Difference between revisions of "Team:CHINA CD UESTC/Collaborations"

 
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&nbsp;&nbsp;This year the collaboration of our team with SCU_China team and UESTC-software team was very smoothly as expected. We helped each other from the aspects of experimental design, experiment results verification and software to reach the iGEM spirit as resource sharing. The following is a detailed description.
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&nbsp;&nbsp;This year the collaboration of our team with SCU_China team and UESTC-software team were very smoothly as expected. We helped each other from the aspects of experimental design, experiment results verification and software to reach the iGEM spirit of resource sharing. The following is a detailed description.
 
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2015年，我们与SCU_China, UESTC-software等队伍深入交流，实现了资源共享，团结协作，形成了很好的合作互助氛围。并且成功的帮助SCU_China完成其项目中关于核糖体结合位点（RBS）的预测及实验论证的工作。
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In 2015 iGEM, we CHINA_CD_UESTC team collaborated with SCU_China team and UESTC-software team. We communicated several times, helped each other and shared our resources, which generated a great atmosphere of cooperation and mutual assistance. Indeed, we helped SCU_China team finish the prediction and experiment design about RBS in their project.
 
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<img src="https://static.igem.org/mediawiki/2015/c/c6/CHINA_CD_UESTC_COLLABORATION05.jpg" width="70%" height="80%"></div>
 
<img src="https://static.igem.org/mediawiki/2015/c/c6/CHINA_CD_UESTC_COLLABORATION05.jpg" width="70%" height="80%"></div>
 
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<p>在这过程中我们队伍进行了两方面的工作:</p>
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<p>In this process, our team had completed two aspects of the collaboration work:</p>
 
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<h5>（1）帮助SCD_China设计实验，完成RBS预测结果的实验论证。</h5>
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<h5>(1) Help SCU_China team to design the experiments and complete the experimental demonstration of RBS forecast outcomes.</h5>
 
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<h5>（2）给UESTC-software 开发SD Finder软件提供了核糖体结合位点（RBS）预测的理论支持。</h5>
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<h5>(2) Provide the theoretical support of developing SD Finder software aiming to predict the ribosome binding site (RBS) position to UESTC-software team.</h5>
 
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<h2>帮助SCU_China设计实验</h2>
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<h2>Design experiments for SCU_China team</h2>
 
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我们为四川大学iGEM队伍设计了实验，完成RBS预测结果的实验论证。为实现找到未知的RBS位点、并将其标准化的目标。我们为四川大学设计了以下实验：
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We helped SCU_China team design the experiment. In order to find the unknown RBS position and standard it, we designed the folowing experiments for them:
 
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<p id="pic_title">1、nifD基因前RBS的验证</p>
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<p id="pic_title">1. Demonstration of RBS located in upstream of the gene <i>nifD</i></p>
 
<img src="https://static.igem.org/mediawiki/2015/e/ea/CHINA_CD_UESTC_COLLABORATION01.png" width="60%">
 
<img src="https://static.igem.org/mediawiki/2015/e/ea/CHINA_CD_UESTC_COLLABORATION01.png" width="60%">
 
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<p id="pic_title">2、nifD基因前RBS的突变验证</p>
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<p id="pic_title">2. Mutant demonstration of RBS located in upstream of the gene <i>nifD</i></p>
 
<img src="https://static.igem.org/mediawiki/2015/8/8b/CHINA_CD_UESTC_COLLABORATION02.png" width="60%">
 
<img src="https://static.igem.org/mediawiki/2015/8/8b/CHINA_CD_UESTC_COLLABORATION02.png" width="60%">
 
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<p id="pic_title">3、nifK基因前RBS的验证</p>
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<p id="pic_title">3. Demonstration of RBS located in upstream of the gene <i>nifK</i></p>
 
<img src="https://static.igem.org/mediawiki/2015/7/79/CHINA_CD_UESTC_COLLABORATION03.png" width="60%">
 
<img src="https://static.igem.org/mediawiki/2015/7/79/CHINA_CD_UESTC_COLLABORATION03.png" width="60%">
 
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<p id="pic_title">4、nifK基因前RBS的突变验证</p>
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<p id="pic_title">4. Mutant demonstration of RBS located in upstream of the gene <i>nifK</i></p>
 
<img src="https://static.igem.org/mediawiki/2015/8/84/CHINA_CD_UESTC_COLLABORATION04.png" width="60%">
 
<img src="https://static.igem.org/mediawiki/2015/8/84/CHINA_CD_UESTC_COLLABORATION04.png" width="60%">
 
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<p>CHINA_CD_UESTC最终设计并完成了这些实验，很好的帮助SCU_China实现了RBS预测及标准化的工作。</p>
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<p>CHINA_CD_UESTC team finally completed the design of these experiments, which helped SCU_China team accomplish the work of forecast RBS position.</p>
  
 
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<h2>SD Finder理论支持</h2>
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<h2>SD Finder Theoretical Support</h2>
 
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UESTC-software为四川大学开发的核糖体结合位点（RBS）的预测软件，SD Finder，是在我们的理论知识支持下开发的。我们两支队伍的成员很好的弥补了双方在理论和软件技术的不足，协作完成了SD Finder，用于in helping user predict the ORF and SD sequence of DNA sequence.软件链接如下：
+
It was in our theoretical knowledge support that UESTC-software team helped SCU_China team develop a RBS prediction software which named SD Finder. The two teams, CHINA_CD_UESTC team and UESTC-software team, made up deficiencies in theoretical support and software technology to each other and cooperated to complete the SD Finder smoothly. The main function of SD Finder is helping user predict the ORF and SD sequence of DNA sequence. Software links below:
 
</p>
 
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<a href="http://cefg.cn/Igem2015/mccap-server/app/tools/sdFinder.php">
 
<a href="http://cefg.cn/Igem2015/mccap-server/app/tools/sdFinder.php">
 
<p>http://cefg.cn/Igem2015/mccap-server/app/tools/sdFinder.php</p>
 
<p>http://cefg.cn/Igem2015/mccap-server/app/tools/sdFinder.php</p>
 
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<img src="https://static.igem.org/mediawiki/2015/6/68/CHINA_CD_UESTC_COLLABORATION06.png" width="80%" height="80%"></div>
 
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Latest revision as of 00:33, 18 September 2015


<!DOCTYPE html>

COLLABORATIONS

  This year the collaboration of our team with SCU_China team and UESTC-software team were very smoothly as expected. We helped each other from the aspects of experimental design, experiment results verification and software to reach the iGEM spirit of resource sharing. The following is a detailed description.

In 2015 iGEM, we CHINA_CD_UESTC team collaborated with SCU_China team and UESTC-software team. We communicated several times, helped each other and shared our resources, which generated a great atmosphere of cooperation and mutual assistance. Indeed, we helped SCU_China team finish the prediction and experiment design about RBS in their project.

In this process, our team had completed two aspects of the collaboration work:

  • (1) Help SCU_China team to design the experiments and complete the experimental demonstration of RBS forecast outcomes.
  • (2) Provide the theoretical support of developing SD Finder software aiming to predict the ribosome binding site (RBS) position to UESTC-software team.

Design experiments for SCU_China team

We helped SCU_China team design the experiment. In order to find the unknown RBS position and standard it, we designed the folowing experiments for them:

1. Demonstration of RBS located in upstream of the gene nifD

2. Mutant demonstration of RBS located in upstream of the gene nifD

3. Demonstration of RBS located in upstream of the gene nifK

4. Mutant demonstration of RBS located in upstream of the gene nifK

CHINA_CD_UESTC team finally completed the design of these experiments, which helped SCU_China team accomplish the work of forecast RBS position.

SD Finder Theoretical Support

It was in our theoretical knowledge support that UESTC-software team helped SCU_China team develop a RBS prediction software which named SD Finder. The two teams, CHINA_CD_UESTC team and UESTC-software team, made up deficiencies in theoretical support and software technology to each other and cooperated to complete the SD Finder smoothly. The main function of SD Finder is helping user predict the ORF and SD sequence of DNA sequence. Software links below:

http://cefg.cn/Igem2015/mccap-server/app/tools/sdFinder.php