Difference between revisions of "Team:Yale/standards"
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| − | <li>Validate Bricks= Successfully finished! Please look at part #??? in <a href="https://2015.igem.org/Team:Yale/parts"> our parts section! </a></li> | + | <li>Validate Bricks= Successfully finished! Please look at part #??? in <a href="https://2015.igem.org/Team:Yale/parts"> our parts section! </a> In the parts section should be detailed information on how each of the seven promoter-citrine construct BioBricks effectively produce citrine when ported into an organism that has the machinery required for each promoter.</li> |
| − | <li>Submit Parts= Successfully finished! Please look at part #???? in <a href="https://2015.igem.org/Team:Yale/parts"> our parts section! </a></li> | + | <li>Submit Parts= Successfully finished! Please look at part #???? in <a href="https://2015.igem.org/Team:Yale/parts"> our parts section! </a> Along with the lac-citrine construct detailed in the bronze section below, our team also constructed six more promoter-citrine constructs. Once spliced into your chosen shuttle vector and transformed into your non model organism, these constructs allow for easy testing. If citrine is produced in your non model organism, your organism contains the machinery necessary to promote transcription with the chosen promoter! If no citrine is produced, then the chosen operator does not cause transcription in your organism. Please look at the linked section for more details. Also, we were able to construct three BioBricks, each containing a different recombinase gene. While these BioBricks were not experimentally validated, they should lead to the production of recombinase proteins that may help increase the efficiency of MAGE in various non-model organisms! </li> |
| − | <li>Human Practices= Successfully finished! Please look at our <a href="https://2015.igem.org/Team:Yale/practices"> our practices section! </a></li> | + | <li>Human Practices= Successfully finished! Please look at our <a href="https://2015.igem.org/Team:Yale/practices"> our practices section! </a> In order to spread greater knowledge of synthetic biology, our iGEM team hosted a talk at the Summer Science Research Institute (SSRI), a summer program for high school students interested in the sciences. At this talk, three of our team members covered the basic history of synthetic biology, common techniques of synthetic biology, common uses of this field of research in industry, and, finally, covered our own research as a specific case study of synthetic biology research. Along with this, we set up a public presentation at Yale's Center for Engineering Innovation and Design in order to address the ethical considerations of synthetic biology and counter misinformation that leads to public paranoia over synthetic biology. In order to do this, we constructed a presentation covering GMOs, their uses, their benefits, and their drawbacks. We also presented information on our own project to show the size of the field and show that synthetic biology does not only deal with modifying crops. After presenting our information, our team members moderated a discussion covering the subject.</li> |
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<li>Make clear attributions for work done by others= Successfully Finished! | <li>Make clear attributions for work done by others= Successfully Finished! | ||
| − | Please look at <a href="https://2015.igem.org/Team:Yale/ | + | Please look at <a href="https://2015.igem.org/Team:Yale/Attributions"> our Attributions section</a> to see the full last of people and companies that helped our team!</li> |
<li>Make at least one new BioBrick Part= Successfully Finished! | <li>Make at least one new BioBrick Part= Successfully Finished! | ||
| − | Please look at <a href="https://2015.igem.org/Team:Yale/parts"> our parts section! </a> | + | Please look at <a href="https://2015.igem.org/Team:Yale/parts"> our parts section! </a> For our initial BioBrick part, we created and submitted a Biobrick containing the lac promoter and the citrine gene. This Biobrick can be used to test the expression of the lac promoter in various non model organisms. This is just one of seven constructs built by our team to help other iGEM teams test the functionality of common promoters in their chosen non model organism. |
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Revision as of 02:33, 18 September 2015
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Standards
Gold
- Improve Function of Existing BioBrick
- Help Any Other Team
- Do Outreach
Silver
- Validate Bricks= Successfully finished! Please look at part #??? in our parts section! In the parts section should be detailed information on how each of the seven promoter-citrine construct BioBricks effectively produce citrine when ported into an organism that has the machinery required for each promoter.
- Submit Parts= Successfully finished! Please look at part #???? in our parts section! Along with the lac-citrine construct detailed in the bronze section below, our team also constructed six more promoter-citrine constructs. Once spliced into your chosen shuttle vector and transformed into your non model organism, these constructs allow for easy testing. If citrine is produced in your non model organism, your organism contains the machinery necessary to promote transcription with the chosen promoter! If no citrine is produced, then the chosen operator does not cause transcription in your organism. Please look at the linked section for more details. Also, we were able to construct three BioBricks, each containing a different recombinase gene. While these BioBricks were not experimentally validated, they should lead to the production of recombinase proteins that may help increase the efficiency of MAGE in various non-model organisms!
- Human Practices= Successfully finished! Please look at our our practices section! In order to spread greater knowledge of synthetic biology, our iGEM team hosted a talk at the Summer Science Research Institute (SSRI), a summer program for high school students interested in the sciences. At this talk, three of our team members covered the basic history of synthetic biology, common techniques of synthetic biology, common uses of this field of research in industry, and, finally, covered our own research as a specific case study of synthetic biology research. Along with this, we set up a public presentation at Yale's Center for Engineering Innovation and Design in order to address the ethical considerations of synthetic biology and counter misinformation that leads to public paranoia over synthetic biology. In order to do this, we constructed a presentation covering GMOs, their uses, their benefits, and their drawbacks. We also presented information on our own project to show the size of the field and show that synthetic biology does not only deal with modifying crops. After presenting our information, our team members moderated a discussion covering the subject.
Bronze
- Make clear attributions for work done by others= Successfully Finished! Please look at our Attributions section to see the full last of people and companies that helped our team!
- Make at least one new BioBrick Part= Successfully Finished! Please look at our parts section! For our initial BioBrick part, we created and submitted a Biobrick containing the lac promoter and the citrine gene. This Biobrick can be used to test the expression of the lac promoter in various non model organisms. This is just one of seven constructs built by our team to help other iGEM teams test the functionality of common promoters in their chosen non model organism.
Yale iGEM 2015
Main Campus:
Yale Department of Molecular, Cellular & Developmental Biology
Attn: Farren Isaacs/iGEM
219 Prospect St
PO Box 208103
New Haven, CT 06520
Tel. +1(203) 432-3783
E-mail: igem.yale@gmail.com
© Yale iGEM 2015