THE TEAM

Research

Main Project: Jack Kwon, Kevin Tao

Foldscope:Lily Ge, Chris Jackson

Labwork

Hanna Minot, Sarah Rupert, Lauren Pan, Lauren Hong, Jack Kwon, Kevin Tao, Julia Leveille, Alyssa Franklin

Wiki

Neha Balachandran, Lily Ge

Design

Spiritwear: Elynna Chang

Banner: Lily Ge

Poster: Alyssa Franklin, Lauren Hong, Julia Leveille

Flyer: Hanna Minot

Handouts: Hanna Minot, Chris Jackson, Lauren Hong

Outreach

Atlanta Science Festival: Elynna Chang

Next Generation Focus: Noor Sohal

Lab Hacks, Oyster Farms and Wheat Stripe Rust: Lauren Pan

Keep Charlie Moving: Whole Team

Operation Christmas: Whole Team

Presentation

Presenters:Noor Sohal, Elynna Chang, Jack Kwon

Script and other help: Kevin Tao, Lily Ge

Acknowledgements

General Support

Mark Styczynski- Associate Professor at Georgia Institute of Technology

Dan Watstein- graduate student " "

GATech iGEM team

Our graduated seniors from 2015 who have gone on to greater fields; Christina Bae, Marybeth Koepsell, Kaitlyn Martin, Nimisha Vasandani, Chris Waites, Rebecca Yan and Justin Yoo. We couldn't be here without you!

Chitin Support

Snapgene- software for sequence analysis

IDT- synthesis of g blocks

Mrs. Standeven's Biotech Classes

Foldscope Support

Prakash Lab Stanford University- provided foldscopes in their Beta testing

Rosco Laboratories- provided free filters

Funding Support

Lambert High School

Johns Creek Chick fil A

National Science Foundation Grant #1254382

Wiki Support

Sai Kilaru- wiki formatting

Dugan Walker- graphics of constructs

Outreach Support

Next Generation Focus/ Grace Chapel Hill Church

Atlanta Science Festival

Keep Charlie Moving/ Charlie & Family

Operation Christmas

Collaboration

[collaboration with GT iGEM team]

Sources

Anitha, A., S. Sowmya, P.T.S. Kumar, S. Deepthi, K.P. Chennazhi, H. Ehrlich, M. Tsurkan, and R. Jayakumar. Chitin and chitosan in selected biomedical applications. Progress in Polymer Science.

Christodoulidou, A., V. Bouriotis, and G. Thireos. 1996. Two sporulation-specific chitin deacetylase-encoding genes are required for the ascospore wall rigidity of Saccharomyces cerevisiae. The Journal of biological chemistry. 271:31420-31425.

Christodoulidou, A., P. Briza, A. Ellinger, and V. Bouriotis. 1999a. Yeast ascospore wall assembly requires two chitin deacetylase isozymes. FEBS letters. 460:275-279.

Jarmila, V., and E. Vavrikova. 2011. Chitosan derivatives with antimicrobial, antitumour and antioxidant activities--a review. Current pharmaceutical design. 17:3596-3607.

Leceta, I., P. Guerrero, S. Cabezudo, and K.d.l. Caba. 2013. Environmental assessment of chitosan-based films. Journal of Cleaner Production. 41:312-318.

Liu, N., X.-G. Chen, H.-J. Park, C.-G. Liu, C.-S. Liu, X.-H. Meng, and L.-J. Yu. 2006. Effect of MW and concentration of chitosan on antibacterial activity of Escherichia coli. Carbohydrate Polymers. 64:60-65.

Martínez, J.P., M.P. Falomir, and D. Gozalbo. 2001. Chitin: A Structural Biopolysaccharide. In eLS. John Wiley & Sons, Ltd.

Martinou, A., D. Koutsioulis, and V. Bouriotis. 2003. Cloning and expression of a chitin deacetylase gene (CDA2) from Saccharomyces cerevisiae in Escherichia coli: Purification and characterization of the cobalt-dependent recombinant enzyme. Enzyme and Microbial Technology. 32:757-763.

Mendelovits, A., T. Prat, Y. Gonen, and G. Rytwo. 2012. Improved colorimetric determination of chitosan concentrations by dye binding. Applied spectroscopy. 66:979- 982.

Moussa, S.H., A.A. Tayel, and A.I. Al-Turki. 2013. Evaluation of fungal chitosan as a biocontrol and antibacterial agent using fluorescence-labeling. International Journal of Biological Macromolecules. 54:204-208.

Tsigos, I., A. Martinou, D. Kafetzopoulos, and V. Bouriotis. 2000. Chitin deacetylases: new, versatile tools in biotechnology. Trends in Biotechnology. 18:305-312.

Zakrzewska, A., A. Boorsma, S. Brul, K.J. Hellingwerf, and F.M. Klis. 2005. Transcriptional response of Saccharomyces cerevisiae to the plasma membrane- perturbing compound chitosan. Eukaryotic cell. 4:703-715.

Zhang, H., R. Li, and W. Liu. 2011. Effects of Chitin and Its Derivative Chitosan on Postharvest Decay of Fruits: A Review. International Journal of Molecular Sciences. 12: 917-934.