Difference between revisions of "Team:NYU-AD/Notebook/Week4"

(Created page with "{{NYU-AD-Header2}} <html> <style> h1{ font-size: 30px; } .text { color:black; font-family: "source_sans_proregular", sans-serif; font-size: 1.3em; line-h...")
 
 
(One intermediate revision by the same user not shown)
Line 2: Line 2:
 
<html>
 
<html>
 
<style>
 
<style>
−
h1{
+
.text {
−
font-size: 30px;
+
−
}
+
−
.text {
+
 
color:black;
 
color:black;
 
font-family: "source_sans_proregular", sans-serif;
 
font-family: "source_sans_proregular", sans-serif;
Line 12: Line 9:
 
margin: 0 0 1.4em;
 
margin: 0 0 1.4em;
 
}
 
}
−
.subtitle {
+
h1 {
−
margin-top: 40px;
+
font-family: "source_sans_proregular", sans-serif;
−
margin-bottom: 20px;
+
color: black;
−
font-family: "source_sans_proregular", sans-serif;
+
text-align:center;
−
color: black;
+
border-bottom:none;
−
font-size: 25px;
+
color: #3c948b;
−
text-decoration: none;
+
font-style:bold;
−
border:0;
+
font-size: 30px;
−
}
+
}
 
table {
 
table {
 
border-spacing: 5px;
 
border-spacing: 5px;
Line 48: Line 45:
 
.figure{
 
.figure{
 
position:relative;
 
position:relative;
−
display:inline-block;
+
width:75%;
−
margin-right:30px;
+
margin:auto;
−
width:1000px;
+
 
padding:15px;
 
padding:15px;
 
background:#F8F8F8;
 
background:#F8F8F8;
Line 60: Line 56:
 
</style>
 
</style>
 
<div class="figure">
 
<div class="figure">
−
<p class="subtitle">Week 4</p>
+
<h1>Week 4</h1>
 
<h2>26/08/15 </h2>
 
<h2>26/08/15 </h2>
 
<ul>
 
<ul>

Latest revision as of 03:11, 18 September 2015

Week 4

26/08/15

  • Performed ligation of the promoter and tnaA
  • Amplifying the tnaA and tnaB sequence. .
  • Added 200ul of water (DNA- RNA free) into each tube
  • Performed the Nano Drop . The concentration of both tubes is approximately 7-8ug/ul.

27/08/2015

  • Nanodrop to verify concentration of resuspended DNA
  • Nuclease- free H2O used to resuspend the DNA was found to be contaminated with 4.8ug/ul of DNA
  • The actual DNA content of plasmids are around 6-8 ug/ul on average
  • We decided to proceed with the transformation since protocol specified 10ug- 100ug of DNA.

28/08/2015

  • We received the tnaa and the tnab tubes from IDT on the 26/08/15
  • Resuspended the tnaa and the tnab sequence by adding 200ul of water ( DNAse and RNAase free) into each tube.

29/08/2015

  • Nanodropped the DNA to verify the concentration of resuspended DNA. The Nuclease-free water that was used to reuspend the DNA was found to be contaminated with 4.8ng/ul of DNA. The actual DNA content of the plasmids is around 6-8ng/ul on average.
  • We still proceeded with the transformation of the DNA since the protocol specified 10ug-1000ug of DNA.