Difference between revisions of "Team:CHINA CD UESTC/Collaborations"

 
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&nbsp;&nbsp;This is the list of contributions and the use of resources of our all team members throughout the whole iGEM competition. In brief, our team is composed of 11 undergraduates and 4 instructors who are together to complete the project. What we represent about our entire final results all come from our 11 students. Hope this page can provide you with a clear message.
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&nbsp;&nbsp;This year the collaboration of our team with SCU_China team and UESTC-software team were very smoothly as expected. We helped each other from the aspects of experimental design, experiment results verification and software to reach the iGEM spirit of resource sharing. The following is a detailed description.
 
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In 2015 iGEM，we UESTC teams helped <strong>SCU_China</strong> to finish the prediction and experimental demonstration about RBS in their project.</p>
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In 2015 iGEM, we CHINA_CD_UESTC team collaborated with SCU_China team and UESTC-software team. We communicated several times, helped each other and shared our resources, which generated a great atmosphere of cooperation and mutual assistance. Indeed, we helped SCU_China team finish the prediction and experiment design about RBS in their project.
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<h4>Lab team: </h4>
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<P>CHINA_CD_UESTC designed the experiment and finished the demonstration.</P>
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<h4>Software team:</h4>
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<p><a href="https://2015.igem.org/Team:UESTC_Software/Collaborations.html">UESTC_Software</a> created a software named <a href="http://cefg.cn/Igem2015/mccap-server/app/tools/sdFinder.php">SD Finder</a>.</p>
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First about the prediction: CHINA_CD_UESTC offered technology and method. The software team UESTC_Software program and finish the software. The two teams from UESTC helped SCU_China to create a software named <a href="http://cefg.cn/Igem2015/mccap-server/app/tools/sdFinder.php">SD Finder</a> in helping user predict the ORF and SD sequence of DNA sequence.  
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<p>After finishing the prediction, we CHINA_CD_UESTC helped SCU_China to design the experiment and finish the demonstration. In order to find the unknown RBS position and standard it , we designed this experiment.</p>
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<p>In this process, our team had completed two aspects of the collaboration work:</p>
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<h5>(1) Help SCU_China team to design the experiments and complete the experimental demonstration of RBS forecast outcomes.</h5>
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<h5>(2) Provide the theoretical support of developing SD Finder software aiming to predict the ribosome binding site (RBS) position to UESTC-software team.</h5>
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<h2>Design experiments for SCU_China team</h2>
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We helped SCU_China team design the experiment. In order to find the unknown RBS position and standard it, we designed the folowing experiments for them:
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<p id="pic_title">1. Demonstration of RBS located in upstream of the gene <i>nifD</i></p>
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<img src="https://static.igem.org/mediawiki/2015/e/ea/CHINA_CD_UESTC_COLLABORATION01.png" width="60%">
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<p id="pic_title">2. Mutant demonstration of RBS located in upstream of the gene <i>nifD</i></p>
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<img src="https://static.igem.org/mediawiki/2015/8/8b/CHINA_CD_UESTC_COLLABORATION02.png" width="60%">
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<p id="pic_title">3. Demonstration of RBS located in upstream of the gene <i>nifK</i></p>
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<img src="https://static.igem.org/mediawiki/2015/7/79/CHINA_CD_UESTC_COLLABORATION03.png" width="60%">
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<div class="project_pic">
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<p id="pic_title">4. Mutant demonstration of RBS located in upstream of the gene <i>nifK</i></p>
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<img src="https://static.igem.org/mediawiki/2015/8/84/CHINA_CD_UESTC_COLLABORATION04.png" width="60%">
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<p>CHINA_CD_UESTC team finally completed the design of these experiments, which helped SCU_China team accomplish the work of forecast RBS position.</p>
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<h2>SD Finder Theoretical Support</h2>
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It was in our theoretical knowledge support that UESTC-software team helped SCU_China team develop a RBS prediction software which named SD Finder. The two teams, CHINA_CD_UESTC team and UESTC-software team, made up deficiencies in theoretical support and software technology to each other and cooperated to complete the SD Finder smoothly. The main function of SD Finder is helping user predict the ORF and SD sequence of DNA sequence. Software links below:
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<a href="http://cefg.cn/Igem2015/mccap-server/app/tools/sdFinder.php">
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<p>http://cefg.cn/Igem2015/mccap-server/app/tools/sdFinder.php</p>
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<img src="https://static.igem.org/mediawiki/2015/6/68/CHINA_CD_UESTC_COLLABORATION06.png" width="80%" height="80%"></div>
 
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<h2>1. Demonstration of RBS located in nifD up stream</h2>
 
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<h2>2. Mutant demonstration of RBS located in nifD up stream</h2>
 
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<img src="https://static.igem.org/mediawiki/2015/f/fb/CD_UESTC_Hz.jpg" width="80%" height="80%">
 
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<h2>3. Demonstration of RBS located in nifK up stream</h2>
 
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<h2>4. Mutant demonstration of RBS located in nifK up stream</h2>
 
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<img src="https://static.igem.org/mediawiki/2015/f/fb/CD_UESTC_Hz.jpg" width="80%" height="80%">
 
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<p>We finally finished this experiment and helped SCU_China a lot. Both schools enjoyed this cooperation.</p>
 
 
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Latest revision as of 00:33, 18 September 2015


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COLLABORATIONS

  This year the collaboration of our team with SCU_China team and UESTC-software team were very smoothly as expected. We helped each other from the aspects of experimental design, experiment results verification and software to reach the iGEM spirit of resource sharing. The following is a detailed description.

In 2015 iGEM, we CHINA_CD_UESTC team collaborated with SCU_China team and UESTC-software team. We communicated several times, helped each other and shared our resources, which generated a great atmosphere of cooperation and mutual assistance. Indeed, we helped SCU_China team finish the prediction and experiment design about RBS in their project.

In this process, our team had completed two aspects of the collaboration work:

  • (1) Help SCU_China team to design the experiments and complete the experimental demonstration of RBS forecast outcomes.
  • (2) Provide the theoretical support of developing SD Finder software aiming to predict the ribosome binding site (RBS) position to UESTC-software team.

Design experiments for SCU_China team

We helped SCU_China team design the experiment. In order to find the unknown RBS position and standard it, we designed the folowing experiments for them:

1. Demonstration of RBS located in upstream of the gene nifD

2. Mutant demonstration of RBS located in upstream of the gene nifD

3. Demonstration of RBS located in upstream of the gene nifK

4. Mutant demonstration of RBS located in upstream of the gene nifK

CHINA_CD_UESTC team finally completed the design of these experiments, which helped SCU_China team accomplish the work of forecast RBS position.

SD Finder Theoretical Support

It was in our theoretical knowledge support that UESTC-software team helped SCU_China team develop a RBS prediction software which named SD Finder. The two teams, CHINA_CD_UESTC team and UESTC-software team, made up deficiencies in theoretical support and software technology to each other and cooperated to complete the SD Finder smoothly. The main function of SD Finder is helping user predict the ORF and SD sequence of DNA sequence. Software links below:

http://cefg.cn/Igem2015/mccap-server/app/tools/sdFinder.php