Difference between revisions of "Team:Warwick/Parts"

 
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<h4>Studio Francesca</h4>
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</ul><select id="tinynav1" class="tinynav tinynav1"><option>MENU</option><option value="index.html">Home</option><option value="index2.html">- Without slider</option><option value="#">Portofolio</option><option value="portofolio2.html">- 2 Columns</option><option value="portofolio3.html">- 3 Columns</option><option value="portofolio4.html">- 4 Columns</option><option value="portofoliodetail.html">- Project Details</option><option value="#">Blog</option><option value="blogpage1.html">- Blog page style 1</option><option value="blogpage2.html">- Blog page style 2</option><option value="blogpage3.html">- Blog page style 3</option><option value="blogsinglepost.html">- Single post</option><option value="#">Pages</option><option value="about.html">- About us</option><option value="services.html">- Services</option><option value="#">Features</option><option value="columns.html">- Columns</option><option value="elements.html">- Elements</option><option value="typography.html">- Typography</option><option value="contact.html">Contact</option></select>
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<h4>Parts</h4>
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<h5>Dawncin'</h5>
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<p class="small date">
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Posted on: 2 Oct 2013 <span class="has-tip tip-top" data-width="90" title="6 comments"><img src="images/comments.png" alt=""></span>
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<img src="images/temp/nivo3.jpg" alt="" class="thumb">"ARE you comin' to the dawncin', Lady Speedway?" asked the American in his best transatlantic liner accent. "Most decidedly not!" Mind you, this answer from Lady Speedway meant red lights ahead. At the Hotel Biscuit...
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<h5> Field Marshal</h5>
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Posted on: 3 Oct 2013 <span class="has-tip tip-top" data-width="90" title="6 comments"><img src="images/comments.png" alt=""></span>
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<img src="images/temp/folio3.jpg" alt="" class="thumb"> She was the Field Marshal of the Front Porch Knitting Needle Hussars, nicknamed "Hussies." Her approbation was olive oil; her discountenance prickly heat. "Of course," she added, "while recognizing that expatiation  ...
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<h5>Lady Speedway</h5>
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Posted on: 2 Oct 2013 <span class="has-tip tip-top" data-width="90" title="6 comments"><img src="images/comments.png" alt=""></span>
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<img src="images/temp/folio4.jpg" alt="" class="thumb">"ARE you comin' to the dawncin', Lady Speedway?" asked the American in his best transatlantic liner accent. "Most decidedly not!" Mind you, this answer from Lady Speedway meant red lights ahead. At the Hotel Biscuit ...
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<h5>Hotel Biscuit</h5>
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Posted on: 3 Oct 2013 <span class="has-tip tip-top" data-width="90" title="6 comments"><img src="images/comments.png" alt=""></span>
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<img src="images/temp/folio6.jpg" alt="" class="thumb"> She was the Field Marshal of the Front Porch Knitting Needle Hussars, nicknamed "Hussies." Her approbation was olive oil; her discountenance prickly heat. "Of course," she added, "while recognizing that expatiation  ...
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<h5>Private Theater</h5>
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Posted on: 2 Oct 2013 <span class="has-tip tip-top" data-width="90" title="6 comments"><img src="images/comments.png" alt=""></span>
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<img src="images/temp/folio3.jpg" alt="" class="thumb">"ARE you comin' to the dawncin', Lady Speedway?" asked the American in his best transatlantic liner accent. "Most decidedly not!" Mind you, this answer from Lady Speedway meant red lights ahead. At the Hotel Biscuit ...
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<h5>Transatlantic</h5>
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Posted on: 3 Oct 2013 <span class="has-tip tip-top" data-width="90" title="6 comments"><img src="images/comments.png" alt=""></span>
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<img src="images/temp/nivo3.jpg" alt="" class="thumb"> She was the Field Marshal of the Front Porch Knitting Needle Hussars, nicknamed "Hussies." Her approbation was olive oil; her discountenance prickly heat. "Of course," she added, "while recognizing that expatiation  ...
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<li class="arrow unavailable"><a href="">«</a></li>
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<li class="current"><a href="">1</a></li>
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<h6 class="sidebartitle">RECENT COMMENTS</h6>
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  Our new parts are all versions of the same modular system, a fusion protein formed from a bacterial transmembrane domain and a zinc-finger domain, with linker domains between domains and a FLAG-tag for immunofluorescence staining. Each transmembrane/zinc-finger domain is independently interchangeable with other transmembrane/zinc-finger domains, though not interchangeable between each other (i.e. the transmembrane domain cannot be swapped for a zinc-finger domain and vice versa).
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    <br> The purpose of this fusion protein is to allow bacteria (specifically <i>Escherichia coli</i>) to bind specific short double stranded DNA sequences at their surface; using immobilised DNA the cell should remain bound in place, or this protein can be used for cell identification (barcoding).
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Many pieces were proposed, but none seemed suitable, because each one wished to play a good ...
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<img src="https://static.igem.org/mediawiki/2015/5/5c/Warwick_Gene_Map.jpeg" align="middle">
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<p>_______________________________________________________________________________________________________________________________________</p> <h5>Lpp-OmpA-Zif268</h5>
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<p>
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This part is our construct with Lpp-OmpA as the transmembrane domain and the Zif268 1AAY fragment as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag.
 
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<img src="images/temp/author2.png" alt="">
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<a href="http://parts.igem.org/Part:BBa_K1855000">Registry Link</a>
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<div class="twelve columns noleftmargin">
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<p>_______________________________________________________________________________________________________________________________________</p> <h5>Lpp-OmpA-sZF2</h5>
 
<p>
 
<p>
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Many pieces were proposed, but none seemed suitable, because each one wished to play a good ...
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This part is our construct with Lpp-OmpA as the transmembrane domain and sZF2 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag.
 
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</p>
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<p>
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<img src="images/temp/author3.png" alt="">
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<a href="http://parts.igem.org/Part:BBa_K1855001">Registry Link</a>
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<div class="twelve columns noleftmargin">
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<p>_______________________________________________________________________________________________________________________________________</p> <h5>Lpp-OmpA-sZF10</h5>
 
<p>
 
<p>
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Many pieces were proposed, but none seemed suitable, because each one wished to play a good ...
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This part is our construct with Lpp-OmpA as the transmembrane domain and sZF10 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag.
 
</p>
 
</p>
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<p>
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<h6 class="sidebartitle">POPULAR TAGS</h6>
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<a href="http://parts.igem.org/Part:BBa_K1855002">Registry Link</a>
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<a href="#" class="tags">Photography</a>
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</p>
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<a href="#" class="tags">Vintage</a>
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<a href="#" class="tags">Nature</a>
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<a href="#" class="tags">Design</a>
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<p>_______________________________________________________________________________________________________________________________________</p><h5>Lpp-OmpA-sZF14</h5>
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<a href="#" class="tags">Printing</a>
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<a href="#" class="tags">Contemporary</a>
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<a href="#" class="tags">Classic</a>
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<a href="#" class="tags">Elegant</a>
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<h6 class="sidebartitle">POPULAR POSTS</h6>
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<p>
 
<p>
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7 Oct 2013
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This part is our construct with Lpp-OmpA as the transmembrane domain and sZF14 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag.
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<a href="http://parts.igem.org/Part:BBa_K1855003">Registry Link</a>
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<p>_______________________________________________________________________________________________________________________________________</p><h5>INP-Zif268</h5>
 
<p>
 
<p>
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Many pieces were proposed, but none seemed suitable, because each one wished to play a good and suitable part ...
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This part is our construct with INP as the transmembrane domain and the Zif268 1AAY fragment as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag.
 
</p>
 
</p>
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<a href="http://parts.igem.org/Part:BBa_K1855004">Registry Link</a>
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<p>_______________________________________________________________________________________________________________________________________</p> <h5>BclA-Zif268
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</h5>
 
<p>
 
<p>
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6 Sept 2013
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This part is our construct with BclA as the transmembrane domain and the Zif268 1AAY fragment as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag.
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It was ready for production, when through accident we were disappointed about securing the private theatre on ...
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4 Aug 2013
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The over-kind praise which it won encouraged me to have the play printed. I decided, as I could see no other prospects...
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<a href="http://parts.igem.org/Part:BBa_K1855005">Registry Link</a>
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