Characterized biofilm properties of B. subtilis. Natto, 3610 ComI, 168, JH642 and PY79
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| + | Bacillus Subtilis & Growth Method | ||
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| + | <i>Drip flow experiment/ directed evolution</i> | ||
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| + | To increase the robustness of the biofilm in a natural way the drip and continuous flow experiment had been performed. A biofilm was put in an angle of 15 degrees and a constant flow of medium was flowing over it. Only the cells with a high enough robustness were sticking to the carrier material. Unfortunately only one biofilm at a time was grown in this set up and it was requiring lots of growth medium. Several hundred plates were needed for our experiments so the drip and continuous flow experiments were stopped after a while to save time and money. The choice afterward was between submerged and at the air surface | ||
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Revision as of 02:56, 18 September 2015
Overview
Demonstrated ion-selectivity of the B. subtilis biofilm and improved it with our biobricks.
Modeled y-PGA as a Cation Exchange membrane using Molecular Dynamics
Designed drip and continuous flow method to grow more robust biofilms.
Created easy cheap test to measure rigidity of biofilms.
Created new backbone(K1597001) for integration into ThrC locus in B. subtilis and validated it.
Constructs created for TasA, BslA, Abrb KO, SlrR, y-PGA and ProH
Created biobricks to increase the robustness.
Validated ThrC(K1597001), TasA(K1597002) and ProH (K1597000)
Created future scenario’s about GMO’s and sustainability and a cardgame for education
Bacillus Subtilis & Growth Method
Drip flow experiment/ directed evolution
To increase the robustness of the biofilm in a natural way the drip and continuous flow experiment had been performed. A biofilm was put in an angle of 15 degrees and a constant flow of medium was flowing over it. Only the cells with a high enough robustness were sticking to the carrier material. Unfortunately only one biofilm at a time was grown in this set up and it was requiring lots of growth medium. Several hundred plates were needed for our experiments so the drip and continuous flow experiments were stopped after a while to save time and money. The choice afterward was between submerged and at the air surface