Difference between revisions of "Team:Warwick/Parts"
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Our new parts are all versions of the same modular system, a fusion protein formed from a bacterial transmembrane domain and a zinc-finger domain, with linker domains between domains and a FLAG-tag for immunofluorescence staining. Each transmembrane/zinc-finger domain is independently interchangeable with other transmembrane/zinc-finger domains, though not interchangeable between each other (i.e. the transmembrane domain cannot be swapped for a zinc-finger domain and vice versa). | Our new parts are all versions of the same modular system, a fusion protein formed from a bacterial transmembrane domain and a zinc-finger domain, with linker domains between domains and a FLAG-tag for immunofluorescence staining. Each transmembrane/zinc-finger domain is independently interchangeable with other transmembrane/zinc-finger domains, though not interchangeable between each other (i.e. the transmembrane domain cannot be swapped for a zinc-finger domain and vice versa). | ||
| − | <br> The purpose of this fusion protein is to allow bacteria (specifically Escherichia coli) to bind specific short double stranded DNA sequences at their surface; using immobilised DNA the cell should remain bound in place, or this protein can be used for cell identification (barcoding). | + | <br> The purpose of this fusion protein is to allow bacteria (specifically <i>Escherichia coli</i>) to bind specific short double stranded DNA sequences at their surface; using immobilised DNA the cell should remain bound in place, or this protein can be used for cell identification (barcoding). |
| + | |||
| + | <p> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/5/5c/Warwick_Gene_Map.jpeg" align="middle"> | ||
| + | </p> | ||
<br> | <br> | ||
| − | + | <p>_______________________________________________________________________________________________________________________________________</p> <h5>Lpp-OmpA-Zif268</h5> | |
<p> | <p> | ||
This part is our construct with Lpp-OmpA as the transmembrane domain and the Zif268 1AAY fragment as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | This part is our construct with Lpp-OmpA as the transmembrane domain and the Zif268 1AAY fragment as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | ||
</p> | </p> | ||
| + | <p> | ||
| + | <a href="http://parts.igem.org/Part:BBa_K1855000">Registry Link</a> | ||
| + | </p> | ||
</div> | </div> | ||
<div class="twelve columns noleftmargin"> | <div class="twelve columns noleftmargin"> | ||
| − | + | <p>_______________________________________________________________________________________________________________________________________</p> <h5>Lpp-OmpA-sZF2</h5> | |
<p> | <p> | ||
This part is our construct with Lpp-OmpA as the transmembrane domain and sZF2 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | This part is our construct with Lpp-OmpA as the transmembrane domain and sZF2 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | ||
</p> | </p> | ||
| + | <p> | ||
| + | <a href="http://parts.igem.org/Part:BBa_K1855001">Registry Link</a> | ||
| + | </p> | ||
</div> | </div> | ||
<div class="twelve columns noleftmargin"> | <div class="twelve columns noleftmargin"> | ||
| − | + | <p>_______________________________________________________________________________________________________________________________________</p> <h5>Lpp-OmpA-sZF10</h5> | |
<p> | <p> | ||
This part is our construct with Lpp-OmpA as the transmembrane domain and sZF10 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | This part is our construct with Lpp-OmpA as the transmembrane domain and sZF10 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | ||
</p> | </p> | ||
| + | <p> | ||
| + | <a href="http://parts.igem.org/Part:BBa_K1855002">Registry Link</a> | ||
| + | </p> | ||
</div> | </div> | ||
<div class="twelve columns noleftmargin"> | <div class="twelve columns noleftmargin"> | ||
| − | <h5 | + | <p>_______________________________________________________________________________________________________________________________________</p><h5>Lpp-OmpA-sZF14</h5> |
<p> | <p> | ||
This part is our construct with Lpp-OmpA as the transmembrane domain and sZF14 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | This part is our construct with Lpp-OmpA as the transmembrane domain and sZF14 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | ||
</p> | </p> | ||
| + | <p> | ||
| + | <a href="http://parts.igem.org/Part:BBa_K1855003">Registry Link</a> | ||
| + | </p> | ||
</div> | </div> | ||
| Line 65: | Line 81: | ||
<div class="twelve columns noleftmargin"> | <div class="twelve columns noleftmargin"> | ||
| − | <h5 | + | <p>_______________________________________________________________________________________________________________________________________</p><h5>INP-Zif268</h5> |
<p> | <p> | ||
This part is our construct with INP as the transmembrane domain and the Zif268 1AAY fragment as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | This part is our construct with INP as the transmembrane domain and the Zif268 1AAY fragment as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | ||
</p> | </p> | ||
| + | <p> | ||
| + | <a href="http://parts.igem.org/Part:BBa_K1855004">Registry Link</a> | ||
| + | </p> | ||
</div> | </div> | ||
| Line 79: | Line 98: | ||
<div class="twelve columns noleftmargin"> | <div class="twelve columns noleftmargin"> | ||
| − | + | <p>_______________________________________________________________________________________________________________________________________</p> <h5>BclA-Zif268 | |
| + | </h5> | ||
<p> | <p> | ||
| − | + | This part is our construct with BclA as the transmembrane domain and the Zif268 1AAY fragment as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | |
</p> | </p> | ||
| + | <p> | ||
| + | <a href="http://parts.igem.org/Part:BBa_K1855005">Registry Link</a> | ||
| + | </p> | ||
</div> | </div> | ||
Latest revision as of 20:40, 17 September 2015
