Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/Ligation fr3"
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Revision as of 21:09, 17 September 2015
Ligation ferritin in pSB1C3 (fr3)
Ligation ferritin in pSB1C3
extra integration of DNA in thrC locus
Ligated product
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Ligation">Ligation</a>
20:15, 31 August 2015 - 22:30, 10 August 2015
Ferritin dig was ligated into pSB1C3 dig. Ligated at room temperature for 2 hours.
Component
Amount
Ferritin DNA
10 µL
pSB1C3 DNA
10 µL
T4 ligase
0.5 µL
T4 ligase buffer
3 µL
\( \mathrm{H_2O}\)
6.5 µL
Components used for ligation.