Difference between revisions of "Team:Paris Saclay/Notebook/August/1"

Latest revision as of 22:25, 17 September 2015

Notebook

Our team was relaxing this day, please go back to the calendar.

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-=Monday 3rd August=
+{{Team:Paris_Saclay/notebook_empty}}
-==Lab Work==
-=== New culture===
-'' by Pauline''
-Biobricks:
-* BBa_K1707008
-* BBa_I13600 (2014)
-clones of each in 5mL LB + 20µg/mL of chloramphenicol
-Incubation at 37°C, 250 rpm
-===Plasmid extraction===
-''by Seong-Koo''
-Biobricks:
-* BBa_K1707011 #1 to #6
-* BBa_K1707021 #1 to #6
-* BBa_K1707004 #1 to #6
-Plasmid pIJ773
-With the Macherey-Nagel Extraction kit
-===Soil experiment===
-''by Coralie''
-====Soil====
-Observation of plates (31/07/2015):
-* Soil + H2O on new MCK:  lots of colonies are visible
-* Soil + LB on new MCK: lots of colonies are visible
-* LB on new MCK: nothing
-* MCK alone: nothing
-We can suppose two different hypothesis:
-* The soil is contaminated
-* the MCK isn't OK for our experiment: now we add lactose in the preparation. We will try with sorbitol instead of lactose.
-We try soil + H2O and soil +LB on:
-* MCK + Sorbitol: Without antibiotics, with Spectinomycine, Tetramycine or Chloramphenicol
-* MCK + Lactose: Without antibiotics, with Spectinomycine, Tetramycine or Chloramphenicol
-Incubation ON, 37°C
-===Rehydratation===
-''by Pauline''
-Biobrick: BBa_I13600 (2014-3-19C and 2013-3-20C)
-===Transformation===
-''by Pauline''
-Biobricks:
-* BBa_I13600 (2013, 2014 and 2015)
-* BBa_K1707008
-As usual
-===Plasmid extraction===
-''by Seong-Koo''
-Biobricks:
-* BBa_E0422 #1 and #2
-* BBa_E0022 #1 and #2
-With the Macherey-Nagel Extraction kit
-===New culture===
-''by Pauline''
-Biobricks:
-* BBa_K1707004: amicillin
-* BBa_K1707011
-* BBa_K1707021
-clones of each in 5 mL LB
-===Digestion Verification===
-''by Coralie''
-* Biobricks:
-** BBa_K1707012 #1, #2, #3 and #4
-** BBa_K1707013 #1, #2, #3 and #4
-** BBa_K1707019 #1, #2, #3 and #4
-* Mix:
-** 1 µL Buffer FastDigest 10x
-** 0,5 µL XbaI
-** 0,5 µL PstI
-** 2 µL Plasmid
-** 6 µL H2O
-Incubation 2h, 37°C
-===Electrophoresis===
-''by Coralie''
-Agarose gel, 1%
-Migration 100V
-We can conclude that all biobricks are OK
-===Soil experiment===
-''by Coralie and Johan''
-====Soil====
-Observation of J0 plates: there are a lot of colony in each.
-Control (-):
-* MCK + Tet: 40 pink colonies
-* MCK + Cm: 10 pink colonies
-* MCK + Spectino: 1 pink colony
-* MCK: 100 pink colonies and a lot of white colonies
-We can suppose that we have a contamination between our experiment and control. Or maybe we have a MCK problem because we change it during the experiment.
-So, we decide to stop the experiment and do some test:
-* Soil + H2O on new MCK
-* Soil + LB on new MCK
-* LB on new MCK
-* MCK alone
-Incubation over night, 37°C
-=== New culture===
-'' by Pauline''
-Biobricks:
-* BBa_K1707005
-* BBa_I13602
-A plasmid: PIJ773
-Incubation at 37°C, 250 rpm
-'''Member present:'''
-* Instructors: Alice
-* Students: Coralie, Audrey, Pauline, Johan and Seong-Koo
-{{Team:Paris_Saclay/notebook_footer}}