Difference between revisions of "Team:Austin UTexas"

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<font color="000000">Our iGEM team at the University of Texas at Austin developed five individual projects inspired by members’ interests and concerns in synthetic biology, with a foundation of technical skills and lab experience built during a spring semester course. The projects our team members have devised focus on a multitude of topics, from attempts at improving the stability and efficiency of existing genetic machines, to identifying bacterial factories that can have ecological function. Our projects focused on improving and expanding on the existing microbial factories in E. coli include an attempt to optimize the ΔguaB pDCAF strain of <i>E. coli</i> (Quandt <i>et al.</i>, 2013) to discount nutrients provided by non-caffeine methylxanthines, and a project assessing the evolutionary stability of yellow fluorescent protein, enhanced yellow fluorescent protein, and super-folder yellow fluorescent protein. </font><br>
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<font color="000000">The 2015 UT Austin iGEM Team's major project is primarily concerned with the stability of genetically modified devices in bacteria. Devices run the risk of breaking, or mutating, if they prove to be too much metabolic stress to an organism. We endeavored to identify and better characterize the types of sequences that are prone to breaking, and used fluorescent-protein coding strains of <i>E. coli</i> to observe breaking speed.</font><br>
 
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[[Image:2015_Austin_UTexas_homepage-BB.png|200px|thumb|left|<font size="2">Fluorescent protein-producing bacteria cultures grown over 10 days. One culture was grown each day by moving a small sample of the previous day's culture into new LB media.</font>]]   
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redesign for greater stability<br>
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We constructed plasmids that are capable of degrading different methylxanthines and allowing us to accurately measure their concentrations. The 2012 UT Austin iGEM team, with the help of Dr. Erik Quandt, developed the ΔguaB pDCAF3 strain that could measure the concentration of caffeine by degrading it into a viable replacement for guanine. The 2015 team modified the pDCAF3 strain into seven plasmids that could degrade different methylxanthines with fairly high specificity.<br>
 
<a href="https://2015.igem.org/Team:Austin_UTexas/Project/Caffeine"><font color="017e70"><b>GO TO CAFFEINE PAGE</b></font></a>
 
<a href="https://2015.igem.org/Team:Austin_UTexas/Project/Caffeine"><font color="017e70"><b>GO TO CAFFEINE PAGE</b></font></a>
 
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<b>References</b></br>
 
<b>References</b></br>
 
<h6>Quandt, Erik M., et al. "Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5." ACS synthetic biology 2.6 (2013): 301-307.</br></h6>
 
<h6>Quandt, Erik M., et al. "Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5." ACS synthetic biology 2.6 (2013): 301-307.</br></h6>
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<p><h3><font face="Courier New">INTERLAB STUDY & BREAKING IS BAD</font></h3></p>
 
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what does BB have to do with interlab?
 
 
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<a href="https://2015.igem.org/Team:Austin_UTexas/Interlab_Study"><font color="017e70"><b>GO TO INTERLAB STUDY PAGE</b></font></a>
 
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Revision as of 23:16, 17 September 2015

UT Austin iGEM 2015 Home

BREAKING IS BAD


The 2015 UT Austin iGEM Team's major project is primarily concerned with the stability of genetically modified devices in bacteria. Devices run the risk of breaking, or mutating, if they prove to be too much metabolic stress to an organism. We endeavored to identify and better characterize the types of sequences that are prone to breaking, and used fluorescent-protein coding strains of E. coli to observe breaking speed.

2015 Austin UTexas homepage-BB.png

GO TO PROJECT PAGE


CAFFEINATED COLI


2015 Austin UTexas homepage-caffeine.png

We constructed plasmids that are capable of degrading different methylxanthines and allowing us to accurately measure their concentrations. The 2012 UT Austin iGEM team, with the help of Dr. Erik Quandt, developed the ΔguaB pDCAF3 strain that could measure the concentration of caffeine by degrading it into a viable replacement for guanine. The 2015 team modified the pDCAF3 strain into seven plasmids that could degrade different methylxanthines with fairly high specificity.
GO TO CAFFEINE PAGE


References

Quandt, Erik M., et al. "Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5." ACS synthetic biology 2.6 (2013): 301-307.