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Revision as of 20:49, 17 September 2015
PCR ferritin (fr1)
Amplifying ferritin
development of ferritin
Amplified ferritin
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/PCR">PCR</a>
{{Team:Groningen/TEMPLATES/LOG |title=00:00, 31 Augustus 2015 - 00:00, 31 Augustus 2015 |executor=Harm Ruesink |content=
Ferritin sequence received from TecCEM HS and ordered at IDT.
PCR ferritin sequence with prefix and suffix primers (figure 1) using NEB Q5 High-Fidelity 2X Master Mix.
Primer
Sequence
prefix
CAGGCAGTTGAATTCGCGGCCGCTTCTAGA
suffix
CTTGAGCTCCTGCAGCGGCCGCTACTAGTA
Primers for ferritin PCR.