Difference between revisions of "Team:CHINA CD UESTC/Method"
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<h3>Q2: Where are the backbone vectors from?</h3> | <h3>Q2: Where are the backbone vectors from?</h3> | ||
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− | All backbone vectors are purchased from Biotech Corp. They are | + | All backbone vectors are purchased from Biotech Corp. They are pET-28a(Figure 2), pCDFDuet-1(Figure 3) and pACYCDuet-1(Figure 4), the first two aims to carry gene clusters that realize magnetosome generating and the last one is for putting the genes (<i>mamW</i> + <i>RFP</i> + <i>laccase</i>) together. |
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<img src="https://static.igem.org/mediawiki/2015/b/b3/CHINA_CD_UESTC_METHOD02.png" width="60%"> | <img src="https://static.igem.org/mediawiki/2015/b/b3/CHINA_CD_UESTC_METHOD02.png" width="60%"> | ||
<p id="pic_illustration"> | <p id="pic_illustration"> | ||
− | <strong>Figure 2.</strong> This vector backbone ( | + | <strong>Figure 2.</strong> This vector backbone (pET-28a) will be inserted <i>mamAB</i></p> |
</div> | </div> | ||
<div class="project_pic"> | <div class="project_pic"> |
Latest revision as of 02:52, 18 September 2015
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METHOD
We present fundamental details on various methods such as vector design, domain linker selection and choose of restriction enzyme sites used in the experiment on this page. Any questions or advice are welcomed at any time.