================================================== -->
+
<div id="ei-slider" class="ei-slider">
+
<ul class="ei-slider-large">
+
+
<li>
+
<a href="#link1"><img src="http://www.blackwallpaper.xyz/wp-content/uploads/2015/07/black-and-white-wallpaper.jpg" alt="image10" class="responsiveslide"></a>
+
</li>
+
</ul>
+
+
</div>
+
<div class="minipause">
+
</div>
+
 
<!-- SUBHEADER
 
<!-- SUBHEADER
 
================================================== -->
 
================================================== -->
Line 28: Line 16:
 
<!-- CONTENT  
 
<!-- CONTENT  
 
================================================== -->
 
================================================== -->
 +
 +
</div>
 +
 +
 
<div class="row">
 
<div class="row">
 
     <!-- MAIN CONTENT-->
 
     <!-- MAIN CONTENT-->
 
<div class="eight columns">
 
<div class="eight columns">
<div class="sectiontitle">
+
<h4>Our Submitted Parts</h4>
+
<div class="sectiontitle">
 +
<h4>Parts</h4>
 +
</div>
 
</div>
 
</div>
 
<!-- Services List-->
 
<!-- Services List-->
<div class="twelve columns noleftmargin">
+
<div class="twelve columns noleftmargin">
<h5 class="sidebartitle">Part 1</h5>
+
 
 +
  Our new parts are all versions of the same modular system, a fusion protein formed from a bacterial transmembrane domain and a zinc-finger domain, with linker domains between domains and a FLAG-tag for immunofluorescence staining. Each transmembrane/zinc-finger domain is independently interchangeable with other transmembrane/zinc-finger domains, though not interchangeable between each other (i.e. the transmembrane domain cannot be swapped for a zinc-finger domain and vice versa).
 +
    <br> The purpose of this fusion protein is to allow bacteria (specifically <i>Escherichia coli</i>) to bind specific short double stranded DNA sequences at their surface; using immobilised DNA the cell should remain bound in place, or this protein can be used for cell identification (barcoding).
 +
 
 +
<p>
 +
<img src="https://static.igem.org/mediawiki/2015/5/5c/Warwick_Gene_Map.jpeg" align="middle">
 +
</p>
 +
 
 +
 
 +
<br>
 +
<p>_______________________________________________________________________________________________________________________________________</p> <h5>Lpp-OmpA-Zif268</h5>
 
<p>
 
<p>
<img src="images/settings.png" class="pics" alt=""> ADD DESCRIPTION...............................................................
+
This part is our construct with Lpp-OmpA as the transmembrane domain and the Zif268 1AAY fragment as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag.
 
</p>
 
</p>
 +
<p>
 +
<a href="http://parts.igem.org/Part:BBa_K1855000">Registry Link</a>
 +
</p>
 
</div>
 
</div>
 
<div class="twelve columns noleftmargin">
 
<div class="twelve columns noleftmargin">
<h5 class="sidebartitle">Part 2</h5>
+
<p>_______________________________________________________________________________________________________________________________________</p> <h5>Lpp-OmpA-sZF2</h5>
 
<p>
 
<p>
<img src="images/settings.png" class="pics" alt=""> ADD DESCRIPTION...............................................................
+
This part is our construct with Lpp-OmpA as the transmembrane domain and sZF2 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag.
 
</p>
 
</p>
 +
<p>
 +
<a href="http://parts.igem.org/Part:BBa_K1855001">Registry Link</a>
 +
</p>
 
</div>
 
</div>
 
<div class="twelve columns noleftmargin">
 
<div class="twelve columns noleftmargin">
<h5 class="sidebartitle">Part 3</h5>
+
<p>_______________________________________________________________________________________________________________________________________</p> <h5>Lpp-OmpA-sZF10</h5>
 
<p>
 
<p>
<img src="images/settings.png" class="pics" alt=""> ADD DESCRIPTION...............................................................
+
This part is our construct with Lpp-OmpA as the transmembrane domain and sZF10 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag.
 
</p>
 
</p>
 +
<p>
 +
<a href="http://parts.igem.org/Part:BBa_K1855002">Registry Link</a>
 +
</p>
 
</div>
 
</div>
 
<div class="twelve columns noleftmargin">
 
<div class="twelve columns noleftmargin">
<h5 class="sidebartitle">Part 4</h5>
+
<p>_______________________________________________________________________________________________________________________________________</p><h5>Lpp-OmpA-sZF14</h5>
 
<p>
 
<p>
<img src="images/settings.png" class="pics" alt=""> ADD DESCRIPTION...............................................................
+
This part is our construct with Lpp-OmpA as the transmembrane domain and sZF14 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag.
 +
 
 
</p>
 
</p>
 +
<p>
 +
<a href="http://parts.igem.org/Part:BBa_K1855003">Registry Link</a>
 +
</p>
 
</div>
 
</div>
<div class="clear">
+
 
 +
 
 +
 
 +
 
 +
<div class="twelve columns noleftmargin">
 +
<p>_______________________________________________________________________________________________________________________________________</p><h5>INP-Zif268</h5>
 +
<p>
 +
This part is our construct with INP as the transmembrane domain and the Zif268 1AAY fragment as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag.
 +
</p>
 +
<p>
 +
<a href="http://parts.igem.org/Part:BBa_K1855004">Registry Link</a>
 +
</p>
 
</div>
 
</div>
<br>
+
 
<div class="panel">
+
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<div class="twelve columns noleftmargin">
 +
<p>_______________________________________________________________________________________________________________________________________</p> <h5>BclA-Zif268
 +
</h5>
 
<p>
 
<p>
<b></b><a href="#menu-toggle" class="btn btn-default" id="menu-toggle">Toggle Menu</a></p>
+
This part is our construct with BclA as the transmembrane domain and the Zif268 1AAY fragment as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag.
 
</p>
 
</p>
 +
<p>
 +
<a href="http://parts.igem.org/Part:BBa_K1855005">Registry Link</a>
 +
</p>
 +
</div>
 +
 +
 +
 +
 +
<div class="clear">
 +
</div>
 +
 +
 
</div>
 
</div>
 
</div><!-- end main content-->
 
</div><!-- end main content-->
Line 71: Line 120:
 
     <!-- SIDEBAR-->
 
     <!-- SIDEBAR-->
 
 
</div>
 
<div class="hr">
 
</div>
 
  
  
<script>$("#menu-toggle").click(function(e) {
 
        e.preventDefault();
 
        $("#wrapper").toggleClass("toggled");
 
    });</script>
 
 
</div>
 
</div>
 
</div>
 
</div>
 
</div>
 
</div>
 
 
  
 
</html>
 
</html>
  
 
{{WarwickFooter}}
 
{{WarwickFooter}}

Latest revision as of 20:40, 17 September 2015

Warwick iGEM 2015