Difference between revisions of "Team:Toulouse/Attributions"

The iGEM competition is an enriching experience in our student life, and thanks to this competition we gained a lot of important skills. Indeed, we needed to find sponsors and became experts at phone calling. We also needed to communicate about our project and learned to make visiting cards, newsletters, and to be at explaining about our project and synthetic biology to the general public. Moreover we had to get in touch with different people such as administrators, scientists, beekeepers, specialists, an ethical expert and other iGEM teams and learned a lot through these interactions.

In this part, we want to thank all the persons who contributed to our project by sharing their experience, their knowledge, and their interest.

Our thanks to:

M. Brice Enjalbert for leading the project, for his advice and his availability.

All our advisors who helped us develop our ideas during the brainstorming, and volunteered their knowledge of synthetic biology, microbiology...

The LISBP that gave us full access to the lab and the material we needed, allowing us to work under very good conditions all through the summer.

Our sponsors, for the financial support that enabled us to give reality to our project.

M. Thierry Dufresne (Observatoire Français d’Apidologie), and M. Yves Leconte (INRA Avignon) for all their references in the field of beekeeping, bees and varroas.

M. Vincent Grégoire-Delory and Simon Desbois who were with us throughout our ethical thinking process and thus helped us build the foundations of our project.

To implement our project, we needed to understand the functioning of a beehive and the bees behaviour. Christian Boucher is a researcher from a famous French lab and has some beehives. He agreed to meet us and was able to inform us about bees and to advise us on the molecular biology part of our project. He was a helpful link between bees and biology, and we thank him for his availability.

We owe M. Gilles Truan our deepest gratitude for his availability, and his unreserved support on synthetic biology and genetic regulation.

To analyse the supernatant of our cultures (and particularly to try to detect our compounds of interest), we used the Nuclear Magnetic Resonance (NMR) of the Metatoul platform. We would like to thank them warmly for making this state-of-the-art technology available to us.
Also thanks to Mrs Lindsay Peyriga, who showed our team members how to correctly prepare NMR samples, how to run acquisitions and helped them with the interpretation of the results.

Our thanks to Mrs Hélène Baron, representative of Midi-Pyrénées region in Brussels, and M. Claudes Maranges, who helped us by giving us new lines of search for sponsoring.

We would like to address our special thanks to all the researchers who gave us crucial strains and plasmids for our ApiColi project. All the strains were Escherichia coli strains:

• Gilles Truan (LISBP-Unit EAD11): DH5 alpha
• Stéphanie Heux (INSA/CNRS 5504 - UMR INSA/INRA 792): BW25, MG1663, and two strains with an acetate deletion : Delta pta and Delta ackA (keio strain)
• Kaymeuang Cam (UPS/CNRS UMR5089): MG1655 and MC1661
• Mathieu Fournié (LISBP- Unit EAD11): K823022

We thank Marie Bouvier and Carine Pagès from the “Laboratoire de Microbiologie et Génétique Moléculaires” who kindly gave us plasmids, which unfortunately we did not use.

Mrs Florence Bordes helped us with her high spirits and technical advice during all the project.
M. Thomas Lautier helped and gave us advice during the brainstorming and then particularly for two weeks when all the lab was closed because of holidays.
Mrs Fayza Daboussi, who can be counted on during lab holidays to help and motivate us.
M. Yves Dutruy and Mrs Sylvie Cancel, the lab technicians, who gave us material and advice.