Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/Ligation fr3"
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Revision as of 21:16, 17 September 2015
Digestion ferritin in pSB1C3 (fr3)
restriction of ferritin
Development ferritin
restricted product
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>
16:00, 31 August 2015 - 18:30, 10 August 2015
Digest ferritin and pSB1C3 in order to ligate them. Samples were digested for 2h at 37°C.
#
Component
Amount
1
ferritin
10 µL
10x buffer (2.1)
2 µL
PstI
1 µL
EcoRI
1 µL
\( \mathrm{H_2O}\)
6 µL
2
pSB1C3
10 µL
10x buffer (2.1)
2 µL
PstI
1 µL
EcoRI
1 µL
\( \mathrm{H_2O}\)
6 µL
Components for digestion.