Difference between revisions of "Team:Paris Saclay/Notebook/August/8"

Latest revision as of 22:27, 17 September 2015

Our team was relaxing this day, please go back to the calendar.

@@ Line 1: / Line 1: @@
-=Monday 8th August=
+{{Team:Paris_Saclay/notebook_empty}}
-==Lab Work==
-===Plasmid extraction===
-''by Pauline''
-Biobrick: BBa_R0051 #1
-With Macherey-Nagel kit
-===Digestion===
-''by Coralie''
-Biobricks: BBa_S03518 #1 and BBa_K1707006 #1
-Mix:
-* 1 µL XbaI
-* 1 µL PstI
-* 2 µL Buffer FastDigest 10x
-* 6 µL H2O
-* 10 µL Plasmid
-Biobricks: BBa_K1707011 and BBa_K1707012
-Mix:
-* 1 µL XbaI
-* 1 µL PstI
-* 2 µL Buffer FastDigest 10x
-* 1 µL H2O
-* 15 µL Plasmid
-Biobricks: BBa_R0040 and BBa_I13602
-Mix:
-* 1 µL XbaI
-* 1 µL EcoRII
-* 2 µL Buffer FastDigest 10x
-* 6 µL H2O
-* 10 µL Plasmid
-Biobricks: BBa_R0051
-Mix:
-* 1 µL SpeI
-* 2 µL PstI
-* 2 µL Buffer Tango 10x
-* 5 µL H2O
-* 10 µL Plasmid
-Biobricks: BBa_K1707004 #1 and #2
-Mix:
-* 1 µL SpeI
-* 2 µL Buffer Tango 10x
-* 2 µL H2O
-* 15 µL Plasmid
-Incubation 1h30, 37°C
-Then, we add to BBa_K1707004 mixes:
-* 3 µL Tango Buffer 10x
-* 1 µL EcoRI
-* 1 µL H2O
-Incubation 1h30, 37°C
-===Purification agarose gel===
-''by Pauline and Coralie''
-Biobricks:
-* BBa_K1707004 #1 and #2
-* BBa_K1707006
-* BBa_K1707011
-* BBa_K1707012
-* BBa_S03518
-'''Member present:'''
-* Instructors: Claire
-* Students: Coralie and Pauline
-{{Team:Paris_Saclay/notebook_footer}}